The Alcian Blue (pH 1.0) (Mucin Stain) is intended for use in the histological visualization of strongly sulfated mucosubstances.
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- Bioengineering (Basel, Switzerland) 9:2022Heparan Sulfate Glycosaminoglycan Is Predicted to Stabilize Inflammatory Infiltrate Formation and RANKL/OPG Ratio in Severe Periodontitis in Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoko Duplancic,Marija Roguljic,Darko Bozic,Darko KeroPubMed 36290535
- Nature communications 12:2702021CREPT is required for murine stem cell maintenance during intestinal regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiu Yang et. al.PubMed 33431892
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The Alcian Blue (pH 1.0) (Mucin Stain) is intended for use in the histological visualization of strongly sulfated mucosubstances.
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- Appropriate long-term storage conditions
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Notes
The Alcian Blue (pH 1.0) (Mucin Stain) is intended for use in the histological visualization of strongly sulfated mucosubstances.
The component Acetic Acid Solution has been replaced with Hydrochloric Acid Solution in this kit.
Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- prepare Working Rinse solution
- apply Working Rinse solution to tissue for 30 sec
- incubate in Alcian Blue pH 1.0 for 30 min
- rinse in Working Rinse Solution
- blot and air dry slide
- stain in nuclear fast red for 1-2 min
- rinse in water and allow to dry completely
- clear and mount
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Staining Interpretation| Strongly Sulfated Mucosubstances | Blue |
| Nuclei | Red |
| Background | Pink |
Control Tissue: Small Intestine, Appendix, Colon
Previously called Alcian Blue pH 1.0 Stain Kit (Mucin Stain)
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 1.0, Mucin Stain) (ab150661)
ab150661 Alcian Blue pH 1 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 1.0, Mucin Stain) (ab150661)
ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, counterstained with safranin o (pink).
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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