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AB102513

Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)

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(17 Publications)

Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
6 Images
Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
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Lab

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Glycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
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Lab

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Glycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample.

Ms brain value retrieved from 1 : 9 dilution of original 12.4mg/mL sample.

Ms muscle value retrieved from 1 : 9 dilution of original 7.5mg/mL sample.

Ms heart value retrieved from 1 : 27 dilution of original 16.4mg/mL sample.

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
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Supplier Data

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Example of a standard curve obtained with ab102513.

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
  • FuncS

Supplier Data

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Example of a standard curve obtained with ab102513.

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
  • FuncS

Lab

Functional Studies - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Glycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

Schematic Diagram - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (AB102513)

Representative image of Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) ab102513

Components shown from left to right :

- OxiRed Probe

- Lipase I

- Triglyceride Standard

- Enzyme Mix VI

- Assay Buffer 5

- Adipogenesis Lipid Extraction Solution

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Sensitivity

> 0.2 nM

Range

0.2 - 10 nM

Assay time

40m

Assay Platform

Microplate reader

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Product details

Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K610 Adipogenesis Colorimetric/Fluorometric Assay Kit. K610-100 is the same size as the 100 test size of ab102513.

Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adipogenesis also known as fat cell differentiation refers to the process of creating mature adipocytes from precursor cells such as mesenchymal stem cells. The process involves different stages marked by the expression of several key transcription factors like C/EBPα and PPARγ. These factors regulate the conversion of preadipocytes into fully functional adipocytes. Primary sites for adipogenesis include white and brown adipose tissues and this process can be studied using various detection methods including fluorometric detection and adipored assays.
Biological function summary

The formation of adipocytes involves the activation of specific gene networks that govern lipid accumulation and insulin sensitivity. Adipogenesis plays a significant role in energy storage and homeostasis by having the function of sequestering lipids in the form of triglycerides. It involves complex signaling pathways where multiple proteins interact and coordinate to facilitate lipid droplet formation and adipocyte maturation.

Pathways

Adipocyte development is tightly integrated into the insulin signaling and lipid metabolism pathways. Within these pathways adipogenesis interacts with proteins like AKT and mTOR which are important for promoting lipid accumulation and maintaining energy balance. The pathways form a coordinated system that ensures efficient energy storage and mobilization depending on metabolic demands.

Improper regulation of adipogenesis is linked with metabolic diseases such as obesity and type 2 diabetes. Dysregulated adipocyte formation can contribute to ectopic lipid accumulation and insulin resistance. The process of adipogenesis influences pathological states through proteins such as TNF-α which is elevated in these disorders and negatively impacts adipocyte function. Proper understanding and manipulation of this process offer potential therapeutic avenues for controlling obesity-related complications.
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Product protocols

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Target data

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1174 PubMed39294274

2024

CTBP1 links metabolic syndrome to polycystic ovary syndrome through interruption of aromatase and SREBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Kong,Guang Yang,Xu Feng,Zhaodong Ji,Xiaoling Wang,Yang Shao,Jiao Meng,Guidong Yao,Chunxia Ren,Gong Yang

Frontiers in immunology 14:1230772 PubMed37818352

2023

Integrated transcriptomic and metabolomic analysis reveals the metabolic programming of GM-CSF- and M-CSF- differentiated mouse macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyue Zhang,Qiaoling Song,Shan Liu,Yuting Xu,Danling Gao,Peizhe Lu,Yuantao Liu,Guanghui Zhao,Lihong Wu,Chenyang Zhao,Jinbo Yang

Science advances 7:eabj3400 PubMed34739315

2021

Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Seok Lee,Sung-Bae Lee,Dong-Woon Kim,Nara Shin,Seon-Ju Jeong,Chae-Ha Yang,Chang-Gue Son

PloS one 16:e0253168 PubMed34133466

2021

Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine.

Applications

Unspecified application

Species

Unspecified reactive species

Parag Goyal,Harish Venkatraman Pai,Phanichand Kodali,Bhavesh Vats,Navratna Vajpai,Shankara Annegowda,Krishnappa Mane,Shamini Mohan,Shruti Saxena,Anil Bangalore Veerabhadraia,Milee Palande,Preethy Sasankan Nair,Digvijay Chandrashekar More,Umamaheshwara Rao Karudumpa,Kunala Jyothirmai,Adroha Bhattacharya,Frida Almeida,Santosh Gulab Khyade,Shankara Gouda,Daniel J Ranayhossaini,Praveen Reddy Moole,Jeffrey P Smith,Abhijit Barve,Ramakrishnan Melarkode,Rajesh Ullanat

PLoS biology 18:e3000739 PubMed33370269

2020

Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Stelling,Melanie Ricke-Hoch,Sergej Erschow,Steve Hoffmann,Anke Katharina Bergmann,Maren Heimerl,Stefan Pietzsch,Karin Battmer,Alexandra Haase,Britta Stapel,Michaela Scherr,Jean-Luc Balligand,Ofer Binah,Denise Hilfiker-Kleiner

Science advances 6:eaay6721 PubMed32232152

2020

Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Youn Jae Jung,Hark Kyun Kim,Yoonsuk Cho,Ji Suk Choi,Chang Hee Woo,Kyoung Soo Lee,Jae Hoon Sul,Chan Mi Lee,Jihoon Han,Jae Hyung Park,Dong-Gyu Jo,Yong Woo Cho

Laboratory investigation; a journal of technical methods and pathology 100:553-569 PubMed31748681

2019

Maternal diet intervention before pregnancy primes offspring lipid metabolism in liver.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhou,Hui Peng,Huiting Xu,Jiangyuan Li,Mikhail Golovko,Henghui Cheng,Ernest C Lynch,Lin Liu,Naomi McCauley,Lindsey Kennedy,Gianfranco Alpini,Ke K Zhang,Linglin Xie

Molecular medicine reports 20:4119-4124 PubMed31545435

2019

Hydrogen sulfide protects against high glucose‑induced lipid metabolic disturbances in 3T3‑L1 adipocytes via the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Pan,Jue Wang,Min Xu,Shihong Chen,Xiaobo Li,Aili Sun,Nengjun Lou,Yihong Ni

The Journal of nutritional biochemistry 62:210-220 PubMed30316166

2018

A long-term maternal diet intervention is necessary to avoid the obesogenic effect of maternal high-fat diet in the offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Huiting Xu,Qiang Fu,Yi Zhou,Chengbin Xue,Patrick Olson,Ernest C Lynch,Ke K Zhang,Chaodong Wu,Peter Murano,Lanjing Zhang,Linglin Xie

Lipids 53:581-588 PubMed30203512

2018

Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Breetha Ramaiyan,Ramaprasad Ravichandra Talahalli
View all publications
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