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AB235628

Albumin (BCG) Assay Kit (Colorimetric)

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(7 Publications)

Albumin (BCG) Assay Kit (Colorimetric) (ab235628): add serum sample, add buffer / reagent, incubate for 20-min at room temp. Readout on colorimetric plate reader (620 nm).

View Alternative Names

Albumin, BSA, ALB

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Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)
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Supplier Data

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

BSA Standard Curve (0-75 μg).

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)
  • FuncS

Supplier Data

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

Albumin concentration in pooled human serum (PHS), fetal bovine serum (FBS) and bovine calf serum (BCS).

Sample volumes (0-20 μL) were assayed following the kit protocol. Albumin concentration (g/dL) : PHS : 3.8 ± 0.4; FBS : 1.6 ± 0.1; BCS : 2.6 ± 0.5.

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)
  • FuncS

Supplier Data

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

BSA Standard Curve (0-500 μg).

Key facts

Detection method

Colorimetric

Sample types

Serum

Assay type

Quantitative

Sensitivity

= 5 µg

Assay Platform

Microplate reader

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Product details

Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.

Albumin BCG assay method
The BCG albumin assay method is based on the selective interaction between Bromocresol Green (BCG) and albumin forming a chromophore that can be detected at 620 nm. The signal is directly proportional to the amount of albumin present in the serum. BCG does not react with other abundant plasma proteins like IgG.

The assay can detect as low as 5 μg (0.01 g/dL) of albumin in serum samples.

Albumin BCG assay protocol summary
- add undiluted serum to 96-well plate
- add assay buffer to adjust final volume to 50 μL/well
- add reaction mix
- incubate for 20-min
- analyze on plate reader

Other notes
This product was previously called K554 Biovison Albumin (BCG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Albumin is the most abundant protein in human blood and is highly conserved among vertebrates. It plays a pivotal physiological role in maintenance of plasma osmotic pressure, vascular permeability, and transport of cholesterol, bile pigments, nitric oxide, metals, and other small molecules in the body. It also functions as a free radical scavenger of reactive oxygen and nitrogen species, triggers cell signaling processes, possesses anti-inflammatory and coagulatory effects.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab235628-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Bromocresol Green", "number":"AB235628-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Albumin Assay Buffer", "number":"AB235628-CMP01", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"BSA Standard I", "number":"AB235628-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information Albumin

Additional targets

Albumin
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 4:102372 PubMed37352106

2023

Quantitative cytofluorimetric analysis of mouse neutrophil extracellular traps.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Poli,Marco Di Gioia,Ivan Zanoni

Cell reports 42:112064 PubMed36724077

2023

Staphylococcus aureus stimulates neutrophil itaconate production that suppresses the oxidative burst.

Applications

Unspecified application

Species

Unspecified reactive species

Kira L Tomlinson,Sebastián A Riquelme,Swikrity Upadhyay Baskota,Marija Drikic,Ian R Monk,Timothy P Stinear,Ian A Lewis,Alice S Prince

International journal of molecular sciences 23: PubMed36077183

2022

Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Nora A Spraakman,Annemieke M Coester,Arno R Bourgonje,Vincent B Nieuwenhuijs,Jan-Stephan F Sanders,Henri G D Leuvenink,Harry van Goor,Gertrude J Nieuwenhuijs-Moeke

iScience 24:103256 PubMed34761180

2021

Zinc-dependent histone deacetylases drive neutrophil extracellular trap formation and potentiate local and systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Poli,Victor Pui-Yan Ma,Marco Di Gioia,Achille Broggi,Mehdi Benamar,Qian Chen,Ralph Mazitschek,Stephen J Haggarty,Talal A Chatila,Jeffrey M Karp,Ivan Zanoni

Animals : an open access journal from MDPI 11: PubMed34208851

2021

Seeds: A Potential Natural Immune Modulator Source for Broiler Reared under Chronic Heat Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed I Alzarah,Fayez Althobiati,Ahmed O Abbas,Gamal M K Mehaisen,Nancy N Kamel

Metallomics : integrated biometal science 11:1805-1819 PubMed31612889

2019

A metalloproteomic analysis of interactions between plasma proteins and zinc: elevated fatty acid levels affect zinc distribution.

Applications

Unspecified application

Species

Unspecified reactive species

James P C Coverdale,James P Barnett,Adamu H Adamu,Ellie J Griffiths,Alan J Stewart,Claudia A Blindauer

Artificial cells, nanomedicine, and biotechnology 47:3212-3221 PubMed31359793

2019

Green synthesis of silver nanoparticles from mitigates cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Zhang,Guangda Xin,Guangyu Zhou,Qianyu Li,Vishnu Priya Veeraraghavan,Surapaneni Krishna Mohan,Dayu Wang,Feng Liu
View all publications
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