Aconitase Assay Kit ab83459 is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.
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- Antioxidants (Basel, Switzerland) 12:2023Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert T Mankowski,Stephanie E Wohlgemuth,Guilherme Bresciani,A Daniel Martin,George Arnaoutakis,Tomas Martin,Eric Jeng,Leonardo Ferreira,Tiago Machuca,Mindaugas Rackauskas,Ashley J Smuder,Thomas Beaver,Christiaan Leeuwenburgh,Barbara K SmithPubMed 37237876
- Environmental health perspectives 129:870052021Chronic Manganese Exposure and the Enteric Nervous System: An and Mouse Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShivani Ghaisas,Dilshan S Harischandra,Bharathi Palanisamy,Alexandra Proctor,Huajun Jin,Somak Dutta,Souvarish Sarkar,Monica Langley,Gary Zenitsky,Vellareddy Anantharam,Arthi Kanthasamy,Gregory J Phillips,Anumantha KanthasamyPubMed 34410835
- Oncology letters 22:6572021Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengping Yao,Xiaohong Cui,Ying Zhang,Zhuchun Bei,Hongquan Wang,Dongxu Zhao,Hong Wang,Yongfei YangPubMed 34386079
- Orphanet journal of rare diseases 16:2012021Down regulation of the expression of mitochondrial phosphopantetheinyl-proteins in pantothenate kinase-associated neurodegeneration: pathophysiological consequences and therapeutic perspectives.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMónica Álvarez-Córdoba,Marta Talaverón-Rey,Irene Villalón-García,Suleva Povea-Cabello,Juan M Suárez-Rivero,Alejandra Suárez-Carrillo,Manuel Munuera-Cabeza,Joaquín J Salas,José A Sánchez-AlcázarPubMed 33952316
- Cell metabolism 33:957-970.e62021Excessive exercise training causes mitochondrial functional impairment and decreases glucose tolerance in healthy volunteers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikael Flockhart,Lina C Nilsson,Senna Tais,Björn Ekblom,William Apró,Filip J LarsenPubMed 33740420
- Journal of clinical medicine 9:2020Mitochondrial Energetics and Ca2-Activated ATPase in Obstructive Hypertrophic Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Lombardi et. al.PubMed 32527005
- Molecular cell 77:645-655.e72020Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoss A Weber et. al.PubMed 31983508
- PloS one 13:e02021512018Pseudomonas aeruginosa nfuA: Gene regulation and its physiological roles in sustaining growth under stress and anaerobic conditions and maintaining bacterial virulence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdisak Romsang et. al.PubMed 30092083
- Journal of neurochemistry 142:429-4432017Sonic hedgehog signalling mediates astrocyte crosstalk with neurons to confer neuroprotection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher I Ugbode et. al.PubMed 28485896
- Antioxidants & redox signaling 27:1048-10662017Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Langley et. al.PubMed 28375739
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
40m
Associated Products
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Target data
Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Targets
ACO1
Alternative names
Aconitase, Citrate hydro-lyase, ACO2
What's included?
Recommended products
Aconitase Assay Kit ab83459 is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.
Alternative names
Aconitase, Citrate hydro-lyase, ACO2
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Assay time
40m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Aconitase Assay Kit ab83459 is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples.
In the aconitase assay protocol, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a max absorbance at 450nm.
Aconitase assay protocol summary:
- activate aconitase in samples by adding cysteine HCl and (NH4)2Fe(SO4)2
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K716 Aconitase Activity Colorimetric Assay Kit. K716-100 is the same size as the 100 test size of ab83459.
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aconitase also known as aconitate hydratase is an enzyme with a mass of approximately 82 kDa. It catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid (TCA) cycle. Aconitase is expressed in the mitochondria where it functions as mitochondrial aconitase and in the cytoplasm in a slightly different form. The enzyme contains an iron-sulfur cluster that is important for its activity. Scientists often use aconitase kits to measure its activity in different samples.
- Biological function summary
Mitochondrial aconitase plays an important role in energy production by facilitating the TCA cycle which is essential for ATP generation. The enzyme can also serve as an iron regulatory protein in the cytoplasm contributing to the regulation of iron metabolism. Aconitase exists as part of a larger complex with its iron-sulfur cluster playing a critical role in both its enzymatic and regulatory functions. By shifting between its enzymatic and regulatory roles aconitase helps in maintaining homeostasis within cells.
- Pathways
Aconitase operates in the TCA cycle an important metabolic pathway for energy production. It participates in the metabolism of acetyl-CoA converting it into isocitrate through interactions with citrate. This key pathway integrates with glycolysis and aids in the oxidative phosphorylation process. Aconitase's function is related to proteins such as isocitrate dehydrogenase which further processes isocitrate in the TCA cycle.
- Associated diseases and disorders
Mutations and defects in aconitase can contribute to neurodegenerative diseases and iron-sulfur cluster deficiencies. A connection exists between aconitase activity and disorders like Friedreich’s ataxia a condition featuring impaired iron-sulfur cluster biosynthesis. Ferritin a protein associated with iron storage links with aconitase through pathways involved in iron regulation suggesting potential therapeutic targets in disorders of iron metabolism.
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4 product images
Cummins, Nadia et al., BMC neuroscience?vol. 14 98., Fig 6, doi:10.1186/1471-2202-14-98Functional Studies - Aconitase Assay Kit (ab83459)
Mitochondrial autophagic profiles in ON vulnerable to secondary degeneration +/- R/NIR-IT. Accumulations of mitochondrial autophagic profiles (red arrows) were seen especially at day 1 after PT (A, B). Occasionally, larger features reminiscent of autophagosomes were observed (A, black arrow) and parts of the nerve appeared highly abnormal (*, B).
Functional Studies - Aconitase Assay Kit (ab83459)
Aconitase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
Protein concentration for samples varied from 1.5 mg/mL to 10 mg/mL. Samples were diluted 1-27 fold.
Functional Studies - Aconitase Assay Kit (ab83459)
30 minute Aconitase sample test using ab83459.
Functional Studies - Aconitase Assay Kit (ab83459)
Isocitrate standard curve using ab83459.
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