3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3' exonuclease activities in a wide variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Cell culture extracts, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
Enzyme activity (quantitative)
Target data
Function
Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Alternative names
Three-prime repair exonuclease 1, 3'-5' exonuclease TREX1, Deoxyribonuclease III, DNase III, TREX1
What's included?
Recommended products
3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3' exonuclease activities in a wide variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Three-prime repair exonuclease 1, 3'-5' exonuclease TREX1, Deoxyribonuclease III, DNase III, TREX1
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Cell culture extracts, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3' exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 μU of exonuclease activity.
This product is manufactured by BioVision, an Abcam company and was previously called K175 3' to 5' Exonuclease Activity Assay (Fluorometric). K175-100 is the same size as the 100 test size of ab273269.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 3' to 5' exonuclease also known as exonuclease III is an important enzyme that removes nucleotides from the ends of a DNA strand in the 3' to 5' direction. Its role in DNA repair and proofreading contributes to maintaining genomic stability. Exonuclease III is expressed primarily in the cell nucleus and has a molecular mass of about 31 kDa. The enzyme's activity is essential for DNA replication and repair processes and it displays strong exonuclease activity.
- Biological function summary
Exonuclease removes mismatched bases during DNA proofreading therefore ensuring the accuracy of DNA replication. It acts as part of the DNA polymerase complex where it helps maintain the fidelity of DNA synthesis. The enzyme also participates in base excision repair (BER) pathways a critical mechanism for correcting DNA single-strand breaks. By participating in these processes exonuclease plays a part in preserving the genome integrity of the cell.
- Pathways
Exonuclease 3' to 5' activity is important in the mismatch repair (MMR) and nucleotide excision repair (NER) pathways. In these pathways the exonuclease partners with DNA polymerase and other nucleases like endonucleases ensuring the replacement of incorrect or damaged nucleotides. It works synergistically with proteins such as DNA ligase which seals the nicks in the DNA phosphodiester backbone. Through these collaborative interactions exonuclease supports the dynamic regulation of DNA integrity.
- Associated diseases and disorders
Exonuclease's function intertwines with oncogenesis and neurodegenerative diseases. Its deficiency or malfunction can lead to conditions like cancer due to the accumulation of mutations from failed DNA repair mechanisms. The 3' to 5' exonuclease activity insufficiency is linked with proteins such as proliferating cell nuclear antigen (PCNA) which are vital in DNA replication and repair processes. In neurodegenerative diseases compromised exonuclease activity leads to an increase in DNA damage contributing to disease progression.
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4 product images
Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269)
3' to 5' Exonuclease Kinetics.
Kinetic curves of rat liver extract (40.6 μg), HeLa cell lysate(12.2 μg) and E.coli (0.642 μg).
Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269)
3' to 5' Exonuclease Activity in different Sample types.
Specific activity from rat liver extract (0.0206 μU/μg). HeLa cell lysate (0.0841 μU/μg) and E.coli crude extract (0.9071 μU/μg). Assays performed according to the kit protocol.
Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269)
3' to 5' Exonuclease Activity.
Specific activity of the probe to 3' to 5' Exonuclease (Exonucleae III) in contrast to DNAase I. Same units of proteins were added as defined by the commercially available values.
Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Fluorescence Standard Curve.
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