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AB287843

3T3-L1 Differentiation Kit

5

(1 Review)

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(1 Publication)

3T3-L1 Differentiation Kit (ab287843) provides enough supplements to make 100 ml of differentiation medium & 600 ml of maintenance medium. Sufficient material for 12x 100 mm culture dishes.

- Use to differentiate 3T3-L1 preadipocytes to adipocytes
- Suitable for obesity, adipogenesis, lipolysis and lipid metabolism assays
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Functional Studies - 3T3-L1 Differentiation Kit (AB287843)
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Supplier Data

Functional Studies - 3T3-L1 Differentiation Kit (AB287843)

(a) 3T3-L1 cells before differentiation.

(b) 3T3-L1 cells 7 days after the initiation of differentiation and stained with neutral lipid dye Oil Red O.

(c) Triglyceride levels in preadipocytes grown in a 96-well cell culture plate and in adipocytes 7 days after differentiation.

Key facts

Sample types

3T3-L1 cells, Primary preadipocytes

Assay type

Cell-based

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Product details

This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.

The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin, 1 μM dexamethasone, 500 μM IBMX, and 1 μM rosiglitazone in the differentiation media.

Check out our range of cell differeniation biochemcials here

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100mL": { "sellingSize": "100 mL", "publicAssetCode":"ab287843-100mL", "assetComponentDetails": [ { "size":"1 x 0.6 mL", "name":"Insulin", "number":"AB287843-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1000X Differentiation Cocktail", "number":"AB287843-CMP02", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"DMSO I", "number":"AB287843-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 12:e70089 PubMed39435735

2024

Pde3a and Pde3b regulation of murine pulmonary artery smooth muscle cell growth and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina N Krause,Gabrielle McGeorge,Jennifer L McPeek,Sidra Khalid,Leif D Nelin,Yusen Liu,Bernadette Chen
View all publications
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Product promise

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