Acetate Assay Kit (Colorimetric)
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(13 Publications)
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Supplier Data
Functional Studies - Acetate Assay Kit (Colorimetric) (AB204719)
Measurement of Acetate in human serum (5 μl), fish liver (~100 μg) and egg (~200 μg).
- FuncS
Supplier Data
Functional Studies - Acetate Assay Kit (Colorimetric) (AB204719)
Acetate Standard Curve.
- Schematic Diagram
Supplier Data
Schematic Diagram - Acetate Assay Kit (Colorimetric) (AB204719)
Representative image of Acetate Assay Kit (Colorimetric) ab204719
Components shown from left to right :
- Acetate Substrate Mix
- Developer Solution III
- Acetate Standard
- ATP II
- Acetate Enzyme Mix
- Acetate Assay Buffer
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
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Product details
How the assay works
In Acetate Assay Kit (Colorimetric) protocol, free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm.
Acetate assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Incubate for 40 min
- Analyze with microplate reader
How other researchers are using
Acetate Assay Kit (Colorimetric) has been used in a variety of sample type including:
- E. coli and Enterobacter supernatant 1
- Mouse spinal cord and serum samples 2
- Human Esophageal squamous cell carcinoma cell lines 3
References:
1-Estrela S al. 2022
2-Jin S et al. 2021
3-Odera J et al. 2020
This product is manufactured by BioVision, an Abcam company and was previously called K658 Acetate Colorimetric Assay Kit. K658-100 is the same size as the 100 test size of ab204719.
Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Many metabolic processes utilize acetate serving as an important building block in the synthesis of fatty acids and cholesterol. It is not typically associated with a complex but it collaborates with coenzymes and enzymes to drive biosynthetic reactions. For instance acetate acts as a precursor in the formation of acetyl-CoA an essential molecule in energy production and lipid synthesis. The convergence of acetate in such biochemical processes highlights its importance in maintaining cellular energy balance and material synthesis.
Pathways
Acetate prominently features in the acetyl-CoA biosynthesis pathway an important conduit for energy production. This pathway integrates acetate into cycles like the citric acid cycle where it partners with enzymes like citrate synthase. Another significant connection exists with the lipid biosynthesis pathway where acetate is converted into fatty acids relating closely with acetyl-CoA carboxylase. These pathways highlight the centrality of acetate in both energy provision and cell membrane synthesis affirming its integral role in cell metabolism.
Product protocols
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Target data
Publications (13)
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Cell systems 13:29-42.e7 PubMed34653368
2021
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British journal of anaesthesia 127:296-309 PubMed33934892
2021
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Cell biology international 45:1278-1287 PubMed33559958
2021
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The Biochemical journal 477:3075-3089 PubMed32776152
2020
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Cell reports 31:107722 PubMed32492430
2020
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Cell metabolism 29:886-900.e5 PubMed30661930
2019
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Scientific reports 8:7404 PubMed29743535
2018
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European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 28:211-224 PubMed29174530
2017
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FEBS open bio 7:1932-1942 PubMed29226080
2017
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Cell 170:664-677.e11 PubMed28802039
2017
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com