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AB138871

Acetylcholinesterase Assay Kit (Colorimetric)

5

(3 Reviews)

|

(72 Publications)

Acetylcholinesterase Assay Kit ab138871 uses DTNB to quantify thiocholine produced by the action of acetylcholinesterase / AChE activity. Readout on any colorimetric (410nm) plate reader.

- Measure AChE activity in 30 minutes
- Cited in >60 publications

View Alternative Names

Acetylcholinesterase, AChE, ACHE

5 Images
Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)
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PubMed

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)

ab138871 was used to determine AChE activity in the cortex and in the hippocampus. AChE activity was measured at 410 nm.

Morzelle M et al., PLoS One, 11(11). Fig 6. doi: 10.1371/journal.pone.0166123 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)
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Lab

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)

AChE measured in cell lysates showing quantity (mU) per 1 mln of tested cells

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)
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Supplier Data

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)

Sample Standard Curve for Acetylcholinesterase. Acetylcholinesterase dose response was measured in a white/clear bottom 96-well plate with ab138871 Acetylcholinesterase Assay Kit (Colorimetric) using a microplate reader. As low as 0.1 mU/well of acetylcholinesterase can be detected with 30 minutes incubation (n=3)

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)
  • FuncS

Lab

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)

AChE measured in biological fluids showing quantity (mU) per mL of tested sample

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)
  • FuncS

Lab

Functional Studies - Acetylcholinesterase Assay Kit (Colorimetric) (AB138871)

AChE measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Plasma, Cell culture extracts, Serum, Cell Lysate

Assay type

Enzyme activity

Sensitivity

= 1 mU/mL

Assay time

30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.

How the assay works
The acetylcholinesterase assay protocol uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The absorption intensity of DTNB adduct (410 nm) is used to measure the amount of thiocholine formed, which is proportional to the AChE activity.

The assay will detect as little as 0.1 mU AChE in a 100 μL assay volume (1 mU/mL).

Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.

Acetylcholinesterase assay protocol summary
- add standards and samples to wells
- add acetylthiocholine reaction mix and incubate for 10-30 min at room temp
- analyze with a microplate reader

Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.

Alternative assay kits
For fluorometric detection, we recommend Acetylcholinesterase Assay Kit (Fluorometric-Green) (ab133872) (Ex/Em = 490/520 nm) or Acetylcholinesterase Assay Kit (Fluorometric-Red) (ab133873) (Ex/Em = 540/590 nm).

How other researchers are using ab138871
ab138871 has been used with a variety of sample types including:
- Human derived mesenchymal stem cell line 1
- Mouse colon tissue 2
- Zebrafish Brain tissues
References: 1 - He Y t al. 2023; 2 - Kim NY et al. 2023; 3 - Karoglu-Eravsar E at al. 2023.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab138871-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Acetylthiocholine", "number":"AB138871-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer", "number":"AB138871-CMP03", "productcode":"" }, { "size":"1 x 5 Unit", "name":"Acetylcholinesterase Standard", "number":"AB138871-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"DTNB", "number":"AB138871-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.
Biological function summary

Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.

Pathways

Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.

Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme's interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.
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Product protocols

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Target data

Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
See full target information ACHE
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Publications (72)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed40116124

2025

Water extract of improves age‑related cognitive decline by inhibiting acetylcholinesterase activity and the acetylcholine signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Eun Kim,Kyeong-Seon Min,Jun Go,Hye-Yeon Park,Young-Keun Choi,In-Bok Lee,Jaewon Shin,Hyun-Ju Cho,Hong-Sik Kim,Dae Youn Hwang,Won-Keun Oh,Kyoung-Shim Kim,Chul-Ho Lee

Lab on a chip 25:1854-1874 PubMed40052475

2025

A gut-brain axis on-a-chip platform for drug testing challenged with donepezil.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Fanizza,Simone Perottoni,Lucia Boeri,Francesca Donnaloja,Francesca Negro,Francesca Pugli,Gianluigi Forloni,Carmen Giordano,Diego Albani

Heliyon 11:e42673 PubMed40034321

2025

Endogenous extraction yielded high quality sulforaphane from broccoli sprouts unveils potent antioxidant and anti-Alzheimer's activities.

Applications

Unspecified application

Species

Unspecified reactive species

Shyam Kokkattunivarthil Uthaman,Wan Seok Kang,Ju-Young Park,Sunoh Kim,Duc Dat Le,Suk-Jung Oh,Karthik Murugesh,Laura Minju Oh,Mina Lee,Jin Woo Park

RSC advances 14:40267-40286 PubMed39717818

2024

Cognitive-enhancing effect of fruit on scopolamine-induced cognitive impairment in rats: metabolite profiling, , and investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Hagar M Hussein,Mostafa A Abdel Kawy,Basma M Eltanany,Laura Pont,Fernando Benavente,Ahmed M Fayez,Radwan Alnajjar,Ahmed A Al-Karmalawy,Azza R Abdelmonem,Engy Mohsen

Chinese medicine 19:166 PubMed39605070

2024

Melittin - the main component of bee venom: a promising therapeutic agent for neuroprotection through keap1/Nrf2/HO-1 pathway activation.

Applications

Unspecified application

Species

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Cong Duc Nguyen,Jaehee Yoo,Sang Jun Jeong,Hai-Anh Ha,Ji Hye Yang,Gihyun Lee,Jeong Cheol Shin,Jae-Hong Kim

Cellular and molecular neurobiology 44:70 PubMed39441380

2024

Metformin Mitigates Trimethyltin-Induced Cognition Impairment and Hippocampal Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mahdieh Taheri,Mehrdad Roghani,Reza Sedaghat

Molecules (Basel, Switzerland) 29: PubMed39339437

2024

Synthesis, Characterization, and Biological Evaluation of -Methyl Derivatives of Norbelladine.

Applications

Unspecified application

Species

Unspecified reactive species

S Mahsa Hashemian,Natacha Merindol,Alexis Paquin,Amita Singh,Lionel Berthoux,Benoit Daoust,Isabel Desgagné-Penix

Biomedical reports 21:130 PubMed39070112

2024

Alleviative effect of scopolamine‑induced memory deficit via enhancing antioxidant and cholinergic function in rats by pinostrobin from (L.).

Applications

Unspecified application

Species

Unspecified reactive species

Sitthisak Thongrong,Tichanon Promsrisuk,Napatr Sriraksa,Serm Surapinit,Jinatta Jittiwat,Ratchaniporn Kongsui

Neurochemical research 49:2803-2820 PubMed38987448

2024

Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Reham L Younis,Rehab M El-Gohary,Asmaa A Ghalwash,Islam Ibrahim Hegab,Maram M Ghabrial,Azza M Aboshanady,Raghad A Mostafa,Alaa H Abd El-Azeem,Eman E Farghal,Asmaa A E Belal,Haidy Khattab

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 49:1916-1924 PubMed38956176

2024

Distinct synaptic mechanisms drive the behavioral response to acute stress and rapid correction by ketamine.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Woon Kim,Benjamin Kleinfelter,Ege T Kavalali,Lisa M Monteggia
View all publications
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