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AB273291

Acidic Mammalian Chitinase Activity Kit (Fluorometric)

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Acidic Mammalian Chitinase Activity Kit (Fluorometric) (ab273291) utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity.

View Alternative Names

Acidic mammalian chitinase, AMCase, Lung-specific protein TSA1902, CHIA

3 Images
Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)
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Supplier Data

Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)

Measurement of AMCase activity in different tissues.

Measurement of AMCase activity in Rat Liver (20 μg protein) and Mouse Lung (2.5 μg protein). All assays were performed following kit protocol.

Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)
  • FuncS

Supplier Data

Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

4-MU Standard Curve. Results from multiple experiments.

Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)
  • FuncS

Supplier Data

Functional Studies - Acidic Mammalian Chitinase Activity Kit (Fluorometric) (AB273291)

AMCase Activity graph.

Measurement of purified AMCase (2 ng) and CHIT1 (2 ng) activities. The kit can effectively discriminate AMCase activity from CHIT1 activity using our proprietary Lysis Buffer.

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Tissue, Suspension cells, Adherent cells, Cell Lysate

Assay type

Enzyme activity (quantitative)

Sensitivity

= 0.5 mU/mL

Assay Platform

Microplate

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Product details

Acidic Mammalian Chitinase Activity Kit (Fluorometric) (ab273291) utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity. This kit provides a simple, specific, ultra-sensitive assay that can detect as low as 0.5 mU/ml of AMCase activity in a variety of biological samples.

This product is manufactured by BioVision, an Abcam company and was previously called K513 Acidic Mammalian Chitinase Activity Kit (Fluorometric). K513-100 is the same size as the 100 test size of ab273291.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273291-100Test", "assetComponentDetails": [ { "size":"1 x 18 mL", "name":"AMCase Lysis Buffer", "number":"AB273291-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"AMCase Assay Buffer", "number":"AB273291-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Acidic Mammalian Chitinase (Lyophilized)", "number":"AB273291-CMP01", "productcode":"" }, { "size":"1 x 35 µL", "name":"4-Methylumbelliferone Standard (5 mM)", "number":"AB273291-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"AMCase Substrate", "number":"AB273291-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
See full target information CHIA
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