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AB112113

Aldehyde Assay Kit (Colorimetric)

5

(1 Review)

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(3 Publications)

Aldehyde Assay Kit (Colorimetric) ab112113 is a a sensitive, one-step colorimetric assay to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM).
6 Images
Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
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Lab

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
  • FuncS

Lab

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Aldehyde measured in biological fluids showing quantity (uM) per mL of tested sample. Values calculated based on the 550 nm Standard Curve. Samples diluted 1-10 fold.

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
  • FuncS

Lab

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Aldehyde measured in cell lysates showing quantity (uM) per 1 mln cells. Values calculated based on the 550 nm Standard Curve.

Samples with the concentration of 1.26e8 cells/mL (HeLa) and 1e8 cells/mL (HepG2) were used. Samples were diluted 3 fold.

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
  • FuncS

Lab

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
  • FuncS

Lab

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Aldehyde measured in cell culture supernatants showing quantity (uM) per mL of tested sample. Values calculated based on the 405 nm Standard Curve. Samples diluted 2 fold.

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)
  • FuncS

Supplier Data

Functional Studies - Aldehyde Assay Kit (Colorimetric) (AB112113)

Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Plasma, Cell culture extracts, Suspension cells, Adherent cells

Results type

Quantitative

Assay Platform

Microplate reader

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Product details

Aldehyde Assay Kit (Colorimetric) ab112113 is a a sensitive, one-step colorimetric assay to detect as little as 1 nanomole of aldehyde in a 100 μL assay volume (10 μM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step.

The aldehyde assay protocol uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.

The signal is read with an absorbance microplate reader at 405 or 550 nm.

Aldehyde assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader

Previously called Aldehyde Quantification Assay Kit (Colorimetric).

This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.

ab112113 should be stored desiccated.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldehydes play an important role in various biological processes. Also known as aliphatic aldehydes or aromatic aldehydes when describing specific types aldehydes can be seen throughout different environments in the human body. These small organic compounds possess a molecular mass that varies depending on the specific type of aldehyde but they generally consist of a carbonyl center with a hydrogen atom single-bonded to the carbon atom making them relatively lightweight molecules. Aldehydes are highly reactive and widely distributed in several tissues and organs such as the liver where they are heavily expressed due to the organ's metabolic functions.
Biological function summary

Aldehydes do several critical tasks in the body. They are involved in metabolic processes such as energy production and detoxification. Aldehydes can also act as signaling molecules to influence various pathways. Aldehyde dehydrogenase enzymes often act in conjunction with aldehydes transforming them into less reactive carboxylic acids. This metabolic action highlights their role as part of larger multistep enzymatic pathways demonstrating their involvement in cellular metabolism and chemical detoxification processes.

Pathways

Aldehydes participate in critical metabolic pathways including gluconeogenesis and ethanol metabolism. In the ethanol metabolism pathway the enzyme alcohol dehydrogenase initially converts ethanol into acetaldehyde which aldehyde dehydrogenase then oxidizes to acetic acid. This pathway plays a role in both energy production and cellular detoxification. Aldehydes also interact with proteins such as cytochrome P450 enzymes which are key components of drug metabolism pathways assisting in the processing of endogenous and exogenous compounds.

Aldehydes have been associated with conditions like alcohol-related liver disease and Alzheimer's disease. Acetaldehyde a type of aldehyde is implicated in the toxicity of alcohol consumption leading to liver damage when accumulated excessively. Elevated levels of aldehydes have also been detected in neurodegenerative conditions; they can contribute to oxidative stress and neuronal damage. Proteins such as amyloid-beta in Alzheimer's disease are influenced by aldehydes which can exacerbate pathological states. Understanding aldehyde function provides insight into both normal physiology and these disease mechanisms.
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:103829 PubMed35198889

2022

A positive feed-forward loop between and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Yao Hsueh,Qiang Huang,Hongli Gong,Yujie Shen,Ji Sun,Hui-Ching Lau,Duo Zhang,Di Tang,Chunping Wu,Yang Guo,Huiying Huang,Pengyu Cao,Lei Tao,Ming Zhang,Liang Zhou

Foods (Basel, Switzerland) 10: PubMed34681429

2021

Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Root Extract in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Naila Boby,Eon-Bee Lee,Muhammad Aleem Abbas,Na-Hye Park,Sam-Pin Lee,Md Sekendar Ali,Seung-Jin Lee,Seung-Chun Park

Nature communications 8:1257 PubMed29097652

2017

Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Li,Jennifer M Brazill,Sha Liu,Christofer Bello,Yi Zhu,Marie Morimoto,Lauren Cascio,Rini Pauly,Zoraida Diaz-Perez,May Christine V Malicdan,Hongbo Wang,Luigi Boccuto,Charles E Schwartz,William A Gahl,Cornelius F Boerkoel,R Grace Zhai
View all publications
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