JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174097

2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric)

Be the first to review this product! Submit a review

|

(6 Publications)

2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit.
3 Images
Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)
  • FuncS

Supplier Data

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)

2PG Standard Curve (Fluoroimetric).

Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by fluorometric reading. Please not this data is example data only.

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)
  • FuncS

Supplier Data

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)

2PG Standard Curve (Colorimetric).

Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)
  • FuncS

Supplier Data

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)

Measurement of 2PG levels.

Measurement of 2PG level in rat liver, heart and muscle lysate (200 μg protein each). Assays were performed according to kit protocol. This is example data only.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cell culture extracts, Suspension cells, Tissue Extracts, Adherent cells

Assay type

Quantitative

Assay Platform

Microplate reader

style
left-column

Product details

Abcam's 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K778 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit. K778-100 is the same size as the 100 test size of ab174097.

2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab174097-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PEP Converter Mix", "number":"AB174097-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2-Phosphoglycerate", "number":"AB174097-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2PG Enzyme Mix", "number":"AB174097-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB174097-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB174097-CMP03", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB174097-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

2-Phosphoglycerate (2-PG) also known as phosphoglycerate is an intermediate metabolite in the glycolytic pathway. It results from the conversion of 3-phosphoglycerate to 2-phosphoglycerate by the enzyme phosphoglycerate mutase. It then converts into phosphoenolpyruvate (PEP) under the action of enolase an enzyme important for this transformation. 2-Phosphoglycerate is small in mass and expressively found in cellular cytoplasm where glycolysis takes place.
Biological function summary

2-phosphoglycerate plays a role in energy metabolism as an integral part of the glycolytic pathway. It exists within the cellular environment facilitating cellular respiration by progressing the glycolytic pathway towards the generation of pyruvate. It does not form part of a large protein complex functioning independently to ensure progression through glycolysis.

Pathways

2-phosphoglycerate is mainly involved in the glycolysis pathway that helps in the breakdown of glucose to pyruvate for energy production. The transformation from 2-PG to PEP via enolase signifies its role in energy yield within cells. It is closely associated with proteins like enolase and phosphoglycerate mutase which directly interact with it in the metabolic pathway sequence.

Disturbances in the equilibrium of 2-phosphoglycerate levels can contribute to metabolic disorders such as glycolytic enzyme deficiencies. While rare these enzyme deficiencies can impact energy metabolism resulting in conditions like hemolytic anemia. Alterations in the glycolytic pathway involving this metabolite could affect the function of enzymes like enolase which may have further consequences in energy-related disorders.
style
left-column

Product protocols

style
left-column

Target data

See full target information 2-Phosphoglyceric acid
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Plant, cell & environment 48:3159-3170 PubMed39718138

2024

Carboxylation and Oxygenation Kinetics and Large Subunit (rbcL) DNA Sequences for Rubisco From Two Ecotypes of Plantago lanceolata L. That Are Native to Sites Differing in Atmospheric CO Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Shi,Nathan M Hannon,Arnold J Bloom

Biology direct 19:83 PubMed39294751

2024

ZFP64 drives glycolysis-mediated stem cell-like properties and tumorigenesis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Sun,Jinquan Liu,Yudong Hou,Jianheng Bao,Teng Wang,Longbi Liu,Yidan Zhang,Rui Zhong,Zhenxuan Sun,Yan Ye,Jintao Liu

Nature communications 15:6755 PubMed39117659

2024

Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Hongchao Cao,Jing Wang,Areeg Elmusrati,Bing Han,Wei Chen,Ping Zhou,Xiyao Li,Stephen Keysar,Antonio Jimeno,Cun-Yu Wang

The Journal of biological chemistry 298:102561 PubMed36198360

2022

Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Jiang,Ting Wang,Dandan Sheng,Chaoqiang Han,Tian Xu,Peng Zhang,Weiyi You,Weiwei Fan,Zhiyong Zhang,Tengchuan Jin,Xiaotao Duan,Xiao Yuan,Xing Liu,Kaiguang Zhang,Ke Ruan,Jue Shi,Jing Guo,Aoxing Cheng,Zhenye Yang

Nature communications 9:3427 PubMed30143643

2018

Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsoo Song,In-Jeoung Baek,Churl-Hong Chun,Eun-Jung Jin

Oncotarget 8:113516-113530 PubMed29371926

2017

A natural inhibitor of kidney-type glutaminase: a withanolide from with potent anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Canrong Wu,Mengzhu Zheng,Suyu Gao,Shanshan Luan,Li Cheng,Liqing Wang,Jiachen Li,Lixia Chen,Hua Li
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB65342

Pyruvate Assay Kit

Functional Studies - Pyruvate Assay Kit (AB65342)

  • 5
  • 2
Assay Kits

AB155898

Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric)

Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)

  • 0
  • 0
Assay Kits

AB65348

NAD/NADH Assay Kit (Colorimetric)

Biochemical assay - NAD/NADH Assay Kit (Colorimetric) (AB65348)

  • 0
  • 0
Assay Kits

AB83432

Pyruvate Kinase Assay Kit

Functional Studies - Pyruvate Kinase Assay Kit (AB83432)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com