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AB272518

Alcohol dehydrogenase Assay Kit

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Alcohol Dehydrogenase Assay Kit (ab272518) is a non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm.

View Alternative Names

ADH3, ADH1C, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma

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Enzyme activity assay - Alcohol dehydrogenase Assay Kit (AB272518)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Alcohol dehydrogenase Assay Kit (AB272518)

ADH titration curves.

Typical titration curves – data provided for demonstration purposes only.

Example of Alcohol Dehydrogenase titration curves.

OD565 readings (left) and calculated activity (right).

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Quantitative

Range

0.4 - 80 mU/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alcohol Dehydrogenase Assay Kit (ab272518) is a non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Fast and Sensitive: Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.

Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab272518-100Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB272518-CMP02", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"MTT Solution", "number":"AB272518-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Substrate (10% Ethanol)", "number":"AB272518-CMP01", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Calibrator", "number":"AB272518-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"Diaphorase", "number":"AB272518-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"NAD Solution", "number":"AB272518-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.
Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.
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Product protocols

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Target data

Alcohol dehydrogenase. Exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
See full target information ADH1C

Additional targets

ADH1A,ADH6,ADH5,ADH4,ADH1B,ADH7
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