Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)

Product details

Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA convertor and developer to generate uric acid that can be easily quantified at OD293 nm.

The kit measures total activity of Adenosine Deaminase with limit of quantification of 1 mU recombinant Adenosine Deaminase.

OTher Notes
This product is manufactured by BioVision, an Abcam company and was previously called K321 Adenosine Deaminase Activity Assay Kit (Colorimetric). K321-100 is the same size as the 100 test size of ab211093.

Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that catalyses the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and has been found associated with DPP4/CD26 in the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.

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