7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml).
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 5 µL for 106 Cells | Notes Incubate for 5 minutes at room temperature before analysis. |
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7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml).
7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of non-viable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Exitation = 540 nm / emission = 645 nm.
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