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AB204695

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)

4

(1 Review)

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(9 Publications)

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product that can be easily quantified at Ex/Em = 535/587 nm.

View Alternative Names

Adenosine deaminase, Adenosine aminohydrolase, Ada

3 Images
Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)
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Lab

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

Adenosine Deaminase Activity Assay pathway

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)
  • FuncS

Lab

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

Typical Inosine Standard Curve

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)
  • FuncS

Supplier Data

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 μg).

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Tissue Lysate, Cell culture media, Cell Lysate

Assay type

Enzyme activity

Sensitivity

= 5 µU

Range

10 - 100 µU

Assay Platform

Microplate reader

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Reactivity data

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Product details

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (ab204695) is an assay where inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product that can be easily quantified at Ex/Em = 535/587 nm. The kit measures total activity of Adenosine Deaminase with limit of quantification of 10 μU recombinant Adenosine Deaminase. The range of the assay is from 10-100 μU.

Other notes
This was previously called K328 Biovision Adenosine Deaminase Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that catalyses the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and has been found associated with DPP4/CD26 in the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADA) sometimes known as deaminase is an enzyme that plays a critical mechanical role in cellular metabolism. This enzyme catalyzes the deamination of adenosine converting it into inosine. ADA is a 41 kDa protein primarily expressed in most tissues including the thymus and spleen. Various ADA assays and ADA protocols exist to study its functionality and quantification often using ADA ELISA kits to measure ADA activity in biological samples. In cell lines such as HeLa ADA expression is also present and significant for studying its regulation and function.
Biological function summary

Adenosine deaminase activity influences purine metabolism playing an important role in the breakdown of adenosine. It is not part of a complex but interacts closely with other components of the purine salvage pathway to maintain nucleotide balance. This enzyme is important for the proper function of immune cells as it prevents toxic accumulations of adenosine which can be detrimental to lymphocyte proliferation and function. ADA's activity ensures the proper management of nucleotides within cells sustaining cellular health and functionality.

Pathways

Adenosine deaminase plays significant roles in the immune and purine catabolism pathways. Within these pathways ADA is closely related to proteins such as purine nucleoside phosphorylase (PNP) which together orchestrate the steps necessary for adenine and guanine nucleotide turnover. These proteins facilitate the conversion processes needed for cellular nucleic acid synthesis and energy regulation. By managing adenosine levels ADA's function ensures the smooth operation of cellular signaling and metabolic balance.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) and can also be associated with pulmonary disorders like asthma. A lack of ADA activity leads to the accumulation of toxic metabolites impairing immune function and contributing to the pathogenesis of SCID. In some patients with pulmonary disorders altered ADA activity impacts the inflammatory response. ADA's mechanistic interactions with other proteins such as PNP highlight its importance in maintaining immune competence and metabolic homeostasis emphasizing the enzyme's role in these clinical conditions.
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Product protocols

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Target data

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed : 19900420). Plays an important role in purine metabolism and in adenosine homeostasis (By similarity). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (By similarity). Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (By similarity) (PubMed : 19900420). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (By similarity).
See full target information Ada

Additional targets

ADA,Ada
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107425 PubMed38823639

2024

A crucial role of adenosine deaminase in regulating gluconeogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Ding,Wenhao Ge,Xiaogang Xu,Xiaodong Xu,Qi Sun,Xi Xu,Jianfa Zhang

Nature communications 14:1367 PubMed36914661

2023

The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle.

Applications

Unspecified application

Species

Unspecified reactive species

Suba Rajendren,Xiang Ye,William Dunker,Antiana Richardson,John Karijolich

Frontiers in endocrinology 13:1007801 PubMed36407311

2022

Resveratrol enhances A and hinders A adenosine receptors signaling in both HeLa and SH-SY5Y cells: Potential mechanism of its antitumoral action.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Muñoz-López,Alejandro Sánchez-Melgar,Mairena Martín,José Luis Albasanz

Frontiers in neuroscience 15:702817 PubMed34539333

2021

Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Sánchez-Melgar,Sonia Muñoz-López,José Luis Albasanz,Mairena Martín

Cell host & microbe 29:1351-1365.e11 PubMed34403684

2021

Gut microbiome ADP-ribosyltransferases are widespread phage-encoded fitness factors.

Applications

Unspecified application

Species

Unspecified reactive species

Eric M Brown,Hugo Arellano-Santoyo,Emily R Temple,Zachary A Costliow,Matthieu Pichaud,A Brantley Hall,Kai Liu,Michael A Durney,Xiebin Gu,Damian R Plichta,Clary A Clish,Jeffrey A Porter,Hera Vlamakis,Ramnik J Xavier

International journal of molecular sciences 21: PubMed33023260

2020

Adenosine Metabolism in the Cerebral Cortex from Several Mice Models during Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Sánchez-Melgar,José Luis Albasanz,Mercè Pallàs,Mairena Martín

Cell reports 31:107564 PubMed32348766

2020

ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huirong Zhang,Guoxin Ni,Blossom Damania

Molecular neurobiology 56:2881-2895 PubMed30069829

2018

Resveratrol Modulates and Reverses the Age-Related Effect on Adenosine-Mediated Signalling in SAMP8 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

A Sánchez-Melgar,J L Albasanz,V Palomera-Ávalos,M Pallàs,M Martín

Stem cell research & therapy 9:184 PubMed29973267

2018

Human mesenchymal stromal cells inhibit platelet activation and aggregation involving CD73-converted adenosine.

Applications

Unspecified application

Species

Unspecified reactive species

P Netsch,S Elvers-Hornung,S Uhlig,H Klüter,V Huck,F Kirschhöfer,G Brenner-Weiß,K Janetzko,H Solz,P Wuchter,P Bugert,K Bieback
View all publications
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