ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703) uses an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore.
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- International journal of radiation oncology, biology, physics 113:177-1912022Pharmacologic ACE-Inhibition Mitigates Radiation-Induced Pneumonitis by Suppressing ACE-Expressing Lung Myeloid Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuru Prasad Sharma,Brian L Fish,Anne C Frei,Jayashree Narayanan,Tracy Gasperetti,Dana Scholler,Lauren Pierce,Nathan Szalewski,Noah Blue,Meetha Medhora,Heather A HimburgPubMed 35093482
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Cell culture extracts, Tissue, Serum
- Reactive species
Mammals
Target data
Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Targets
ACE2
Alternative names
Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, DCP1, DCP, ACE
What's included?
Recommended products
ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703) uses an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore.
Alternative names
Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, DCP1, DCP, ACE
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Cell culture extracts, Tissue, Serum
- Reactive species
Mammals
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703) utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples, such as lung, heart and kidney tissue, and serum and plasma. It can also be used to determine the enzymatic activity of purified ACE1.
This product is manufactured by BioVision, an Abcam company and was previously called K227 Angiotensin I Converting Enzyme Activity (ACE1) Assay Kit (Fluorometric). K227-100 is the same size as the 100 test size of ab239703.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiotensin Converting Enzyme 1 also known as ACE1 or ACE is an important enzyme in the renin-angiotensin system. This enzyme exhibits a significant role in blood pressure regulation. ACE1 is a zinc-metallopeptidase with a molecular weight of approximately 130 kDa. It converts angiotensin I into the potent vasoconstrictor angiotensin II a critical function in cardiovascular physiology. ACE1 is widely expressed in endothelial cells particularly in the lungs kidneys and the small intestine.
- Biological function summary
The enzyme generates angiotensin II by cleaving angiotensin I. Angiotensin II an important effector peptide impacts cardiovascular and renal systems influencing vasoconstriction and fluid balance. While not directly forming a complex ACE1's activity increases the potency of angiotensin II which binds to angiotensin II receptors to exert its effects therefore indirectly forming a functional signaling complex.
- Pathways
ACE1 plays a central role in the renin-angiotensin system and the kallikrein-kinin system. The enzyme's activity boosts angiotensin II production which connects it to the regulation of blood pressure via the renin-angiotensin pathway. ACE1 also indirectly interacts with proteins like bradykinin by degrading them modulating kinin-related functions and influencing inflammation and tension in vascular smooth muscle.
- Associated diseases and disorders
Understanding ACE1 is important for addressing hypertension and congestive heart failure. ACE1's conversion of angiotensin I to angiotensin II means overactivity can cause elevated blood pressure leading to hypertension. This makes ACE inhibitors such as lisinopril and ramipril therapeutic for these conditions. Furthermore its connection with aldosterone production places ACE1 in relevance to heart failure as excessive aldosterone can cause detrimental remodeling of cardiac tissue.
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3 product images
Cellular Activity - ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703)
Abz-Standard Curve (0-1000 pmol), error bars indicate SD (n=3).
Cellular Activity - ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703)
Kinetic activity curves using different amounts of ACE1. Positive Control in the assay.
Cellular Activity - ACE Assay Kit (Angiotensin I Converting Enzyme) (ab239703)
ACE1 activity was measured for different types of rat tissue samples (lung, heart and kidney; 0.6 µg each).
ACE1 activity was measured for different types of rat tissue samples (lung, heart and kidney; 0.6 μg each). Assays were performed following the kit protocol.
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