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This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin insulin.


Images

Functional Studies - 3T3-L1 Differentiation Kit (AB287843), expandable thumbnail

Key facts

Sample types

Cell culture supernatant, Tissue

Assay type

Cell-based

What's included?

100 mL
Components
1000X Differentiation Cocktail
1 x 1 Vial
DMSO I
1 x 0.5 mL
Insulin
1 x 0.6 mL

Recommended products

This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin insulin.

Key facts

Sample types

Cell culture supernatant, Tissue

Assay type

Cell-based

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin, 1 μM dexamethasone, 500 μM IBMX, and 1 μM rosiglitazone in the differentiation media.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - 3T3-L1 Differentiation Kit (ab287843), expandable thumbnail

    Functional Studies - 3T3-L1 Differentiation Kit (ab287843)

    (a) 3T3-L1 cells before differentiation.

    (b) 3T3-L1 cells 7 days after the initiation of differentiation and stained with neutral lipid dye Oil Red O.

    (c) Triglyceride levels in preadipocytes grown in a 96-well cell culture plate and in adipocytes 7 days after differentiation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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