6-Phosphogluconate Dehydrogenase Assay Kit (ab241016) provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples.
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- Open life sciences 18:2022051420236-Phosphogluconate dehydrogenase inhibition arrests growth and induces apoptosis in gastric cancer via AMPK activation and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Chen,Pan Du,Zhenguo Zhang,Di BaoPubMed 36852400
- Oncogene 42:613-6242022PCNA regulates primary metabolism by scaffolding metabolic enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa M Røst,Synnøve B Ræder,Camilla Olaisen,Caroline K Søgaard,Animesh Sharma,Per Bruheim,Marit OtterleiPubMed 36564470
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Tissue Homogenate, Adherent cells
Target data
Function
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
Alternative names
PGDH, PGD
What's included?
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6-Phosphogluconate Dehydrogenase Assay Kit (ab241016) provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples.
Alternative names
PGDH, PGD
Key facts
- Detection method
Colorimetric
- Sample types
Suspension cells, Tissue Homogenate, Adherent cells
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
6-Phosphogluconate Dehydrogenase Assay Kit (ab241016) provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples.
In this assay, 6-PGD converts 6-phosphogluconate into an intermediate, generating NADPH, which subsequently reduces a colorless probe into a strongly colored product detectable by absorbance at 460 nm.
The assay is high- throughput adaptable and can detect less than 0.05 mU of 6-PGD activity.
This product is manufactured by BioVision, an Abcam company and was previously called K540 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit. K540-100 is the same size as the 100 test size of ab241016.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.
- Biological function summary
The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.
- Pathways
PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.
- Associated diseases and disorders
Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.
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3 product images
Functional Studies - 6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
NADPH standard curve.
Functional Studies - 6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
Reaction kinetics of 6-Phosphogluconate dehydrogenase activity in positive control, rat liver (66 μg protein) and rat spleen (56 μg protein) using appropriate background controls.
Functional Studies - 6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
6-Phosphogluconate dehydrogenase specific activity calculated in rat liver and spleen tissue lysates. Assays were performed following the kit protocol.
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