2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

2-Phosphoglycerate (2-PG) also known as phosphoglycerate is an intermediate metabolite in the glycolytic pathway. It results from the conversion of 3-phosphoglycerate to 2-phosphoglycerate by the enzyme phosphoglycerate mutase. It then converts into phosphoenolpyruvate (PEP) under the action of enolase an enzyme important for this transformation. 2-Phosphoglycerate is small in mass and expressively found in cellular cytoplasm where glycolysis takes place.

Biological function summary

2-phosphoglycerate plays a role in energy metabolism as an integral part of the glycolytic pathway. It exists within the cellular environment facilitating cellular respiration by progressing the glycolytic pathway towards the generation of pyruvate. It does not form part of a large protein complex functioning independently to ensure progression through glycolysis.

Pathways

2-phosphoglycerate is mainly involved in the glycolysis pathway that helps in the breakdown of glucose to pyruvate for energy production. The transformation from 2-PG to PEP via enolase signifies its role in energy yield within cells. It is closely associated with proteins like enolase and phosphoglycerate mutase which directly interact with it in the metabolic pathway sequence.

Associated diseases and disorders

Disturbances in the equilibrium of 2-phosphoglycerate levels can contribute to metabolic disorders such as glycolytic enzyme deficiencies. While rare these enzyme deficiencies can impact energy metabolism resulting in conditions like hemolytic anemia. Alterations in the glycolytic pathway involving this metabolite could affect the function of enzymes like enolase which may have further consequences in energy-related disorders.