AB83359

ADP Assay Kit (Colorimetric/Fluorometric)

Name: ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) | Abcam
Brand: Abcam Limited
SKU: AB83359
Price: 720 USD
Availability: InStock

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(36 Publications)

ADP Assay Kit ab83359 is a mix-and-read assay with one 30-min incubation step at room-temperature. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

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Lab

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

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Supplier Data

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

Colorimetric and Fluorometric examples of ADP Standard curve.

FuncS

Lab

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).

FuncS

Lab

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Sensitivity

> 1 µM

Assay time

Assay Platform

Microplate reader

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Product details

ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level.

ADP assay principle
In the ADP assay, ADP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm).

ADP assay protocol summary
- add samples and standards to wells
- add reaction mix and incubate for 30-min at room temperature
- analyse on plate reader

Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers.

The ADP assay can detect as low as 1 μM ADP in biological samples.

Other notes
This product was previously called K355 Biovision ADP Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADP known as adenosine diphosphate plays an important mechanical role in cellular energy transfer. It forms when ATP adenosine triphosphate releases a phosphate group. This process is fundamental for various cellular activities. ADP itself has a molecular mass of approximately 427.2 g/mol. It is widely expressed in tissues where high energy turnover occurs like muscle cells and neurons. This ubiquity allows it to participate in numerous metabolic reactions facilitating the availability of energy.

Biological function summary

Energy transfers drive many cellular processes making ADP a critical component of the cell's energy management system. ADP converts back to ATP through phosphorylation primarily occurring in mitochondria. It works as part of larger enzyme complexes including ATP synthase during oxidative phosphorylation. This cycle of conversion maintains the balance of energy within the cell.

Pathways

ADP is integral to energy and metabolic pathways particularly glycolysis and the Krebs Cycle. During glycolysis ADP receives phosphates becoming ATP and allowing the cell to harness energy. In the Krebs Cycle ADP interacts with electron transport chain components facilitating ATP production via oxidative phosphorylation. The close relationship with ATP and involvement with enzymes like ATP synthase highlight ADP's importance in energy metabolism.

Altered ADP levels can impact metabolic syndromes and cardiovascular conditions. Disruptions in ADP linked pathways may influence conditions like ischemic heart disease where energy supply is compromised. Proteins like ATP synthase and enzymes involved in oxidative phosphorylation are often connected with these disorders as their activity depends on efficient ADP phosphorylation. Identifying problems within ADP handling can therefore be important in understanding and managing related diseases.

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Product protocols

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Target data

See full target information Adenosine-5'-diphosphate

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e10741 PubMed40385549

2025

Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor-on-a-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Christian R Moya-Garcia,Meghana Munipalle,Alain Pacis,Nader Sadeghi,Maryam Tabrizian,Nicole Y K Li-Jessen

Nature communications 16:2071 PubMed40021646

2025

ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Jianwei You,Xincheng Yang,Ruishu Jiao,Jie Xu,Yue Zhang,Wen Mi,Lingzhi Zhu,Youqiong Ye,Ruobing Ren,Delin Min,Meilin Tang,Li Chen,Fuming Li,Pingyu Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406026 PubMed39494721

2024

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Ouyang,Yaling Li,Haoming Wang,Xiangyang Liu,Xiaoli Tan,Genyuan Xie,Junfa Zeng,Gaofeng Zeng,Qiong Luo,Hong Zhou,Siming Chen,Kai Hou,Jinren Fang,Xia Zhang,Linlin Zhou,Yukun Li,Anbo Gao

Redox report : communications in free radical research 29:2404794 PubMed39314036

2024

WTAP-mediated mA modification of TRIM22 promotes diabetic nephropathy by inducing mitochondrial dysfunction via ubiquitination of OPA1.

Applications

Unspecified application

Species

Unspecified reactive species

Zeng Zhang,Fengzhu Zhou,Min Lu,Duanchun Zhang,Xinyi Zhang,Siyu Xu,Yanming He

Frontiers in endocrinology 14:1237796 PubMed37732123

2023

Adiponectin and resistin modulate the progression of Alzheimer´s disease in a metabolic syndrome model.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Cisternas,Camila Gherardelli,Joel Gutierrez,Paulina Salazar,Carolina Mendez-Orellana,G William Wong,Nibaldo C Inestrosa

The American journal of pathology 193:1528-1547 PubMed37422147

2023

Aberrations in Energetic Metabolism and Stress-Related Pathways Contribute to Pathophysiology in the Neb Conditional Knockout Mouse Model of Nemaline Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca A Slick,Jennifer A Tinklenberg,Jessica Sutton,Liwen Zhang,Hui Meng,Margaret J Beatka,Mark Vanden Avond,Mariah J Prom,Emily Ott,Federica Montanaro,James Heisner,Rafael Toro,Henk Granzier,Aron M Geurts,David F Stowe,R Blake Hill,Michael W Lawlor

Experimental cell research 424:113507 PubMed36796746

2023

ACTA1 H40Y mutant iPSC-derived skeletal myocytes display mitochondrial defects in an in vitro model of nemaline myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Gartz,Margaret Haberman,Jessica Sutton,Rebecca A Slick,Shawn M Luttrell,David L Mack,Michael W Lawlor

International journal of molecular sciences 23: PubMed36499408

2022

MgADP Promotes Myosin Head Movement toward Actin at Low [Ca] to Increase Force Production and Ca-Sensitivity of Contraction in Permeabilized Porcine Myocardial Strips.

Applications

Unspecified application

Species

Unspecified reactive species

Peter O Awinda,Weikang Ma,Kyrah L Turner,Jing Zhao,Henry Gong,Mindy S Thompson,Kenneth S Campbell,Thomas C Irving,Bertrand C W Tanner

Genetics and molecular biology 45:e20210370 PubMed36121916

2022

Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Liu,Yongrui Du,Hui Li,Xuexia Lin

International journal of molecular sciences 23: PubMed35955868

2022

Lithium Enhances Hippocampal Glucose Metabolism in an In Vitro Mice Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Camila Gherardelli,Pedro Cisternas,Nibaldo C Inestrosa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

ADP Assay Kit (Colorimetric/Fluorometric)

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

Functional Studies - ADP Assay Kit (Colorimetric/Fluorometric) (AB83359)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay time

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

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