Acetyltransferase Activity Assay Kit (Fluorometric)
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(5 Publications)
Acetyltransferase Activity Assay Kit ab204536 is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity.
View Alternative Names
ACTL, ACAT2, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase
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Supplier Data
Functional Studies - Acetyltransferase Activity Assay Kit (Fluorometric) (AB204536)
Example Kinetic Assay Results.
100nM CAT was tested with 100μM Chloramphenicol substrate in the kinetic assay format. Mean relative fluorescence was plotted against the stop time interval to generate this graph. Obtained using Abcam's Acetyltransferase Activity Assay Kit (Fluorometric) (ab204536).
- FuncS
Supplier Data
Functional Studies - Acetyltransferase Activity Assay Kit (Fluorometric) (AB204536)
Chloramphenicol acetyltransferase (CAT) titration in acetyltransferase assay.
Chloramphenicol acetyltransferase (CAT) was titered in the assay using 100μM of the Substrate chloramphenicol. Serial dilutions of the enzyme were prepared in Transferase Assay Buffer. Mean relative fluorescence was plotted against CAT concentration to generate the following graph.
This is for illustration purposes only.
The investigator must titrate their enzyme / substrate system in the assay.
Based on this titration data, the acetyltransferase concentration of 100nM produces a maximum signal within the detection range of the plate reader, with a signal to noise ratio sufficient for easy detection of altered enzyme activity.
- FuncS
Supplier Data
Functional Studies - Acetyltransferase Activity Assay Kit (Fluorometric) (AB204536)
Typical inhibition curve obtained using Abcam's Acetyltransferase Activity Assay Kit (Fluorometric) (ab204536)
Reactivity data
Product details
Acetyltransferase Activity Assay Kit (Fluorometric) (ab204536) is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
This assay is a complete kit for the screening of candidate compounds that can alter normal acetyltransferase activity. For use with purified in vitro samples.
Acetylation is an important covalent molecular modification. Originally identified as the method by which certain bacteria were able to deactivate anti-microbial compounds, acetylation is now also known as an important partitioning and signaling modification.
Acetyltransferases are enzymes that covalently transfer an acetyl group from a donor molecule (Acetyl CoA) to an acceptor. Acetyl CoA serves as a universal donor while the acceptor varies with the acetyltransferase. Acceptors include histones, kinases, transcription factors, receptors, neurotransmitter precursors like choline and serotonin, and anti-microbial agents like chloramphenicol and fluoroquinones. Acetylation can signal an increase or decrease in activity based on the context of the message. Frequently located at critical junctions in metabolic pathways, Acetyltransferases and their regulation have become attractive therapeutic targets to treat everything from insomnia to cancer.
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Supplementary information
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Biological function summary
This enzyme facilitates the synthesis of cholesterol and ketone bodies essential processes for cellular energy balance. ACAT2 works as an independent enzyme rather than as a component of a multi-enzyme complex unlike some other acetyltransferases. By managing acetyl-CoA levels it supports energy homeostasis and lipogenesis providing substrates for downstream processes that are critical for sustaining cellular function and response to energy demands.
Pathways
The acetyl-transfer reactions catalyzed by ACAT2 are integral to the cholesterol biosynthesis and ketogenesis pathways. These pathways are important for energy balance and lipid modification. Within these pathways ACAT2 acts in close relation with HMG-CoA synthase an important player in cholesterol biosynthesis. The enzyme’s actions provide acetoacetyl-CoA which is a substrate for the production of HMG-CoA highlighting its role in maintaining metabolic flux.
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Publications (5)
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Signal transduction and targeted therapy 6:325 PubMed34465723
2021
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Antimicrobial agents and chemotherapy 65: PubMed33106268
2020
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BioMed research international 2019:8714363 PubMed31828139
2019
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Cell chemical biology 24:133-140 PubMed28132892
2017
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ACS chemical biology 12:635-642 PubMed28094496
2017
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