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AB138873

Acetylcholinesterase Assay Kit (Fluorometric -Red)

5

(1 Review)

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(3 Publications)

Acetylcholinesterase Assay Kit (Fluorometric -Red) ( ab138873) uses a red indicator to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions.

View Alternative Names

Acetylcholinesterase, AChE, ACHE

3 Images
Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)
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Lab

Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)

AChE measured in cell lysates showing activity per 1 million cells

Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)
  • FuncS

Lab

Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)

AChE measured in various species showing activity per mL sample tested

Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)
  • FuncS

Supplier Data

Functional Studies - Acetylcholinesterase Assay Kit (Fluorometric -Red) (AB138873)

Sample Standard Curve for Acetylcholinesterase. Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138873 using a fluorescence microplate reader. As low as 0.01 mU/well (0.1 mU/ml) acetylcholinesterase can be detected with 20 minutes incubation (n=3).

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Plasma, Serum, Cell Lysate

Assay type

Enzyme activity

Sensitivity

= 0.1 mU/mL

Assay time

20m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Acetylcholinesterase Assay Kit (Fluorometric -Red) ( ab138873) uses a red indicator to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions.

The assay can be used for monitoring and quantifying AChE activity in blood, cell extracts or other solutions. The fluorescence intensity of the indicator is proportional to the amount of choline formed, which is proportional to the AChE activity.

The assay is an optimized "mix and read" assay using a simple one-step fluorometric protocol to detect as little as 0.01 mU AChE in a 100 μl assay volume (0.1 mU/ml). The assay can be performed in a 96-well or 384-well microtiter-plate format, and can be easily read with a fluorescence microplate reader at Ex/Em = 540/590 nm or with an absorbance microplate reader at OD = 575 nm.

Acetylcholinesterase assay protocol summary:
- add samples and standards to wells
- add assay mixture and incubate for 10-30 min
- analyze with microplate reader

Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.

If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure green fluorescnce (Ex/Em = 490/520 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872).

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.
Biological function summary

Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.

Pathways

Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.

Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.
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Product protocols

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Target data

Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
See full target information ACHE
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:20329 PubMed36434021

2022

Succinate prodrugs in combination with atropine and pralidoxime protect cerebral mitochondrial function in a rodent model of acute organophosphate poisoning.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Piel,Joanna I Janowska,J Laurenson Ward,Meagan J McManus,Joshua S Jose,Jonathan Starr,Malkah Sheldon,Carly L Clayman,Eskil Elmér,Magnus J Hansson,David H Jang,Michael Karlsson,Johannes K Ehinger,Todd J Kilbaugh

Environmental health perspectives 129:77001 PubMed34259569

2021

Gene-Environment Interactions in Developmental Neurotoxicity: a Case Study of Synergy between Chlorpyrifos and CHD8 Knockout in Human BrainSpheres.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio Modafferi,Xiali Zhong,Andre Kleensang,Yohei Murata,Francesca Fagiani,David Pamies,Helena T Hogberg,Vittorio Calabrese,Herbert Lachman,Thomas Hartung,Lena Smirnova

Turkish journal of pharmaceutical sciences 17:506-510 PubMed33177931

2020

Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Sevİm,Ali Taghİzadehghalehjoughİ,Mehtap Kara
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