Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.
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Publications
- Nature communications 14:76392023Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun HuangPubMed 37993431
- Gerontology 69:1424-14362023Long-Term Acetylcholinesterase Depletion Alters the Levels of Key Synaptic Proteins while Maintaining Neuronal Markers in the Aging Zebrafish (Danio rerio) Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElif Tugce Karoglu-Eravsar,Melek Umay Tuz-Sasik,Aysenur Karaduman,Ayse Gokce Keskus,Ayca Arslan-Ergul,Ozlen Konu,Hulusi Kafaligonul,Michelle M AdamsPubMed 37793352
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Plasma, Cell culture extracts, Serum, Cell Lysate
- Assay type
Enzyme activity
- Assay time
30m
- Sensitivity
= 1 mU/mL
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Target data
Function
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Alternative names
Acetylcholinesterase, AChE, ACHE
What's included?
Recommended products
Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.
Alternative names
Acetylcholinesterase, AChE, ACHE
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Plasma, Cell culture extracts, Serum, Cell Lysate
- Assay type
Enzyme activity
- Assay time
30m
- Assay Platform
Microplate reader
- Sensitivity
= 1 mU/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.
The acetylcholinesterase assay protocol uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The absorption intensity of DTNB adduct (410 nm) is used to measure the amount of thiocholine formed, which is proportional to the AChE activity.
The assay will detect as little as 0.1 mU AChE in a 100 μL assay volume (1 mU/mL).
Acetylcholinesterase assay protocol summary:
- add standards and samples to wells
- add acetylthiocholine reaction mix and incubate for 10-30 min at room temp
- analyze with a microplate reader
Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.
If you are looking for a fluorometric kit, we recommend Acetylcholinesterase Assay Kit (Fluorometric-Green) (ab133872) (Ex/Em = 490/520 nm) or Acetylcholinesterase Assay Kit (Fluorometric-Red) (ab133873) (Ex/Em = 540/590 nm).
Supplementary info
- Activity summary
Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.
- Biological function summary
Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.
- Pathways
Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.
- Associated diseases and disorders
Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.
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5 product images
Morzelle M et al., PLoS One, 11(11). Fig 6. doi: 10.1371/journal.pone.0166123 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
ab138871 was used to determine AChE activity in the cortex and in the hippocampus. AChE activity was measured at 410 nm.
Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
AChE measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample
Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
AChE measured in cell lysates showing quantity (mU) per 1 mln of tested cells
Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
AChE measured in biological fluids showing quantity (mU) per mL of tested sample
Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
Sample Standard Curve for Acetylcholinesterase. Acetylcholinesterase dose response was measured in a white/clear bottom 96-well plate with ab138871 Acetylcholinesterase Assay Kit (Colorimetric) using a microplate reader. As low as 0.1 mU/well of acetylcholinesterase can be detected with 30 minutes incubation (n=3)
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