Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- STAR protocols 4:1023722023Quantitative cytofluorimetric analysis of mouse neutrophil extracellular traps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Poli,Marco Di Gioia,Ivan ZanoniPubMed 37352106
- Cell reports 42:1120642023Staphylococcus aureus stimulates neutrophil itaconate production that suppresses the oxidative burst.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKira L Tomlinson,Sebastián A Riquelme,Swikrity Upadhyay Baskota,Marija Drikic,Ian R Monk,Timothy P Stinear,Ian A Lewis,Alice S PrincePubMed 36724077
- International journal of molecular sciences 23:2022Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNora A Spraakman,Annemieke M Coester,Arno R Bourgonje,Vincent B Nieuwenhuijs,Jan-Stephan F Sanders,Henri G D Leuvenink,Harry van Goor,Gertrude J Nieuwenhuijs-MoekePubMed 36077183
- iScience 24:1032562021Zinc-dependent histone deacetylases drive neutrophil extracellular trap formation and potentiate local and systemic inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Poli,Victor Pui-Yan Ma,Marco Di Gioia,Achille Broggi,Mehdi Benamar,Qian Chen,Ralph Mazitschek,Stephen J Haggarty,Talal A Chatila,Jeffrey M Karp,Ivan ZanoniPubMed 34761180
- Animals : an open access journal from MDPI 11:2021Seeds: A Potential Natural Immune Modulator Source for Broiler Reared under Chronic Heat Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed I Alzarah,Fayez Althobiati,Ahmed O Abbas,Gamal M K Mehaisen,Nancy N KamelPubMed 34208851
- Metallomics : integrated biometal science 11:1805-18192019A metalloproteomic analysis of interactions between plasma proteins and zinc: elevated fatty acid levels affect zinc distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames P C Coverdale,James P Barnett,Adamu H Adamu,Ellie J Griffiths,Alan J Stewart,Claudia A BlindauerPubMed 31612889
- Artificial cells, nanomedicine, and biotechnology 47:3212-32212019Green synthesis of silver nanoparticles from mitigates cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiping Zhang,Guangda Xin,Guangyu Zhou,Qianyu Li,Vishnu Priya Veeraraghavan,Surapaneni Krishna Mohan,Dayu Wang,Feng LiuPubMed 31359793
Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Quantitative
- Reactive species
Mammals
- Sensitivity
= 5 µg
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Targets
ALB
Alternative names
Albumin, BSA, ALB
What's included?
Recommended products
Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Albumin, BSA, ALB
Key facts
- Detection method
Colorimetric
- Sample types
Serum
- Assay type
Quantitative
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
= 5 µg
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.
The assay is based on the selective interaction between Bromocresol Green (BCG) and albumin forming a chromophore that can be detected at 620 nm. The signal is directly proportional to the amount of albumin present in the serum. BCG does not react with other abundant plasma proteins like IgG.
The assay can detect as low as 5 μg (0.01 g/dL) of albumin in serum samples.
This product is manufactured by BioVision, an Abcam company and was previously called K554 Albumin (BCG) Assay Kit (Colorimetric). K554-100 is the same size as the 100 test size of ab235628.
Albumin is the most abundant protein in human blood and is highly conserved among vertebrates. It plays a pivotal physiological role in maintenance of plasma osmotic pressure, vascular permeability, and transport of cholesterol, bile pigments, nitric oxide, metals, and other small molecules in the body. It also functions as a free radical scavenger of reactive oxygen and nitrogen species, triggers cell signaling processes, possesses anti-inframmatory and coagulatory effects.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (ab235628)
Albumin concentration in pooled human serum (PHS), fetal bovine serum (FBS) and bovine calf serum (BCS).
Sample volumes (0-20 μL) were assayed following the kit protocol. Albumin concentration (g/dL): PHS: 3.8 ± 0.4; FBS: 1.6 ± 0.1; BCS: 2.6 ± 0.5.
Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (ab235628)
BSA Standard Curve (0-75 μg).
Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (ab235628)
BSA Standard Curve (0-500 μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com