AB150662

Alcian Blue Stain Kit (pH 2.5, Mucin Stain)

Name: Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662) | Abcam
Brand: Abcam Limited
SKU: AB150662
Price: 95 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(51 Publications)

Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is used to stain tissue sections for sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

- Suitable for formalin-fixed paraffin-embedded sections
- Cited in over 30 publications

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

ab150662 (Alcian Blue pH2.5 stain) staining acidic sulphated mucins (blue) in formalin fixed paraffin embedded normal human colon, counterstained with safranin o (pink).

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

Staining of Colon using ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

ab150662 Alcian Blue pH 2.5 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.

Key facts

style

left-column

style

left-column

Product details

Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.

Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- incubate in acetic acid for 3 min
- incubate in Alcian Blue pH 2.5 for 30 min
- rinse in water
- stain in nuclear fast red for 5 min
- rinse in water
- dehydrate, clear and mount

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

Acidic Sulfated Mucosubstances	Blue
Hyaluronic Acid	Blue
Sialomucins	Blue
Nuclei	Red
Background	Pink

Control Tissue: Small Intestine, Appendix, Colon

Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)

Other Notes

Please note, the 250 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

style

left-column

What's included?

{ "values": { "250mL": { "sellingSize": "250 mL", "publicAssetCode":"ab150662-250mL", "assetComponentDetails": [ { "size":"1 x 250 mL", "name":"Alcian Blue pH 2.5", "number":"AB150662-CMP02" }, { "size":"1 x 500 mL", "name":"Acetic Acid Solution (3%)", "number":"AB150662-CMP01" }, { "size":"1 x 250 mL", "name":"Nuclear Fast Red Solution", "number":"AB150662-CMP03" } ] }, "30mL": { "sellingSize": "30 mL", "publicAssetCode":"ab150662-30mL", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"Acetic Acid Solution (3%)", "number":"AB150662-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Alcian Blue pH 2.5", "number":"AB150662-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Nuclear Fast Red Solution", "number":"AB150662-CMP03", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Ambient

style

left-column

style

left-column

Product protocols

style

left-column

Target data

See full target information Alcian-blue

style

left-column

Publications (51)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0310485 PubMed40569981

2025

Nebulized and intravenous enzyme replacement therapy in mice with mucopolysaccharidosis type II.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J Shamoun,Gisienne Reis,Malaica Ashley,Anatalia Labilloy,Leonardo F Ferreira

Journal of translational medicine 23:376 PubMed40148953

2025

Circ-ITCH promotes the ubiquitination degradation of HOXC10 to facilitate osteogenic differentiation in disuse osteoporosis through stabilizing BRCA1 mRNA via IGF2BP2-mediated mA modification.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhong,Xi Li,Zhen Yin,Peng Chen,Yusheng Li,Jian Tian,Long Wang,Hua Liu,Ke Yin,Lemei Zhu,Lingyu Kong,Kunli Chen,Yaochun Li,Chungu Hong,Chenggong Wang

PloS one 20:e0317056 PubMed39775680

2025

Mesothelial cell responses to acute appendicitis or small bowel obstruction reactive ascites: Insights into immunoregulation of abdominal adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa A Hausburg,Kaysie L Banton,Christopher D Cassidy,Robert M Madayag,Carlos H Palacio,Jason S Williams,Raphael Bar-Or,Rebecca J Ryznar,David Bar-Or

JCI insight 10: PubMed39739419

2025

EZH2 deletion does not affect acinar regeneration but restricts progression to pancreatic cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Jaune-Pons,Xiaoyi Wang,Fatemeh Mousavi,Zachary Klassen,Abdessamad El Kaoutari,Kurt Berger,Charis Johnson,Mickenzie B Martin,Saloni Aggarwal,Sukhman Brar,Muhammad Khalid,Joanna F Ryan,Parisa Shooshtari,Angela J Mathison,Nelson Dusetti,Raul Urrutia,Gwen Lomberk,Christopher L Pin

Biomaterials science 12:6284-6298 PubMed39415619

2024

Wharton's jelly of the umbilical cord serves as a natural biomaterial to promote osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Show Fu,Shang-Wen Tsai,Zhen-Jie Tong,Chang-Ching Yeh,Tien-Hua Chen,Cheng-Fong Chen

Frontiers in cardiovascular medicine 11:1398114 PubMed39355352

2024

Cellular and extracellular proteomic profiling of paradoxical low-flow low-gradient aortic stenosis myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Manar Elkenani,Javier Barallobre-Barreiro,Moritz Schnelle,Belal A Mohamed,Bo E Beuthner,Christoph Friedemann Jacob,Niels B Paul,Xiaoke Yin,Konstantinos Theofilatos,Andreas Fischer,Miriam Puls,Elisabeth M Zeisberg,Ajay M Shah,Manuel Mayr,Gerd Hasenfuß,Karl Toischer

Materials today. Bio 28:101228 PubMed39296356

2024

Development of bioactive solid-foam scaffolds from decellularized cartilage with chondrogenic and osteogenic properties.

Applications

Unspecified application

Species

Unspecified reactive species

Unai Mendibil,Yaiza Lópiz-Morales,Blanca Arnaiz,Raquel Ruiz-Hernández,Pablo Martín,Desiré Di-Silvio,Nerea Garcia-Urquia,Felix Elortza,Mikel Azkargorta,Beatriz Olalde,Ander Abarrategi

Cell reports. Medicine 5:101711 PubMed39232498

2024

Loss of p53 and SMAD4 induces adenosquamous subtype pancreatic cancer in the absence of an oncogenic KRAS mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Daowei Yang,Xinlei Sun,Rohan Moniruzzaman,Hua Wang,Citu Citu,Zhongming Zhao,Ignacio I Wistuba,Huamin Wang,Anirban Maitra,Yang Chen

Journal of clinical medicine 13: PubMed39200916

2024

Exploring the Potential of Saphenous Vein Grafts Ex Vivo: A Model for Intimal Hyperplasia and Re-Endothelialization.

Applications

Unspecified application

Species

Unspecified reactive species

Nur A'tiqah Haron,Mohamad Fikeri Ishak,Muhammad Dain Yazid,Ubashini Vijakumaran,Roszita Ibrahim,Raja Zahratul Azma Raja Sabudin,Hafiza Alauddin,Nur Ayub Md Ali,Hairulfaizi Haron,Muhammad Ishamuddin Ismail,Mohd Ramzisham Abdul Rahman,Nadiah Sulaiman

Cardiovascular research 120:1851-1868 PubMed39167826

2024

Smooth muscle-specific deletion of cellular communication network factor 2 causes severe aorta malformation and atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jannik H Larsen,Julie S Hegelund,Matilde K Pedersen,Cecilie M Andersson,Caroline A Lindegaard,Didde R Hansen,Jane Stubbe,Jes S Lindholt,Camilla S Hansen,Andrietta Grentzmann,Maria Bloksgaard,Boye L Jensen,Raúl R Rodriguez-Díez,Marta Ruiz-Ortega,Sebastian Albinsson,Gerard Pasterkamp,Michal Mokry,Andrew Leask,Roel Goldschmeding,Bartosz Pilecki,Grith L Sorensen,Charles Pyke,Martin Overgaard,Hans C Beck,Daniel F J Ketelhuth,Lars M Rasmussen,Lasse B Steffensen

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alcian Blue Stain Kit (pH 2.5, Mucin Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

Key facts

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (51)

Product promise