Skip to main content

Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923) provides a sensitive mix-and-read method to detect aldehyde in a variety of samples such as cell lysates or biological fluids.

Be the first to review this product! Submit a review

Images

Functional Studies - Aldehyde Assay Kit II (Colorimetric - Blue) (AB219923), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

200 Test
Components
Aldehyde Standard
1 x 1 Vial
Assay Buffer
1 x 25 mL
Blue Dye
2 x 1 Unit
Blue Enhancer
1 x 10 mL

Recommended products

Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923) provides a sensitive mix-and-read method to detect aldehyde in a variety of samples such as cell lysates or biological fluids.

Key facts

Detection method

Colorimetric

Sample types

Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923) provides a sensitive mix-and-read method to detect aldehyde in a variety of samples such as cell lysates or biological fluids. This assay used a proprietary sensor that upon reacting with an aldehyde, generates a chromogenic product that can be detected by absorbance on a microplate reader at OD620-660 nm. This assay can detect as little as 3 μM aldehyde (0.3 nmol/100 μL assay volume).

The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation step.

If your plate reader does not have the appropriate filter to detect the absorbance change, we recommend Aldehyde Quantification Assay Kit (Colorimetric) (Aldehyde Assay Kit (Colorimetric) ab112113), which can be read at OD405 or OD550 nm.

Very reactive aldehydes, namely 4-hydroxyalkenals, were first shown to be formed in autoxidizing chemical systems. It was subsequently shown that 4-hydroxyalkenals, particularly 4-hydroxynonenal, were formed in substantial amounts under biological conditions, i.e. during the peroxidation of lipids of liver microsomes incubated in the NADPH-Fe system. Many other aldehydes were also identified in peroxidizing liver microsomes orhepatocytes, e.g., alkanals, alk-2-enals, and 4-hydroxyalkenals.

Rapid and accurate measurement of aldehydes is an important task for biological and chemical research, as well as food industry and environmental pollution surveillance.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aldehydes play an important role in various biological processes. Also known as aliphatic aldehydes or aromatic aldehydes when describing specific types aldehydes can be seen throughout different environments in the human body. These small organic compounds possess a molecular mass that varies depending on the specific type of aldehyde but they generally consist of a carbonyl center with a hydrogen atom single-bonded to the carbon atom making them relatively lightweight molecules. Aldehydes are highly reactive and widely distributed in several tissues and organs such as the liver where they are heavily expressed due to the organ's metabolic functions.

Biological function summary

Aldehydes do several critical tasks in the body. They are involved in metabolic processes such as energy production and detoxification. Aldehydes can also act as signaling molecules to influence various pathways. Aldehyde dehydrogenase enzymes often act in conjunction with aldehydes transforming them into less reactive carboxylic acids. This metabolic action highlights their role as part of larger multistep enzymatic pathways demonstrating their involvement in cellular metabolism and chemical detoxification processes.

Pathways

Aldehydes participate in critical metabolic pathways including gluconeogenesis and ethanol metabolism. In the ethanol metabolism pathway the enzyme alcohol dehydrogenase initially converts ethanol into acetaldehyde which aldehyde dehydrogenase then oxidizes to acetic acid. This pathway plays a role in both energy production and cellular detoxification. Aldehydes also interact with proteins such as cytochrome P450 enzymes which are key components of drug metabolism pathways assisting in the processing of endogenous and exogenous compounds.

Associated diseases and disorders

Aldehydes have been associated with conditions like alcohol-related liver disease and Alzheimer's disease. Acetaldehyde a type of aldehyde is implicated in the toxicity of alcohol consumption leading to liver damage when accumulated excessively. Elevated levels of aldehydes have also been detected in neurodegenerative conditions; they can contribute to oxidative stress and neuronal damage. Proteins such as amyloid-beta in Alzheimer's disease are influenced by aldehydes which can exacerbate pathological states. Understanding aldehyde function provides insight into both normal physiology and these disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923), expandable thumbnail

    Functional Studies - Aldehyde Assay Kit II (Colorimetric - Blue) (ab219923)

    Aldehyde Quantification Assay Kit II (Colorimetric) (ab219923). Typical aldehyde standard calibration curve. Aldehyde dose response was measured in a white wall/clear bottom 96-well plate using a SpectraMax microplate reader. As low as 3 μM aldehyde can be detected with 30 minutes incubation (n=3).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com