3D Tissue Staining Kit – CUBIC (ab316248) enables the easy set-up of 3D tissue imaging using simple protocols and standard laboratory equipment.
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- Nature protocols 14:3506-35372019Advanced CUBIC tissue clearing for whole-organ cell profiling.Applications:IHC-tissue clearingReactive species:Unspecified reactive speciesKatsuhiko Matsumoto,Tomoki T Mitani,Shuhei A Horiguchi,Junichi Kaneshiro,Tatsuya C Murakami,Tomoyuki Mano,Hiroshi Fujishima,Ayumu Konno,Tomonobu M Watanabe,Hirokazu Hirai,Hiroki R UedaPubMed 31748753
- Cell reports 24:2196-2210.e92018Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.Applications:IHC-tissue clearingReactive species:Unspecified reactive speciesKazuki Tainaka,Tatsuya C Murakami,Etsuo A Susaki,Chika Shimizu,Rie Saito,Kei Takahashi,Akiko Hayashi-Takagi,Hiroshi Sekiya,Yasunobu Arima,Satoshi Nojima,Masako Ikemura,Tetsuo Ushiku,Yoshihiro Shimizu,Masaaki Murakami,Kenji F Tanaka,Masamitsu Iino,Haruo Kasai,Toshikuni Sasaoka,Kazuto Kobayashi,Kohei Miyazono,Eiichi Morii,Tadashi Isa,Masashi Fukayama,Akiyoshi Kakita,Hiroki R UedaPubMed 30134179
- Scientific reports 7:92692017CUBIC pathology: three-dimensional imaging for pathological diagnosis.Applications:IHC-tissue clearingReactive species:Unspecified reactive speciesSatoshi Nojima,Etsuo A Susaki,Kyotaro Yoshida,Hiroyoshi Takemoto,Naoto Tsujimura,Shohei Iijima,Ko Takachi,Yujiro Nakahara,Shinichiro Tahara,Kenji Ohshima,Masako Kurashige,Yumiko Hori,Naoki Wada,Jun-Ichiro Ikeda,Atsushi Kumanogoh,Eiichi Morii,Hiroki R UedaPubMed 28839164
- Cell reports 20:236-2502017Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.Applications:IHC-tissue clearingReactive species:Unspecified reactive speciesShimpei I Kubota,Kei Takahashi,Jun Nishida,Yasuyuki Morishita,Shogo Ehata,Kazuki Tainaka,Kohei Miyazono,Hiroki R UedaPubMed 28683317
- Cell 157:726-392014Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.Applications:IHC-tissue clearingReactive species:Unspecified reactive speciesEtsuo A Susaki,Kazuki Tainaka,Dimitri Perrin,Fumiaki Kishino,Takehiro Tawara,Tomonobu M Watanabe,Chihiro Yokoyama,Hirotaka Onoe,Megumi Eguchi,Shun Yamaguchi,Takaya Abe,Hiroshi Kiyonari,Yoshihiro Shimizu,Atsushi Miyawaki,Hideo Yokota,Hiroki R UedaPubMed 24746791
Key facts
- Reactive species
- Mouse
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-tissue clearing | Reactivity Reacts | Dilution info - | Notes - |
What's included?
3D Tissue Staining Kit – CUBIC (ab316248) enables the easy set-up of 3D tissue imaging using simple protocols and standard laboratory equipment.
Key facts
- Reactive species
- Mouse
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
3D Tissue Staining Kit – CUBIC (ab316248) enables the easy set-up of 3D tissue imaging using simple protocols and standard laboratory equipment.
Together with the Tissue Clearing Kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) we provide a versatile tissue clearing and 3D tissue staining method that allows the observation of all cells in organs and the body. The kits are designed for safe and easy use in the lab.
- Practical application of a special tissue staining process that uniformly stains 3D blocks of tissue
- Highly reproducible protocol compatible with various nuclear and antibody stains
The provided protocol solves issues pertaining to safety, waste processing, microscope compatibility, fluorescent protein signal retainability, etc.
To help you get started with tissue clearing, please visit the available data on tested antibodies on this datasheet. Validated antibodies are for key markers and are tested for tissue clearing and staining with whole mouse brains. If you wish to set up tissue clearing with antibodies that we haven’t tested yet, please consult the protocol book for further details.
To learn more about tissue clearing, and tissue clearing methods (including for 3D cell cultures), please review our guide to tissue clearing.
The tissue staining kit uses CUBIC technology.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (ab316248)
After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246), anti-c-FOS Anti-c-Fos antibody [EPR21930-238] ab222699 was used with the 3D Tissue Staining Kit – CUBIC (ab316248).
White: nuclear staining, Yellow: c-FOS.
The sample was imaged using a light-sheet microscope.
IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (ab316248)
Iba1 immunohistochemistry staining of whole mouse brain using rabbit anti-Iba1 antibody
Anti-Iba1 Anti-Iba1 antibody [EPR16588] ab178846 was used with Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.
For a whole mouse brain we recommend starting with 3.5 ug of Anti-Iba1 antibody [EPR16588] ab178846 and using a Fab fragment secondary antibody with 2.34 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.
The sample was imaged using a light-sheet microscope.
IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (ab316248)
NeuN immunohistochemistry staining of whole mouse brain using rabbit anti-NeuN antibody
Anti-NeuN Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was used with Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.White: nuclear staining
Red: NeuN.Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample. For a whole mouse brain we recommend starting with 10 ug of Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.
The sample was imaged using a light-sheet microscope.
IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (ab316248)
After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246), anti-CD31 Anti-CD31 antibody [EPR17260-254] ab213175 was used with the 3D Tissue Staining Kit – CUBIC (ab316248).
The color in the image represents the depth of the signal.
The sample was imaged using a light-sheet microscope .
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