Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Frontiers in immunology 14:12307722023Integrated transcriptomic and metabolomic analysis reveals the metabolic programming of GM-CSF- and M-CSF- differentiated mouse macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianyue Zhang,Qiaoling Song,Shan Liu,Yuting Xu,Danling Gao,Peizhe Lu,Yuantao Liu,Guanghui Zhao,Lihong Wu,Chenyang Zhao,Jinbo YangPubMed 37818352
- Science advances 7:eabj34002021Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Seok Lee,Sung-Bae Lee,Dong-Woon Kim,Nara Shin,Seon-Ju Jeong,Chae-Ha Yang,Chang-Gue SonPubMed 34739315
- PloS one 16:e02531682021Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParag Goyal,Harish Venkatraman Pai,Phanichand Kodali,Bhavesh Vats,Navratna Vajpai,Shankara Annegowda,Krishnappa Mane,Shamini Mohan,Shruti Saxena,Anil Bangalore Veerabhadraia,Milee Palande,Preethy Sasankan Nair,Digvijay Chandrashekar More,Umamaheshwara Rao Karudumpa,Kunala Jyothirmai,Adroha Bhattacharya,Frida Almeida,Santosh Gulab Khyade,Shankara Gouda,Daniel J Ranayhossaini,Praveen Reddy Moole,Jeffrey P Smith,Abhijit Barve,Ramakrishnan Melarkode,Rajesh UllanatPubMed 34133466
- PLoS biology 18:e30007392020Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabeth Stelling et. al.PubMed 33370269
- Science advances 6:eaay67212020Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoun Jae Jung et. al.PubMed 32232152
- Laboratory investigation; a journal of technical methods and pathology 100:553-5692019Maternal diet intervention before pregnancy primes offspring lipid metabolism in liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhou,Hui Peng,Huiting Xu,Jiangyuan Li,Mikhail Golovko,Henghui Cheng,Ernest C Lynch,Lin Liu,Naomi McCauley,Lindsey Kennedy,Gianfranco Alpini,Ke K Zhang,Linglin XiePubMed 31748681
- Molecular medicine reports 20:4119-41242019Hydrogen sulfide protects against high glucose‑induced lipid metabolic disturbances in 3T3‑L1 adipocytes via the AMPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Pan,Jue Wang,Min Xu,Shihong Chen,Xiaobo Li,Aili Sun,Nengjun Lou,Yihong NiPubMed 31545435
- The Journal of nutritional biochemistry 62:210-2202018A long-term maternal diet intervention is necessary to avoid the obesogenic effect of maternal high-fat diet in the offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiting Xu,Qiang Fu,Yi Zhou,Chengbin Xue,Patrick Olson,Ernest C Lynch,Ke K Zhang,Chaodong Wu,Peter Murano,Lanjing Zhang,Linglin XiePubMed 30316166
- Lipids 53:581-5882018Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBreetha Ramaiyan,Ramaprasad Ravichandra TalahalliPubMed 30203512
- Lipids in health and disease 16:1812017Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Range
- 0.2 - 10 nM
- Assay time
- 40m
- Sensitivity
- > 0.2 nM
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Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Range
- 0.2 - 10 nM
- Assay time
- 40m
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.2 nM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K610 Adipogenesis Colorimetric/Fluorometric Assay Kit. K610-100 is the same size as the 100 test size of ab102513.
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adipogenesis also known as fat cell differentiation refers to the process of creating mature adipocytes from precursor cells such as mesenchymal stem cells. The process involves different stages marked by the expression of several key transcription factors like C/EBPα and PPARγ. These factors regulate the conversion of preadipocytes into fully functional adipocytes. Primary sites for adipogenesis include white and brown adipose tissues and this process can be studied using various detection methods including fluorometric detection and adipored assays.
- Biological function summary
The formation of adipocytes involves the activation of specific gene networks that govern lipid accumulation and insulin sensitivity. Adipogenesis plays a significant role in energy storage and homeostasis by having the function of sequestering lipids in the form of triglycerides. It involves complex signaling pathways where multiple proteins interact and coordinate to facilitate lipid droplet formation and adipocyte maturation.
- Pathways
Adipocyte development is tightly integrated into the insulin signaling and lipid metabolism pathways. Within these pathways adipogenesis interacts with proteins like AKT and mTOR which are important for promoting lipid accumulation and maintaining energy balance. The pathways form a coordinated system that ensures efficient energy storage and mobilization depending on metabolic demands.
- Associated diseases and disorders
Improper regulation of adipogenesis is linked with metabolic diseases such as obesity and type 2 diabetes. Dysregulated adipocyte formation can contribute to ectopic lipid accumulation and insulin resistance. The process of adipogenesis influences pathological states through proteins such as TNF-α which is elevated in these disorders and negatively impacts adipocyte function. Proper understanding and manipulation of this process offer potential therapeutic avenues for controlling obesity-related complications.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Example of a standard curve obtained with ab102513.
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Example of a standard curve obtained with ab102513.
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Glycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Glycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample.
Ms brain value retrieved from 1:9 dilution of original 12.4mg/mL sample.
Ms muscle value retrieved from 1:9 dilution of original 7.5mg/mL sample.
Ms heart value retrieved from 1:27 dilution of original 16.4mg/mL sample.
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Glycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells
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