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AB241018

Albumin Creatinine Ratio Assay Kit

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(5 Publications)

Albumin Creatinine Ratio Assay Kit (ab241018) combines a fluorometric albumin assay and a colorimetric creatinine assay in one convenient kit. Use with urine, saliva, serum.

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

3 Images
Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)
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Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)

Creatinine Standard Curve.

Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)
  • FuncS

Unknown

Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)

BSA Standard Curve.

Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)
  • FuncS

Unknown

Functional Studies - Albumin Creatinine Ratio Assay Kit (AB241018)

Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3).

For Albumin, 50 μL of undiluted samples and for Creatinine, 30 μL of diluted samples (100 times diluted using Creatinine Assay Buffer) were assayed following the kit protocol.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Serum

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Product details

The Albumin Creatinine Ratio Assay Kit (ab241018) provides a simple, sensitive, and high throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/mL), creatinine (detection range: 0.002 -0.5 mg/mL) and Albumin-to-creatinine ratio.

The ACR ratio is determined in two steps: First, albumin is determined by using a probe (Albumin Blue 580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Arcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.

Other Notes
This product was previously called K551 Biovision Albumin-to-Creatinine Ratio (ACR) Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241018-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Creatininase", "number":"AB241018-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 34", "number":"AB241018-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Albumin Diluent", "number":"AB241018-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Albumin Assay Buffer II", "number":"AB241018-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"BSA Standard II", "number":"AB241018-CMP10", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB241018-CMP08", "productcode":"" }, { "size":"1 x 400 µL", "name":"Albumin Probe", "number":"AB241018-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Creatinine Standard", "number":"AB241018-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Sarcosine Enzyme Mix", "number":"AB241018-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Creatinase", "number":"AB241018-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin/Creatinine often abbreviated as the albumin to creatinine ratio (ACR) serves as an important marker in the body. Albumin is a plasma protein with a molecular weight of approximately 66.5 kDa. It is synthesized in the liver and widely expressed in the blood while creatinine a breakdown product of creatine phosphate is a small molecule produced in the muscles and excreted in urine. The albumin/creatinine ratio measures these levels in urine to assess excretion balance and renal function.
Biological function summary

The albumin and creatinine levels help evaluate kidney function and filtration efficiency. Albumin signifies the presence of larger proteins in urine when normal kidney filtering mechanisms are impaired. The presence and concentration of creatinine meanwhile reflect waste removal efficacy. The albumin/creatinine ratio provides a reliable estimation of microalbuminuria which is an elevation in urinary albumin considered indicative of early kidney damage. This ratio is not directly part of a complex but offers insight into the normal physiological role of kidneys.

Pathways

Albumin/creatinine measurements relate primarily to renal regulation and excretory pathways. They indicate how well glomeruli the filtering units in kidneys function relative to proteins and byproducts' reabsorption or excretion. These measurements tie to pathways involving renin-angiotensin-aldosterone system and vasopressin signaling with contacts to proteins like nephrin and podocin that maintain the integrity of the glomerular filtration barrier.

Albumin/creatinine ratios possess strong correlations with conditions such as diabetes and hypertension. High levels of this ratio suggest an increased risk for diabetic nephropathy where persistent albuminuria develops as the disease progresses. In hypertension albuminuria signifies the strain and damage on kidney structures often exacerbating chronic kidney disease. This ratio is additionally linked with angiotensin-converting enzyme (ACE) frequently altered in these conditions to manage blood pressure and minimize kidney damage.
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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Kidney360 6:38-48 PubMed39621430

2024

Dynll1-PI31 Interaction Enhances Proteolysis Through the Proteasome, Representing a Novel Therapeutic Target for INF2-Related FSGS.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian Williquett,Chandra Perez-Gill,Chantal Allamargot,Faith Rooney,Martin R Pollak,Hua Sun

Cell biochemistry and function 42:e3943 PubMed38379015

2024

Integrative ATAC-seq and RNA-seq analysis associated with diabetic nephropathy and identification of novel targets for treatment by dapagliflozin.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxiao Shen,Liang Ying,Jiajia Wu,Yan Fang,Wenyan Zhou,Chaojun Qi,Leyi Gu,Shan Mou,Yuru Yan,Ming Tian,Zhaohui Ni,Xiajing Che

Frontiers in immunology 14:1205834 PubMed38022502

2023

Dapagliflozin alleviates renal inflammation and protects against diabetic kidney diseases, both dependent and independent of blood glucose levels.

Applications

Unspecified application

Species

Unspecified reactive species

Anxiang Cai,Jianxiao Shen,Xiaoqian Yang,Xinghua Shao,Leyi Gu,Shan Mou,Xiajing Che

International journal of molecular sciences 24: PubMed37628958

2023

Metalloporphyrins Reduce Proteinuria in Podocyte Immune Injury: The Role of Metal and Porphyrin Moieties.

Applications

Unspecified application

Species

Unspecified reactive species

Elias A Lianos,Gia Nghi Phung,Michelle Foster,Jianping Zhou,Mukut Sharma

NPJ Regenerative medicine 7:15 PubMed35149726

2022

Telomerase is required for glomerular renewal in kidneys of adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Margo Montandon,Tynhinane Hamidouche,Lucile Yart,Lou C Duret,Catherine Pons,Nicolas Soubeiran,Mélanie Pousse,Ludovic Cervera,Valérie Vial,Julien Fassy,Olivier Croce,Eric Gilson,Marina Shkreli
View all publications
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websiteProtocolBooklet
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