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AB316248

3D Tissue Staining Kit – CUBIC

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(5 Publications)

3D Tissue Staining Kit – CUBIC (ab316248) is a simple, easy-to-use kit for rapid tissue clearing for 3D imaging using standard laboratory equipment. Suitable for Mouse organs.

- Compatible with Tissue Clearing Kit – CUBIC (ab316246) reagents
- Optimized for PFA fixed tissues
- Suitable for light-sheet microscopy, confocal laser scanning microscopy and two-photon microscopy
4 Images
IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)

After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (ab316246), anti-CD31 ab213175 was used with the 3D Tissue Staining Kit – CUBIC (ab316248).

The color in the image represents the depth of the signal.

The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)

After clearing the whole mouse brain with the Tissue clearing kit – CUBIC (ab316246), anti-c-FOS ab222699 was used with the 3D Tissue Staining Kit – CUBIC (ab316248).

White : nuclear staining, Yellow : c-FOS.

The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)

Anti-Iba1 ab178846 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.

Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.

For a whole mouse brain we recommend starting with 3.5 ug of ab178846 and using a Fab fragment secondary antibody with 2.34 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.

The sample was imaged using a light-sheet microscope.

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)
  • IHC-tissue clearing

Supplier Data

IHC-tissue clearing - 3D Tissue Staining Kit – CUBIC (AB316248)

Anti-NeuN ab177487 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate stain and clear a whole mouse brain.

White : nuclear staining
Red : NeuN.

Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample. For a whole mouse brain we recommend starting with 10 ug of ab177487 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.

The sample was imaged using a light-sheet microscope.

Key facts

Reacts with

Mouse

Assay Platform

Reagents

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Reactivity data

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Product details

3D Tissue Staining Kit – CUBIC (ab316248) enables the easy set-up of 3D tissue imaging using simple protocols and standard laboratory equipment.

Together with the Tissue Clearing Kit – CUBIC (ab316246) we provide a versatile tissue clearing and 3D tissue staining method that allows the observation of all cells in organs and the body. The kits are designed for safe and easy use in the lab.

  • Practical application of a special tissue staining process that uniformly stains 3D blocks of tissue
  • Highly reproducible protocol compatible with various nuclear and antibody stains

The provided protocol solves issues pertaining to safety, waste processing, microscope compatibility, fluorescent protein signal retainability, etc.

To help you get started with tissue clearing, please visit the available data on tested antibodies on this datasheet. Validated antibodies are for key markers and are tested for tissue clearing and staining with whole mouse brains. If you wish to set up tissue clearing with antibodies that we haven’t tested yet, please consult the protocol book for further details.

To learn more about tissue clearing, and tissue clearing methods (including for 3D cell cultures), please review our guide to tissue clearing.

The tissue staining kit uses CUBIC technology.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Product protocols

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Target data

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 14:3506-3537 PubMed31748753

2019

Advanced CUBIC tissue clearing for whole-organ cell profiling.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Katsuhiko Matsumoto,Tomoki T Mitani,Shuhei A Horiguchi,Junichi Kaneshiro,Tatsuya C Murakami,Tomoyuki Mano,Hiroshi Fujishima,Ayumu Konno,Tomonobu M Watanabe,Hirokazu Hirai,Hiroki R Ueda

Cell reports 24:2196-2210.e9 PubMed30134179

2018

Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Kazuki Tainaka,Tatsuya C Murakami,Etsuo A Susaki,Chika Shimizu,Rie Saito,Kei Takahashi,Akiko Hayashi-Takagi,Hiroshi Sekiya,Yasunobu Arima,Satoshi Nojima,Masako Ikemura,Tetsuo Ushiku,Yoshihiro Shimizu,Masaaki Murakami,Kenji F Tanaka,Masamitsu Iino,Haruo Kasai,Toshikuni Sasaoka,Kazuto Kobayashi,Kohei Miyazono,Eiichi Morii,Tadashi Isa,Masashi Fukayama,Akiyoshi Kakita,Hiroki R Ueda

Scientific reports 7:9269 PubMed28839164

2017

CUBIC pathology: three-dimensional imaging for pathological diagnosis.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Satoshi Nojima,Etsuo A Susaki,Kyotaro Yoshida,Hiroyoshi Takemoto,Naoto Tsujimura,Shohei Iijima,Ko Takachi,Yujiro Nakahara,Shinichiro Tahara,Kenji Ohshima,Masako Kurashige,Yumiko Hori,Naoki Wada,Jun-Ichiro Ikeda,Atsushi Kumanogoh,Eiichi Morii,Hiroki R Ueda

Cell reports 20:236-250 PubMed28683317

2017

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Shimpei I Kubota,Kei Takahashi,Jun Nishida,Yasuyuki Morishita,Shogo Ehata,Kazuki Tainaka,Kohei Miyazono,Hiroki R Ueda

Cell 157:726-39 PubMed24746791

2014

Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.

Applications

IHC-tissue clearing

Species

Unspecified reactive species

Etsuo A Susaki,Kazuki Tainaka,Dimitri Perrin,Fumiaki Kishino,Takehiro Tawara,Tomonobu M Watanabe,Chihiro Yokoyama,Hirotaka Onoe,Megumi Eguchi,Shun Yamaguchi,Takaya Abe,Hiroshi Kiyonari,Yoshihiro Shimizu,Atsushi Miyawaki,Hideo Yokota,Hiroki R Ueda
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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