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AB273315

Acyl-CoA Synthetase Assay Kit (Fluorometric)

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(1 Publication)

Acyl-CoA Synthetase (ACS) Assay Kit (Fluorometric) (ab273315) provides a rapid, sensitive and straightforward way to measure ACS activity in various samples.
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Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)
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Supplier Data

Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)
  • FuncS

Supplier Data

Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)

Reaction kinetics of ACS Positive Control.

Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)
  • FuncS

Supplier Data

Functional Studies - Acyl-CoA Synthetase Assay Kit (Fluorometric) (AB273315)

ACS activity in Rat liver lysate. Rat liver was prepared according to the described protocol. For activity determination, experiments were run in duplicates. 3.3-13.3 μg protein was loaded per well.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Purified protein, Suspension cells, Tissue Extracts, Tissue sections, Adherent cells

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Acyl-CoA Synthetase (ACS) Assay Kit (Fluorometric) (ab273315) provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/μl sample.

ab273315 does not detect acyl-CoA synthetase short chain family members/acetyl-CoA synthetases.

This product is manufactured by BioVision, an Abcam company and was previously called K184 Acyl-CoA Synthetase Fluorometric Assay Kit. K184-100 is the same size as the 100 test size of ab273315.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273315-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB273315-CMP06", "productcode":"AB309437" }, { "size":"1 x 100 µL", "name":"H2O2 Standard", "number":"AB273315-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB273315-CMP01", "productcode":"AB323940" }, { "size":"1 x 1 Vial", "name":"Acyl CoA Enzyme Mix", "number":"AB273315-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"ACS Substrate", "number":"AB273315-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ACS Positive Control", "number":"AB273315-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ACS Developer", "number":"AB273315-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ACS Converter", "number":"AB273315-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acyl-CoA synthetase (ACS) also known as acyl-coenzyme A synthetase is an enzyme that catalyzes the conversion of free fatty acids into acyl-CoA which is then used in various metabolic processes. It functions by activating fatty acids through a thioester bond with CoA a necessary first step in several metabolic pathways. The enzyme generally has a molecular mass around 72 kDa though this varies slightly among different isoforms. ACS enzymes are expressed in diverse tissues including the liver adipose tissue and muscle reflecting their essential role in lipid metabolism.
Biological function summary

These enzymes are critical for converting long-chain fatty acids into acyl-CoA which can then enter beta-oxidation or be used in lipid biosynthesis. ACS operates as a monomer but might be associated with multi-enzyme complexes for efficient substrate channeling. This action ensures effective regulation and partitioning of fatty acids between different cellular pathways. These enzymes play an important part in cellular energy homeostasis influencing lipid and carbohydrate metabolism.

Pathways

These enzymes play roles in fatty acid metabolism and energy production. Acyl-CoA synthetases actively participate in the beta-oxidation pathway of fatty acids a vital process for generating ATP from lipid stores. They also intersect with the synthesis of membrane lipids facilitated by proteins like acetyl-CoA carboxylase and acetyl-CoA synthetase. Their proper function ensures the balance between energy storage and consumption critical for metabolic health.

Dysregulation of ACS activity is associated with metabolic syndromes such as obesity and non-alcoholic fatty liver disease (NAFLD). Their dysfunction can disrupt normal lipid metabolism leading to abnormal fat accumulation and insulin resistance. Connections with other metabolic enzymes like acetyl-CoA carboxylase have also been observed suggesting that alterations in ACS function might influence broader metabolic networks. Understanding the role of these enzymes in such disorders could help develop targeted therapies for metabolic diseases.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1338-1353.e8 PubMed38503284

2024

Anti-apoptotic MCL-1 promotes long-chain fatty acid oxidation through interaction with ACSL1.

Applications

Unspecified application

Species

Unspecified reactive species

Tristen Wright,Meghan E Turnis,Christy R Grace,Xiao Li,Lauren A Brakefield,Yong-Dong Wang,Haiyan Xu,Ewa Kaminska,Leslie K Climer,Tresor O Mukiza,Chi-Lun Chang,Tudor Moldoveanu,Joseph T Opferman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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