JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB102533

Alcohol Dehydrogenase Assay Kit

5

(1 Review)

|

(19 Publications)

Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples.

View Alternative Names

ADH3, ADH1C, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma, ADHX, FDH, ADH5, Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase, FALDH, GSH-FDH, All-trans-retinol dehydrogenase [NAD(+)] ADH7, Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Omega-hydroxydecanoate dehydrogenase ADH7, Retinol dehydrogenase, ADH7, ADH1, ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha, Alcohol dehydrogenase 6, ADH6, ADH2, ADH4, All-trans-retinol dehydrogenase [NAD(+)] ADH4, Alcohol dehydrogenase 2, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH2, ADH1B, All-trans-retinol dehydrogenase [NAD(+)] ADH1B, Alcohol dehydrogenase 1B, Alcohol dehydrogenase subunit beta

3 Images
Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533)
  • FuncS

Supplier Data

Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533)

Sample : Bovine Liver extraction (36µg protein)

Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533)
  • FuncS

Lab

Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533)

Colorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted.

Schematic Diagram - Alcohol Dehydrogenase Assay Kit (AB102533)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Alcohol Dehydrogenase Assay Kit (AB102533)

Representative image of Alcohol Dehydrogenase Assay Kit ab102533

Components shown from left to right :

- NADH Standard I

- Substrate XII

- Developer Mix S

- ADH Positive Control

- Assay Buffer 34

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Sensitivity

= 0.01 mU/well

Assay time

40m

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB155893

ALDH Activity Assay Kit (Colorimetric)

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

  • 5
  • 1
Assay Kits

AB65343

Ethanol Assay Kit

Functional Studies - Ethanol Assay Kit (AB65343)

  • 0
  • 0
Assay Kits

AB112113

Aldehyde Assay Kit (Colorimetric)

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

  • 5
  • 1
Assay Kits

AB83464

Catalase Activity Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Catalase Activity Assay Kit (Colorimetric/Fluorometric) (AB83464)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol dehydrogenase (ADH) activity in a variety of samples.

Alcohol dehydrogenase assay principle
In the assay, alcohol dehydrogenase acts on ethanol, a chemically coupled reaction with a probe results in a colorimetric (450 nm) readout. This assay detects ADH activity as low as 0.01 mU in samples.

Alcohol dehydrogenase assay protocol summary
- add samples and standards to wells
- add reaction mix
- incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again

Other notes
This product was previously called K787 Biovision Alcohol Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102533-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Substrate XII", "number":"AB102533-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix S", "number":"AB102533-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 34", "number":"AB102533-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ADH Positive Control", "number":"AB102533-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB102533-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.
Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.
style
left-column

Product protocols

style
left-column

Target data

Alcohol dehydrogenase. Exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
See full target information ADH1C

Additional targets

ADH1A,ADH6,ADH5,ADH4,ADH1B,ADH7
style
left-column

Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 194:403-410 PubMed38880794

2024

Occasional and constant exposure to dietary ethanol shortens the lifespan of worker honey bees.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Ostap-Chec,Daniel Bajorek,Weronika Antoł,Daniel Stec,Krzysztof Miler

Antioxidants (Basel, Switzerland) 13: PubMed38790646

2024

Liquid Chromatography/Tandem Mass Spectrometry Analysis of Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Jin Yang,Min Jung Kim,Ju-Hye Yang,Ji Woong Heo,Hun Hwan Kim,Woo H Kim,Gon Sup Kim,Hu-Jang Lee,Young Woo Kim,Kwang Youn Kim,Kwang Il Park

Journal of microbiology and biotechnology 34:838-845 PubMed38247212

2024

Oral Administration of Alcohol-Tolerant Lactic Acid Bacteria Alleviates Blood Alcohol Concentration and Ethanol-Induced Liver Damage in Rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Misun Yun,Hee Eun Jo,Namhee Kim,Hyo Kyeong Park,Young Seo Jang,Ga Hee Choi,Ha Eun Jo,Jeong Hyun Seo,Ji Ye Mok,Sang Min Park,Hak-Jong Choi

Antioxidants (Basel, Switzerland) 12: PubMed37891964

2023

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin Ahn

Heliyon 8:e10737 PubMed36193527

2022

Turmeric extract ( L.) regulates hepatic toxicity in a single ethanol binge rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Hwa-Young Lee,Geum-Hwa Lee,The-Hiep Hoang,Seung Wook Kim,Choon Gil Kang,Jae Hyeok Jo,Myoung Ja Chung,Kyunghyun Min,Han-Jung Chae

ACS nano 16:14443-14455 PubMed36037396

2022

A Preparative Mass Spectrometer to Deposit Intact Large Native Protein Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Fremdling,Tim K Esser,Bodhisattwa Saha,Alexander A Makarov,Kyle L Fort,Maria Reinhardt-Szyba,Joseph Gault,Stephan Rauschenbach

iScience 25:103829 PubMed35198889

2022

A positive feed-forward loop between and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Yao Hsueh,Qiang Huang,Hongli Gong,Yujie Shen,Ji Sun,Hui-Ching Lau,Duo Zhang,Di Tang,Chunping Wu,Yang Guo,Huiying Huang,Pengyu Cao,Lei Tao,Ming Zhang,Liang Zhou

Nutrients 13: PubMed34959956

2021

Combined Water Extracts from Oxidation-Treated Leaves and Branches of Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyun Je,Miyoung Song,Ji Hyeong Baek,Jae Soon Kang,Hye Jin Chung,Kwonsu Lee,Sang Won Park,Hyun Joon Kim

Beneficial microbes 12:583-599 PubMed34550056

2021

ATCC 6475 metabolites upregulate the serotonin transporter in the intestinal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

M Engevik,W Ruan,C Visuthranukul,Z Shi,K A Engevik,A C Engevik,R Fultz,D A Schady,J K Spinler,J Versalovic

Communications biology 4:933 PubMed34413463

2021

Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Yao,Qingyuan He,Shangying Bai,Hang Zhao,Jun Yang,Dehua Cui,Yan Yu,Xuechao Fei,Yufei Mei,Ye Cheng,Shi Yan,Nayan Huang,Yalan Di,Xianjie Cai,Rui Wang,Yajuan Gao,Fangxiao Cheng,Shengjie Zhao,Xu Yang,Xiang Cai,Hongbin Han,Jihui Lyu,Zhiqian Tong
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com