This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin insulin.
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Key facts
- Sample types
- 3T3-L1 cells, Primary preadipocytes
- Assay type
- Cell-based
What's included?
Recommended products
This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin insulin.
Key facts
- Sample types
- 3T3-L1 cells, Primary preadipocytes
- Assay type
- Cell-based
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
This 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 μg/ml insulin, 1 μM dexamethasone, 500 μM IBMX, and 1 μM rosiglitazone in the differentiation media.
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1 product image
Functional Studies - 3T3-L1 Differentiation Kit (ab287843)
(a) 3T3-L1 cells before differentiation.
(b) 3T3-L1 cells 7 days after the initiation of differentiation and stained with neutral lipid dye Oil Red O.
(c) Triglyceride levels in preadipocytes grown in a 96-well cell culture plate and in adipocytes 7 days after differentiation.
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