ADP Assay Kit ab83359 provides a convenient colorimetric and fluorometric method to measure ADP level.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Frontiers in endocrinology 14:12377962023Adiponectin and resistin modulate the progression of Alzheimer´s disease in a metabolic syndrome model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Cisternas,Camila Gherardelli,Joel Gutierrez,Paulina Salazar,Carolina Mendez-Orellana,G William Wong,Nibaldo C InestrosaPubMed 37732123
- Experimental cell research 424:1135072023ACTA1 H40Y mutant iPSC-derived skeletal myocytes display mitochondrial defects in an in vitro model of nemaline myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie Gartz,Margaret Haberman,Jessica Sutton,Rebecca A Slick,Shawn M Luttrell,David L Mack,Michael W LawlorPubMed 36796746
- International journal of molecular sciences 23:2022MgADP Promotes Myosin Head Movement toward Actin at Low [Ca] to Increase Force Production and Ca-Sensitivity of Contraction in Permeabilized Porcine Myocardial Strips.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter O Awinda,Weikang Ma,Kyrah L Turner,Jing Zhao,Henry Gong,Mindy S Thompson,Kenneth S Campbell,Thomas C Irving,Bertrand C W TannerPubMed 36499408
- Genetics and molecular biology 45:e202103702022Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Liu,Yongrui Du,Hui Li,Xuexia LinPubMed 36121916
- International journal of molecular sciences 23:2022Lithium Enhances Hippocampal Glucose Metabolism in an In Vitro Mice Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamila Gherardelli,Pedro Cisternas,Nibaldo C InestrosaPubMed 35955868
- Nature communications 13:39562022Lipolysis regulates major transcriptional programs in brown adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLasse K Markussen,Elizabeth A Rondini,Olivia Sveidahl Johansen,Jesper G S Madsen,Elahu G Sustarsic,Ann-Britt Marcher,Jacob B Hansen,Zachary Gerhart-Hines,James G Granneman,Susanne MandrupPubMed 35803907
- Cells 11:2022Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamalnath S Rajagopalan,Logan M Glasstetter,Xiang-Yang Zhu,Roman Thaler,Hui Tang,Kyra L Jordan,Ishran M Saadiq,Sandra M Herrmann,Alejandro R Chade,Maria V Irazabal,Lilach O Lerman,Alfonso EirinPubMed 35681498
- Developmental cell 56:811-825.e62021Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanchun Yang,Dejiang Pang,Mina Chen,Chongyangzi Du,Lanlan Jia,Luoling Wang,Yunling He,Wanxiang Jiang,Liping Luo,Zongyan Yu,Mengqian Mao,Qiuyun Yuan,Ping Tang,Xiaoqiang Xia,Yiyuan Cui,Bo Jing,Alexander Platero,Yanhui Liu,Yuquan Wei,Paul F Worley,Bo XiaoPubMed 33725483
- Immunity 54:68-83.e62020Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuís Almeida,Ayesha Dhillon-LaBrooy,Carla N Castro,Nigatu Adossa,Guilhermina M Carriche,Melanie Guderian,Saskia Lippens,Sven Dennerlein,Christina Hesse,Bart N Lambrecht,Luciana Berod,Leif Schauser,Bruce R Blazar,Markus Kalesse,Rolf Müller,Luís F Moita,Tim SparwasserPubMed 33238133
- Journal of neurochemistry 157:1222-12332020Andrographolide restores glucose uptake in rat hippocampal neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamila Gherardelli,Pedro Cisternas,Joel Gutiérrez,Milka Martinez,Nibaldo C InestrosaPubMed 33124061
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
1h
- Sensitivity
> 1 µM
What's included?
Recommended products
ADP Assay Kit ab83359 provides a convenient colorimetric and fluorometric method to measure ADP level.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 1 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level.
In the ADP assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm).
Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers.
The ADP assay can detect as low as 1 μM ADP in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K355 ADP Colorimetric/Fluorometric Assay Kit. K355-100 is the same size as the 100 test size of ab83359.
ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADP known as adenosine diphosphate plays an important mechanical role in cellular energy transfer. It forms when ATP adenosine triphosphate releases a phosphate group. This process is fundamental for various cellular activities. ADP itself has a molecular mass of approximately 427.2 g/mol. It is widely expressed in tissues where high energy turnover occurs like muscle cells and neurons. This ubiquity allows it to participate in numerous metabolic reactions facilitating the availability of energy.
- Biological function summary
Energy transfers drive many cellular processes making ADP a critical component of the cell's energy management system. ADP converts back to ATP through phosphorylation primarily occurring in mitochondria. It works as part of larger enzyme complexes including ATP synthase during oxidative phosphorylation. This cycle of conversion maintains the balance of energy within the cell.
- Pathways
ADP is integral to energy and metabolic pathways particularly glycolysis and the Krebs Cycle. During glycolysis ADP receives phosphates becoming ATP and allowing the cell to harness energy. In the Krebs Cycle ADP interacts with electron transport chain components facilitating ATP production via oxidative phosphorylation. The close relationship with ATP and involvement with enzymes like ATP synthase highlight ADP's importance in energy metabolism.
- Associated diseases and disorders
Altered ADP levels can impact metabolic syndromes and cardiovascular conditions. Disruptions in ADP linked pathways may influence conditions like ischemic heart disease where energy supply is compromised. Proteins like ATP synthase and enzymes involved in oxidative phosphorylation are often connected with these disorders as their activity depends on efficient ADP phosphorylation. Identifying problems within ADP handling can therefore be important in understanding and managing related diseases.
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4 product images
Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).
Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.
Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
Colorimetric and Fluorometric examples of ADP Standard curve.
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