AB238539

AGE (Advanced Glycation End) Assay Kit

Name: AGE (Advanced Glycation End) Assay Kit (ab238539) | Abcam
Brand: Abcam Limited
SKU: AB238539
Price: 930 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(8 Publications)

AGE (Advanced Glycation End Products) Assay Kit (ab238539) is designed for the rapid detection and quantitation of advanced glycation end product protein adducts.

View Alternative Names

AGEs, Advanced glycation end products

2 Images

Competitive ELISA - AGE (Advanced Glycation End) Assay Kit (AB238539)

cELISA

Supplier Data

Competitive ELISA - AGE (Advanced Glycation End) Assay Kit (AB238539)

Advanced Glycation End Product Formation Pathways.

CML = N-epsilon-(carboxymethyl)lysine.

GOLD = glyoxal-derived lysine dimer.

CEL = N-epsilon-(1-carboxyethyl)lysine.

MOLD = methylglyoxal-derived lysine dimer.

DOLD = 3-deoxyglucosone-derived lysine dimer.

3-DG = 3-deoxyglucosone.

cELISA

Supplier Data

Competitive ELISA - AGE (Advanced Glycation End) Assay Kit (AB238539)

Example AGE-BSA Competitive ELISA Standard Curve.

Typical AGE Competitive ELISA results.

This data is for reference only and should not be used to interpret actual results.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Plasma, Serum, Cell Lysate

Assay type

Competitive

Sensitivity

= 0.5 µg/mL

Range

0.36 - 100 µg/mL

Assay Platform

Microplate reader

style

left-column

Product details

AGE (Advanced Glycation End Products) Assay Kit (ab238539) is designed for the rapid detection and quantitation of advanced glycation end product protein adducts.

Advanced Glycation End Products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.

style

left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab238539-96Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"AGE Conjugate", "number":"AB238539-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Diluent", "number":"AB238539-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Substrate Solution", "number":"AB238539-CMP10", "productcode":"" }, { "size":"1 x 125 µL", "name":"AGE-BSA Standard", "number":"AB238539-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Protein Binding Strip Well Plate", "number":"AB238539-CMP03", "productcode":"" }, { "size":"1 x 20 µL", "name":"Secondary Antibody, HRP Conjugate (1000X)", "number":"AB238539-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Wash Buffer", "number":"AB238539-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Anti-AGE Antibody (1000X)", "number":"AB238539-CMP06", "productcode":"" }, { "size":"1 x 300 µL", "name":"100X Conjugate Diluent", "number":"AB238539-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238539-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Advanced Glycation End-products (AGEs) are highly reactive compounds formed when reducing sugars non-enzymatically bind to proteins lipids or nucleic acids. They are often referred to as AGE products or AGE-BSA when in the form of bovine serum albumin (BSA). The molecular weight of AGE compounds can vary significantly due to the diversity of glycation products. AGEs express in various tissues but accumulate more in aging tissues and in certain pathological states. Detection of AGE products often utilizes AGE ELISA assays or fluorescence-based methods to measure AGE-BSA.

Biological function summary

AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.

Pathways

AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.

AGE compounds play significant roles in the progression of diabetes and cardiovascular disease. In diabetes AGEs contribute to complications by modifying vascular structure and function where they link with proteins such as HbA1c in glycation. In cardiovascular disease AGE accumulation in vascular tissues enhances vascular stiffness and atherosclerotic lesion formation. The AGE-RAGE interaction perpetuates chronic inflammation further exacerbating these conditions.

style

left-column

Product protocols

style

left-column

Target data

See full target information AGE

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23515 PubMed37632267

2023

Advanced glycation end products induce nucleus pulposus cell apoptosis by upregulating TXNIP via inhibiting glycolysis pathway in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Chen,Xiaoping Sheng,Haobo Sun,Qunfeng Guo,Haibin Wang,Lecheng Wu,Bin Ni,Jun Yang

Biomolecules 13: PubMed37371535

2023

Emergent Inflammatory Markers and Echocardiographic Indices in Patients with Bronchial Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolaos A Gkavogiannakis,James N Tsoporis,Ioannis-Alexandros Drosatos,George Tsirebolos,Shehla Izhar,Eleftherios Sakadakis,Andreas S Triantafyllis,Thomas G Parker,Lampros A Kalogiros,Howard Leong-Poi,Loukianos S Rallidis,Ioannis Rizos

Diabetology & metabolic syndrome 14:99 PubMed35841066

2022

MiR-574-3p inhibits glucose toxicity-induced pancreatic β-cell dysfunction by suppressing PRMT1.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Lv,Xiumin Wang,Jinhua Shen,Ying Cao,Qin Zhang

BMC oral health 22:206 PubMed35614406

2022

Increased expression of advanced glycation endproducts in the gingival crevicular fluid compromises periodontal status in cigarette-smokers and waterpipe users.

Applications

Unspecified application

Species

Unspecified reactive species

Dena Ali,Fatemah AlAhmari,Toshinari Mikami,Jagan Kumar Baskaradoss

Molecular medicine reports 25: PubMed35211757

2022

Advanced glycation end products promote osteoporosis by inducing ferroptosis in osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Ge,Jian Jie,Jie Yao,Wei Li,Yahui Cheng,Wenjuan Lu

Biomedical reports 16:28 PubMed35251615

2022

Serum advanced glycation end-products and αB-crystallin in diabetic retinopathy patients.

Applications

Unspecified application

Species

Unspecified reactive species

Taku Yamamoto,Satoru Kase,Miyuki Murata,Susumu Ishida

Journal of Alzheimer's disease : JAD 78:989-1010 PubMed33074237

2020

Benfotiamine and Cognitive Decline in Alzheimer's Disease: Results of a Randomized Placebo-Controlled Phase IIa Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gary E Gibson,José A Luchsinger,Rosanna Cirio,Huanlian Chen,Jessica Franchino-Elder,Joseph A Hirsch,Lucien Bettendorff,Zhengming Chen,Sarah A Flowers,Linda M Gerber,Thomas Grandville,Nicole Schupf,Hui Xu,Yaakov Stern,Christian Habeck,Barry Jordan,Pasquale Fonzetti

Journal of periodontology 91:396-402 PubMed31389020

2019

Levels of advanced glycation end products in gingival crevicular fluid of chronic periodontitis patients with and without type-2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Zohaib Akram,Fawaz Alqahtani,Mana Alqahtani,Abdulaziz A Al-Kheraif,Fawad Javed

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

AGE (Advanced Glycation End) Assay Kit

Competitive ELISA - AGE (Advanced Glycation End) Assay Kit (AB238539)

Competitive ELISA - AGE (Advanced Glycation End) Assay Kit (AB238539)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Product promise