Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is used to stain tissue sections for sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
- Suitable for formalin-fixed paraffin-embedded sections
- Cited in over 30 publications
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- Journal of neuroinflammation 20:2822023Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamkwon Kim,In Gyoung Ju,Seung Ho Jeon,Yeongae Lee,Min-Ji Jung,Min Sung Gee,Jae Seok Cho,Kyung-Soo Inn,Lee Ann Garrett-Sinha,Myung Sook Oh,Jong Kil LeePubMed 38012646
- JMIR research protocols 12:e429642023Efficient Generation of Chondrocytes From Bone Marrow-Derived Mesenchymal Stem Cells in a 3D Culture System: Protocol for a Practical Model for Assessing Anti-Inflammatory Therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajashree Patnaik,Shirin Jannati,Bala Mohan Sivani,Manfredi Rizzo,Nerissa Naidoo,Yajnavalka BanerjeePubMed 37505889
- Nature communications 14:40052023TET2 and TET3 loss disrupts small intestine differentiation and homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIhab Ansari,Llorenç Solé-Boldo,Meshi Ridnik,Julian Gutekunst,Oliver Gilliam,Maria Korshko,Timur Liwinski,Birgit Jickeli,Noa Weinberg-Corem,Michal Shoshkes-Carmel,Eli Pikarsky,Eran Elinav,Frank Lyko,Yehudit BergmanPubMed 37414790
- Journal of functional biomaterials 14:2023Preliminary In Vitro Assessment of Decellularized Porcine Descending Aorta for Clinical Purposes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Casarin,Tiago Moderno Fortunato,Saima Jalil Imran,Martina Todesco,Deborah Sandrin,Massimo Marchesan,Gino Gerosa,Filippo Romanato,Andrea Bagno,Fabrizio Dal Moro,Alessandro MorlaccoPubMed 36976065
- Kidney international reports 8:837-8502023The Transcriptomics of the Human Vein Transformation After Arteriovenous Fistula Anastomosis Uncovers Layer-Specific Remodeling and Hallmarks of Maturation Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaisel Martinez,Miguel G Rojas,Marwan Tabbara,Simone Pereira-Simon,Nieves Santos Falcon,Mohd Ahmar Rauf,Akshara Challa,Zachary M Zigmond,Anthony J Griswold,Juan C Duque,Roberta M Lassance-Soares,Omaida C Velazquez,Loay H Salman,Roberto I Vazquez-PadronPubMed 37069981
- Disease models & mechanisms 16:2023Induction of pancreatic neoplasia in the KRAS/TP53 Oncopig.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPinaki Mondal,Neesha S Patel,Katie Bailey,Shruthishree Aravind,Sara B Cartwright,Michael A Hollingsworth,Audrey J Lazenby,Mark A CarlsonPubMed 36579622
- Gut microbiome (Cambridge, England) 3:2022Impact of gut permeability on the breast microbiome using a non-human primate model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlaa Bawaneh,Carol A Shively,Janet Austin Tooze,Katherine Loree CookPubMed 36891249
- Cancers 14:2022Intestinal Microbiota Influence Doxorubicin Responsiveness in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlaa Bawaneh,Adam S Wilson,Nicole Levi,Marissa M Howard-McNatt,Akiko Chiba,David R Soto-Pantoja,Katherine L CookPubMed 36230772
- Cell reports 40:1114292022Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L GibbonsPubMed 36170810
- Gut and liver :2022Novel Histone Deacetylase 6 Inhibitor Confers Anti-inflammatory Effects and Enhances Gut Barrier Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae-Young Lee,Hyun Woo Ma,Ji Hyung Kim,I Seul Park,Mijeong Son,Keun Ho Ryu,Jieun Shin,Seung Won Kim,Jae Hee CheonPubMed 36167345
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Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is used to stain tissue sections for sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
- Suitable for formalin-fixed paraffin-embedded sections
- Cited in over 30 publications
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.
Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- incubate in acetic acid for 3 min
- incubate in Alcian Blue pH 2.5 for 30 min
- rinse in water
- stain in nuclear fast red for 5 min
- rinse in water
- dehydrate, clear and mount
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Staining Interpretation| Acidic Sulfated Mucosubstances | Blue |
| Hyaluronic Acid | Blue |
| Sialomucins | Blue |
| Nuclei | Red |
| Background | Pink |
Control Tissue: Small Intestine, Appendix, Colon
Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)
ab150662 (Alcian Blue pH2.5 stain) staining acidic sulphated mucins (blue) in formalin fixed paraffin embedded normal human colon, counterstained with safranin o (pink).
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)
ab150662 Alcian Blue pH 2.5 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)
Staining of Colon using ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain).
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