Advanced Glycation End Products (AGEs) Assay Kit
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(5 Publications)
- FuncS
Supplier Data
Functional Studies - Advanced Glycation End Products (AGEs) Assay Kit (AB273298)
AGE and BSA standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
AGEs-Positive (AGE-BSA) fluorescence versus BSA. The assay can distinguish both species (AGEs and non-oxidized proteins).
- FuncS
Supplier Data
Functional Studies - Advanced Glycation End Products (AGEs) Assay Kit (AB273298)
AGE activity in serum samples.
AGEs amount in Pooled Human Serum from healthy donors (4 μg serum protein), Human Diabetic Serum (4 μg serum protein) and Human Urine from healthy donor.
Complementary Products
Product details
Advanced Glycation End Products (AGEs) Activity Assay Kit (ab273298) can be used for the semi-quantitative estimation of fluorescent AGEs levels in Biological Fluids. The assay is based on the characteristic fluorescence (Ex/Em= 360/460 nm), a characteristic that is shared by almost all AGEs. The proprietary composition of the Assay Buffer specifically distinguishes between AGEs and non-oxidized proteins. The one-step assay uses oxidized Bovine Serum Albumin (AGE-BSA) as a Positive Control. BSA is used as Background Control and its fluorescence under assay conditions is defined as 1 relative fluorescence intensity in arbitrary units (AU or RFUSample/ RFUBackground).
This product is manufactured by BioVision, an Abcam company and was previously called K929 Advanced Glycation End Products (AGEs) Assay Kit. K929-100 is the same size as the 100 test size of ab273298.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.
Pathways
AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.
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Publications (5)
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Medicina (Kaunas, Lithuania) 61: PubMed40572656
2025
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Nutrients 16: PubMed39339746
2024
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BMC complementary medicine and therapies 24:225 PubMed38858747
2024
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BMC cardiovascular disorders 23:446 PubMed37697234
2023
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International journal of molecular sciences 24: PubMed36675183
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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