Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples.
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- Antioxidants (Basel, Switzerland) 12:2023Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin AhnPubMed 37891964
- Heliyon 8:e107372022Turmeric extract ( L.) regulates hepatic toxicity in a single ethanol binge rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwa-Young Lee,Geum-Hwa Lee,The-Hiep Hoang,Seung Wook Kim,Choon Gil Kang,Jae Hyeok Jo,Myoung Ja Chung,Kyunghyun Min,Han-Jung ChaePubMed 36193527
- ACS nano 16:14443-144552022A Preparative Mass Spectrometer to Deposit Intact Large Native Protein Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Fremdling,Tim K Esser,Bodhisattwa Saha,Alexander A Makarov,Kyle L Fort,Maria Reinhardt-Szyba,Joseph Gault,Stephan RauschenbachPubMed 36037396
- iScience 25:1038292022A positive feed-forward loop between and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Yao Hsueh,Qiang Huang,Hongli Gong,Yujie Shen,Ji Sun,Hui-Ching Lau,Duo Zhang,Di Tang,Chunping Wu,Yang Guo,Huiying Huang,Pengyu Cao,Lei Tao,Ming Zhang,Liang ZhouPubMed 35198889
- Nutrients 13:2021Combined Water Extracts from Oxidation-Treated Leaves and Branches of Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihyun Je,Miyoung Song,Ji Hyeong Baek,Jae Soon Kang,Hye Jin Chung,Kwonsu Lee,Sang Won Park,Hyun Joon KimPubMed 34959956
- Beneficial microbes 12:583-5992021ATCC 6475 metabolites upregulate the serotonin transporter in the intestinal epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Engevik,W Ruan,C Visuthranukul,Z Shi,K A Engevik,A C Engevik,R Fultz,D A Schady,J K Spinler,J VersalovicPubMed 34550056
- Communications biology 4:9332021Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Yao,Qingyuan He,Shangying Bai,Hang Zhao,Jun Yang,Dehua Cui,Yan Yu,Xuechao Fei,Yufei Mei,Ye Cheng,Shi Yan,Nayan Huang,Yalan Di,Xianjie Cai,Rui Wang,Yajuan Gao,Fangxiao Cheng,Shengjie Zhao,Xu Yang,Xiang Cai,Hongbin Han,Jihui Lyu,Zhiqian TongPubMed 34413463
- Frontiers in oncology 11:6445922021Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E PongraczPubMed 34178631
- Antioxidants (Basel, Switzerland) 10:2021Electrolyzed Hydrogen Water Protects against Ethanol-Induced Cytotoxicity by Regulating Aldehyde Metabolism-Associated Enzymes in the Hepatic Cell Line HepG2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Yano,Jinyun Wang,Shigeru Kabayama,Taichi HaraPubMed 34069398
- RSC medicinal chemistry 11:1402-14122020A benzoxazole derivative as an inhibitor of anaerobic choline metabolism by human gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoustafa T Gabr,David Machalz,Szymon Pach,Gerhard WolberPubMed 34095847
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Enzyme activity
- Assay time
40m
- Sensitivity
= 0.01 mU/well
Targets
ADH1A, ADH6, ADH5, ADH4, ADH1B, ADH7
Alternative names
Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma, ADH1C, ADH3
What's included?
Recommended products
Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples.
Alternative names
Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma, ADH1C, ADH3
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Enzyme activity
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
= 0.01 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Visit our FAQs page for tips and troubleshooting.
Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again
This product is manufactured by BioVision, an Abcam company and was previously called K787 Alcohol Dehydrogenase Activity Colorimetric Assay Kit. K787-100 is the same size as the 100 test size of ab102533.
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.
- Biological function summary
Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.
- Pathways
Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.
- Associated diseases and disorders
The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.
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2 product images
Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533)
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533)
Sample: Bovine Liver extraction (36µg protein)
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