Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.

Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575

• Biochemical assay

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Biochemical assay - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Diagram showing the principles of the ALT assay method.

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Colorimetric standard curve : mean of duplicates (+/- SD) with background reads subtracted.

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Pyruvate measured in biological fluids after 20 min and 40 min incubation time showing quantity (nmol) per ml of tested sample.

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Functional Studies - Alanine Transaminase Activity Assay Kit (105134).

Pyruvate measured fluorometrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Measurement of alanine transaminase in HepG2 cells (10 μg) and liver lysate (15 μg).

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PubMed

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Liver samples from high fat diet (HFD) and standard carbohydrate diet (CHD) BALB/c and C57BL6/J mice were homogenised in an ALT assay buffer for the determination of ALT activity using ab105134. A separate batch of liver extracts was prepared and incubated in a buffer containing NP40 (5%) and supernatants containing the triglycerides were separated. Triglycerides concentration was determined on the supernatant fraction using ab65336. ALT activity and triglycerides concentration were determined by measuring OD at 570nm.

Waller-Evans H et al., PLoS One, 8(12). Fig 1d. doi: 10.1371/journal.pone.0082825 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.

• FuncS

Lab

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Pyruvate measured in mouse tissue lysates after 20 min and 40 min incubation time showing quantity (nmol) per mg of tested sample.

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Lab

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Fluorometric standard curve : mean of duplicates (+/- SD) with background reads subtracted.

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Lab

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.

Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.

• Enzyme activity assay

PubMed

Enzyme activity assay - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Alanine Aminotransferase assay kit ab105134 and Aspartate Aminotransferase assay kit ab105135 used with mouse serum.

Hwang et al used Alanine Aminotransferase assay kit ab105134 and Aspartate Aminotransferase assay kit ab105135 to investigate the impact of endothelial-specific-TAZ-knockout of mice fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) to induce fibrosis.

Partial hepatectomy (PHx) was conducted in WT and eKO mice, following which the livers were subjected to Sirius Red staining to visualize fibrosis, at 8 d after PHx. Provided data are representative images from multiple mice. The stained area was measured and analyzed using ImageJ (n = 4). Serum of each mouse was analyzed to determine the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity (n = 8 for ALT and n = 6 for AST).

Mouse serum was prepared by collecting blood by means of tail bleeding, followed by centrifugation after blood clotting, and incubated at room temperature for 30 min. The supernatant was collected and directly assessed using an Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (K752, BioVision, now manufactured by Abcam as ab105134). The subsequent experimental steps were performed according to the manufacturer's protocol.

Aspartate aminotransferase (AST) activity assay : Mouse serum was prepared by tail bleeding. The isolated serum was assayed directly using an aspartate aminotransferase (AST) activity assay kit (ab105135, Abcam). Subsequent experimental steps were performed according to the manufacturer's protocol.

• Schematic Diagram

Supplier Data

Schematic Diagram - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

Representative image of Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134

Components shown from left to right :

- OxiRed Probe

- ALT Positive Control

- Pyruvate Standard

- ALT Substrate Mix

- Developer Mix A

- Assay Buffer 13

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.