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AB273269

3' to 5' Exonuclease Activity Assay Kit (Fluorometric)

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(2 Publications)

3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3' exonuclease activities in a wide variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Three-prime repair exonuclease 1, 3'-5' exonuclease TREX1, Deoxyribonuclease III, DNase III, TREX1

4 Images
Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)
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Supplier Data

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)

3' to 5' Exonuclease Kinetics.

Kinetic curves of rat liver extract (40.6 μg), HeLa cell lysate(12.2 μg) and E.coli (0.642 μg).

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)
  • FuncS

Supplier Data

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)

3' to 5' Exonuclease Activity.

Specific activity of the probe to 3' to 5' Exonuclease (Exonucleae III) in contrast to DNAase I. Same units of proteins were added as defined by the commercially available values.

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)
  • FuncS

Supplier Data

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)

3' to 5' Exonuclease Activity in different Sample types.

Specific activity from rat liver extract (0.0206 μU/μg). HeLa cell lysate (0.0841 μU/μg) and E.coli crude extract (0.9071 μU/μg). Assays performed according to the kit protocol.

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)
  • FuncS

Supplier Data

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Fluorescence Standard Curve.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cell culture extracts, Suspension cells, Tissue Extracts, Adherent cells

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3' exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 μU of exonuclease activity.

This product is manufactured by BioVision, an Abcam company and was previously called K175 3' to 5' Exonuclease Activity Assay (Fluorometric). K175-100 is the same size as the 100 test size of ab273269.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273269-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Exonuclease Probe", "number":"AB273269-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Half Area Plate (with lid)", "number":"AB273269-CMP01", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Fluorescence Standard II", "number":"AB273269-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Exonuclease Positive Control", "number":"AB273269-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Exonuclease Assay Buffer", "number":"AB273269-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 3' to 5' exonuclease also known as exonuclease III is an important enzyme that removes nucleotides from the ends of a DNA strand in the 3' to 5' direction. Its role in DNA repair and proofreading contributes to maintaining genomic stability. Exonuclease III is expressed primarily in the cell nucleus and has a molecular mass of about 31 kDa. The enzyme's activity is essential for DNA replication and repair processes and it displays strong exonuclease activity.
Biological function summary

Exonuclease removes mismatched bases during DNA proofreading therefore ensuring the accuracy of DNA replication. It acts as part of the DNA polymerase complex where it helps maintain the fidelity of DNA synthesis. The enzyme also participates in base excision repair (BER) pathways a critical mechanism for correcting DNA single-strand breaks. By participating in these processes exonuclease plays a part in preserving the genome integrity of the cell.

Pathways

Exonuclease 3' to 5' activity is important in the mismatch repair (MMR) and nucleotide excision repair (NER) pathways. In these pathways the exonuclease partners with DNA polymerase and other nucleases like endonucleases ensuring the replacement of incorrect or damaged nucleotides. It works synergistically with proteins such as DNA ligase which seals the nicks in the DNA phosphodiester backbone. Through these collaborative interactions exonuclease supports the dynamic regulation of DNA integrity.

Exonuclease's function intertwines with oncogenesis and neurodegenerative diseases. Its deficiency or malfunction can lead to conditions like cancer due to the accumulation of mutations from failed DNA repair mechanisms. The 3' to 5' exonuclease activity insufficiency is linked with proteins such as proliferating cell nuclear antigen (PCNA) which are vital in DNA replication and repair processes. In neurodegenerative diseases compromised exonuclease activity leads to an increase in DNA damage contributing to disease progression.
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Product protocols

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Target data

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini (PubMed : 10391904, PubMed : 10393201, PubMed : 17293595). Prevents cell-intrinsic initiation of autoimmunity (PubMed : 10391904, PubMed : 10393201, PubMed : 17293595). Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements (PubMed : 10391904, PubMed : 10393201, PubMed : 17293595). Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability : its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation (PubMed : 33476576). Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation (PubMed : 33476576). Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon (PubMed : 33476576). Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates (PubMed : 18045533). Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light (PubMed : 23993650). During GZMA-mediated cell death, contributes to DNA damage in concert with NME1 (PubMed : 16818237). NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair (PubMed : 16818237).
See full target information TREX1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0305962 PubMed39178223

2024

A novel TREX1 inhibitor, VB-85680, upregulates cellular interferon responses.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Flowers,Brenda A Petronella,Michael S McQueney,Barbara Fanelli,Warren Eisenberg,Albert Uveges,Allison L Roden,Scott Salowe,Venu Bommireddy,Jeffrey J Letourneau,Chia-Yu Huang,James R Beasley

Nature communications 15:6685 PubMed39107301

2024

Heterozygous de novo dominant negative mutation of REXO2 results in interferonopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Idiiatullina,Mahmoud Al-Azab,Meng Lin,Katja Hrovat-Schaale,Ziyang Liu,Xiaotian Li,Caiqin Guo,Xixi Chen,Yaoying Li,Song Gao,Jun Cui,Wenhao Zhou,Li Liu,Yuxia Zhang,Seth L Masters
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