AB180875

Acetoacetate Assay Kit (Colorimetric)

Name: Acetoacetate Assay Kit (Colorimetric) (ab180875) | Abcam
Brand: Abcam Limited
SKU: AB180875
Price: 845 USD
Availability: InStock

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(12 Publications)

Acetoacetate Assay Kit (Colorimetric) ab180875 uses the sodium nitroferricyanide assay method to quantify acetoacetate levels. Readout on any colorimetric (550 nm) plate reader.

6 Images

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

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Supplier Data

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Measurement of Acetoacetate in urine.

Samples were deproteinized by spin filtering. 100 μL of undiluted urine was spiked with known amounts of AcAc (30 nmol).

Assays were performed following the kit protocol.

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Supplier Data

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Standard Curve for equimolar amounts of Acetoacetate, β-HB and Acetone (4°C, 100 min.). The other two ketone bodies β-hydroxybutyrate or Acetone are not detected in the assay due to much lower sensitivity (β-HB) and much lower concentration (Acetone).

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Lab

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional assay - 180875 Acetoacetate Assay Kit (Colorimetric).

Colorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted.

FuncS

Supplier Data

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Time course experiment.

Time course of color development at 4°C and 25°C (100 nmol Acetoacetate).

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Lab

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional assay - 180875 Acetoacetate Assay Kit (Colorimetric).

Acetoacetate measured in urine samples showing concentration (micromolar).

Schematic Diagram - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Schematic Diagram

Supplier Data

Schematic Diagram - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Representative image of Acetoacetate Assay Kit (Colorimetric) ab180875

Components shown from left to right :

- Acetoacetate Substrate

- Acetoacetate Standard

- Acetoacetate Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Other biological fluids

Assay type

Quantitative

Sensitivity

< 25 µM

Assay time

25m

Assay Platform

Microplate reader

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Product details

Acetoacetate Assay Kit (Colorimetric) ab180875 adapts the traditional qualitative assay for acetoacetate using dipsticks, and sodium nitroferricyanide as a chromophore, to provide an assay optimized for use with plate readers that can be used to quantitate endogenous levels of AcAc in human urine, plasma and other biological fluids.

Acetoacetate assay principle
In the nitroferricyanide method acetoacetate assay, AcAc reacts with sodium nitroferricyanide to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 μM.

Acetoacetate assay protocol summary
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode for 10-15 min

Related and recommended products
Acetoacetate (AcAc), a β-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-β-hydroxybutyrate (β-HB), or decarboxylated producing acetone ((CH₃)₂CO). Ketone bodies [βHB: 78%; AcAc: 20% & (CH₃)₂CO: 2%] are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore

This assay kit is used to measure the acetoacetate element of ketone bodies.

Use beta Hydroxybutyrate (beta HB) Assay Kit ab83390 to measure the beta hydroxybutyrate element of ketone bodies.

To measure both the beta hydroxybutyrate and acetoacetate elements of ketone bodies in a single assay kit, use Ketone Body Assay Kit ab318308.

Other notes
This product was previously called K650 Biovision Acetoacetate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab180875-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Acetoacetate Standard", "number":"AB180875-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Acetoacetate Assay Buffer", "number":"AB180875-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"Acetoacetate Substrate", "number":"AB180875-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetoacetate is a four-carbon ketone body that plays a central role in energy metabolism particularly during periods of fasting or carbohydrate restriction. It has a molecular mass of 102.09 g/mol. Acetoacetate is mainly produced in the liver's mitochondria and can be converted to either acetone or beta-hydroxybutyrate another key ketone body dependent on the enzymatic conditions present. This conversion process involves several enzymes including acetoacetate decarboxylase for acetoacetate to acetone and beta-hydroxybutyrate dehydrogenase for acetoacetate to beta-hydroxybutyrate.

Biological function summary

The presence of acetoacetate serves as an alternative energy source for many tissues including the brain heart and muscles when glucose availability is low. Acetoacetate and its conversion to beta-hydroxybutyrate are important during states of low-carbohydrate intake prolonged exercise or starvation. These compounds bypass the blood-brain barrier to supply energy to neurons. Acetoacetate itself does not form part of a larger protein complex but interacts directly with enzymes to mediate its functions.

Pathways

Acetoacetate is predominantly involved in the ketogenic pathway where it is synthesized from the breakdown of fatty acids. This pathway plays an important role in maintaining energy balance during carbohydrate scarcity. In the citric acid cycle acetoacetate is linked to acetyl-CoA aiding in the generation of ATP. Proteins like succinyl-CoA:3-ketoacid CoA transferase facilitate acetoacetate's conversion into energy-yielding substrates interrelating with both pathways for effective energy modulation.

Abnormal levels of acetoacetate can relate to conditions such as diabetic ketoacidosis and alcoholic ketoacidosis. In diabetic ketoacidosis elevated acetoacetate levels are due to insulin deficiency leading to excessive ketone body production often accompanied by high beta-hydroxybutyrate levels. This condition links acetoacetate to insulin a critical regulatory hormone. In alcoholic ketoacidosis chronic alcohol consumption results in acetoacetate accumulation due to disrupted carbohydrate metabolism highlighting its role in metabolic imbalances.

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Product protocols

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Target data

See full target information Acetoacetate

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, obesity & metabolism 26:3137-3146 PubMed38699792

2024

Pioglitazone reduces serum ketone bodies in sodium-glucose cotransporter-2 inhibitor-treated non-obese type 2 diabetes: A single-centre, randomized, crossover trial.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yang,Han Yue,Qinqin Xu,Shiying Shao,Yong Chen

Journal of cachexia, sarcopenia and muscle 15:975-988 PubMed38632714

2024

Hepatic signal transducer and activator of transcription-3 signalling drives early-stage pancreatic cancer cachexia via suppressed ketogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Paige C Arneson-Wissink,Heike Mendez,Katherine Pelz,Jessica Dickie,Alexandra Q Bartlett,Beth L Worley,Stephanie M Krasnow,Robert Eil,Aaron J Grossberg

Nanomaterials (Basel, Switzerland) 12: PubMed35159714

2022

Punicalagin and Ketogenic Amino Acids Loaded Organic Lipid Carriers Enhance the Bioavailability, Mitochondrial β-Oxidation, and Ketogenesis in Maturing Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pandurangan Subash-Babu,Nouf Al-Numair,Tahani Almuzaini,Jegan Athinarayanan,Ali Abdullah Alshatwi

FEBS open bio 11:1893-1906 PubMed33728819

2021

The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Zuyin Li,Weixing Shen,Gang Wu,Changjiang Qin,Yijie Zhang,Yupeng Wang,Guohe Song,Chao Xiao,Xin Zhang,Guilong Deng,Ruitao Wang,Xiaoliang Wang

Journal of the American Heart Association 9:e013039 PubMed32750298

2020

Increased Glucose Availability Attenuates Myocardial Ketone Body Utilization.

Applications

Unspecified application

Species

Unspecified reactive species

Manoja K Brahma,Chae-Myeong Ha,Mark E Pepin,Sobuj Mia,Zhihuan Sun,John C Chatham,Kirk M Habegger,Evan Dale Abel,Andrew J Paterson,Martin E Young,Adam R Wende

GeroScience 42:667-686 PubMed31975053

2020

Short-term time-restricted feeding is safe and feasible in non-obese healthy midlife and older adults.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher R Martens,Matthew J Rossman,Melissa R Mazzo,Lindsey R Jankowski,Erzsebet E Nagy,Blair A Denman,James J Richey,Sarah A Johnson,Brian P Ziemba,Yang Wang,Courtney M Peterson,Michel Chonchol,Douglas R Seals

Diagnostics (Basel, Switzerland) 9: PubMed31861135

2019

Blood Ketone Bodies and Breath Acetone Analysis and Their Correlations in Type 2 Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Valentine Saasa,Mervyn Beukes,Yolandy Lemmer,Bonex Mwakikunga

Stroke 49:2173-2181 PubMed30354983

2018

Imaging the Effects of β-Hydroxybutyrate on Peri-Infarct Neurovascular Function and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Bazzigaluppi,Evelyn M Lake,Tina L Beckett,Margaret M Koletar,Iliya Weisspapir,Stefan Heinen,James Mester,Aaron Lai,Rafal Janik,Adrienne Dorr,JoAnne McLaurin,Greg J Stanisz,Peter L Carlen,Bojana Stefanovic

Translational psychiatry 8:66 PubMed29540664

2018

Prebiotic attenuation of olanzapine-induced weight gain in rats: analysis of central and peripheral biomarkers and gut microbiota.

Applications

FuncS

Species

Unspecified reactive species

Amy Chia-Ching Kao,Sonia Spitzer,Daniel C Anthony,Belinda Lennox,Philip W J Burnet

Frontiers in physiology 8:806 PubMed29109686

2017

Ketone Diester Ingestion Impairs Time-Trial Performance in Professional Cyclists.

Applications

Unspecified application

Species

Unspecified reactive species

Jill J Leckey,Megan L Ross,Marc Quod,John A Hawley,Louise M Burke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Acetoacetate Assay Kit (Colorimetric)

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Functional Studies - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Schematic Diagram - Acetoacetate Assay Kit (Colorimetric) (AB180875)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay time

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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