Skip to main content

Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples.


Images

Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533), expandable thumbnail
  • Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533), expandable thumbnail

Publications

  • Antioxidants (Basel, Switzerland) 12:2023
    Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Hyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin Ahn
    PubMed 37891964
  • Heliyon 8:e107372022
    Turmeric extract ( L.) regulates hepatic toxicity in a single ethanol binge rat model.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Hwa-Young Lee,Geum-Hwa Lee,The-Hiep Hoang,Seung Wook Kim,Choon Gil Kang,Jae Hyeok Jo,Myoung Ja Chung,Kyunghyun Min,Han-Jung Chae
    PubMed 36193527

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Assay time

40m

Sensitivity

= 0.01 mU/well

Alternative names

What's included?

100 Test
Components
ADH Positive Control
1 x 1 Vial
Assay Buffer XXXIV
1 x 25 mL
Developer VIII
1 x 1 Vial
NADH Standard I
1 x 1 Vial
Substrate XII
1 x 1 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Assay time

40m

Assay Platform

Microplate reader

Sensitivity

= 0.01 mU/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Visit our FAQs page for tips and troubleshooting.

Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again

This product is manufactured by BioVision, an Abcam company and was previously called K787 Alcohol Dehydrogenase Activity Colorimetric Assay Kit. K787-100 is the same size as the 100 test size of ab102533.

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.

Supplementary info

Activity summary

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.

Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

Associated diseases and disorders

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533), expandable thumbnail

    Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533)

    Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533), expandable thumbnail

    Functional Studies - Alcohol Dehydrogenase Assay Kit (ab102533)

    Sample: Bovine Liver extraction (36µg protein)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com