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AB323608

Adenylate Cyclase Activity Assay

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The Adenylate Cyclase Activity Assay Kit (ab323608) is a simple, sensitive kit that measures the conversion of ATP to cAMP and pyrophosphate using a propriety enzymatic detection mix and a chromogenic probe to generate a stable chromophore that can be quantified by absorbance.
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Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
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Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Adenylate Cyclase Activity Assay Kit reaction pathway

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
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Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Example standard curve (0-10 nmol) used to determine the activity of adenylate cyclase. The change in absorbance over a time range for a sample is used to determine the nmol of PPi generated due to adenylate cyclase activity in a defined time frame. ΔAbsorbance must be within the range of the standard curve.

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
  • FuncS

Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Example midrange positive control data. The linear range for this positive control occurs between 5 and 18 minutes. Prior to five minutes, the reaction is coming to temperature and is experiencing a lag as the enzyme ramps up, after 18 minutes the substrate is becoming depleted and activity is slowing. Choosing a time interval within the linear range for each sample is critical to accurately quantify adenylate cyclase activity.

Biochemical assay - Adenylate Cyclase Activity Assay (AB323608)
  • Biochemical assay

Supplier Data

Biochemical assay - Adenylate Cyclase Activity Assay (AB323608)

Adenylate Cyclase activity in cell and tissue lysates. Samples were lysed according to the sample preparation protocol and 4 µl of each sample was tested. Specific activity was calculated from sample concentration determined using a Bradford assay.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

< 0.02 mU

Range

0.02 - 1 mU

Assay time

1h 15m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biochemical assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Adenylate Cyclase Activity assay kit is a simple, sensitive kit that measures the conversion of ATP to cAMP and pyrophosphate using a propriety enzymatic detection mix and a chromogenic probe to generate a stable chromophore that can be quantified by absorbance.

In the first step of this enzymatic assay, Adenylyl Cyclase (Adenylate Cyclase) converts ATP into cAMP, releasing inorganic pyrophosphate (PPi). The PPi released is detected via a series of enzymatic reactions which utilize a propriety enzyme mix and a chromogenic probe, generating a stable chromophore that can be quantified by absorbance at 570 nm.

First functional test on catalogue, others are ELISA based and either only measure cAMP levels or AC protein concentration.

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What's included?

{ "values": { "1x100Tests": { "sellingSize": "1 x 100 Tests", "publicAssetCode":"ab323608-1x100Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Pyrophosphate Standard", "number":"AB323608-CMP08", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB323608-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB323608-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Enzyme Mix X", "number":"AB323608-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"AC Substrate Mix", "number":"AB323608-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 15", "number":"AB323608-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"AC Positive Control", "number":"AB323608-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"ATP IV", "number":"AB323608-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Adenylate Cyclase
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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