Skip to main content

Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated, acid mucopolysaccharides and sialomucins.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662), expandable thumbnail

Publications

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Acetic Acid Solution (3%)
1 x 500 mL
Alcian Blue pH 2.5
1 x 250 mL
Nuclear Fast Red Solution
1 x 250 mL

Recommended products

Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated, acid mucopolysaccharides and sialomucins.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.

Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- incubate in acetic acid for 3 min
- incubate in Alcian Blue pH 2.5 for 30 min
- rinse in water
- stain in nuclear fast red for 5 min
- rinse in water
- dehydrate, clear and mount

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

Acidic Sulfated Mucosubstances Blue
Hyaluronic AcidBlue
SialomucinsBlue
NucleiRed
BackgroundPink

Control Tissue: Small Intestine, Appendix, Colon

Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)

    ab150662 Alcian Blue pH 2.5 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)

    ab150662 (Alcian Blue pH2.5 stain) staining acidic sulphated mucins (blue) in formalin fixed paraffin embedded normal human colon, counterstained with safranin o (pink).

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)

    Staining of Colon using ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com