ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Antioxidants (Basel, Switzerland) 12:2023Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin AhnPubMed 37891964
- Cell death and differentiation 30:1247-12592023The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianing Tang,Qian Yang,Chao Mao,Desheng Xiao,Shuang Liu,Liang Xiao,Ledu Zhou,Gaosong Wu,Yongguang TaoPubMed 36813921
- Heliyon 8:e107372022Turmeric extract ( L.) regulates hepatic toxicity in a single ethanol binge rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwa-Young Lee,Geum-Hwa Lee,The-Hiep Hoang,Seung Wook Kim,Choon Gil Kang,Jae Hyeok Jo,Myoung Ja Chung,Kyunghyun Min,Han-Jung ChaePubMed 36193527
- Nutrients 13:2021Combined Water Extracts from Oxidation-Treated Leaves and Branches of Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihyun Je,Miyoung Song,Ji Hyeong Baek,Jae Soon Kang,Hye Jin Chung,Kwonsu Lee,Sang Won Park,Hyun Joon KimPubMed 34959956
- Frontiers in oncology 11:6445922021Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E PongraczPubMed 34178631
- Antioxidants (Basel, Switzerland) 10:2021Electrolyzed Hydrogen Water Protects against Ethanol-Induced Cytotoxicity by Regulating Aldehyde Metabolism-Associated Enzymes in the Hepatic Cell Line HepG2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Yano,Jinyun Wang,Shigeru Kabayama,Taichi HaraPubMed 34069398
- Cancer letters 503:163-1732021In vivo selection of highly metastatic human ovarian cancer sublines reveals role for AMIGO2 in intra-peritoneal metastatic regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueying Liu,Jing Yang,Zonggao Shi,Xuejuan Tan,Norman Jin,Catlin O'Brien,Connor Ott,Anna Grisoli,Eric Lee,Kelly Volk,Meghan Conroy,Emily Franz,Annamarie Bryant,Leigh Campbell,Brian Crowley,Stephen Grisoli,Aris T Alexandrou,Chunyan Li,Elizabeth I Harper,Marwa Asem,Jeff Johnson,Annemarie Leonard,Katie Santanello,Ashley Klein,Qingfei Wang,Siyuan Zhang,Tyvette S Hilliard,M Sharon StackPubMed 33524500
- Frontiers in molecular biosciences 7:1162020Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Furuhashi,Ryuga Ishii,Haruka Onishi,Shigenori OtaPubMed 32695794
- Journal of clinical medicine 8:2019B4GALT1 Is a New Candidate to Maintain the Stemness of Lung Cancer Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia De Vitis et. al.PubMed 31717588
- Cancer cell international 19:722019LncRNA BCAR4, targeting to miR-665/STAT3 signaling, maintains cancer stem cells stemness and promotes tumorigenicity in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShurui Ouyang et. al.PubMed 30962766
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue Culture Media
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
30m
- Sensitivity
< 0.5 mU/well
Associated Products
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Target data
Function
Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:9133646, PubMed:22633490, PubMed:25047030, PubMed:18035827, PubMed:9662422, PubMed:18182499). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:22633490).
Targets
ALDH1B1, ALDH8A1, ALDH3B1, ALDH3B2
Alternative names
Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase, ALDH10, FALDH, ALDH3A2
What's included?
Recommended products
ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase, ALDH10, FALDH, ALDH3A2
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue Culture Media
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
30m
- Assay Platform
Microplate reader
- Sensitivity
< 0.5 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity in a variety of samples.
ALDH assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze with a microplate reader at 5 min and 20-60 min
This product is manufactured by BioVision, an Abcam company and was previously called K731 Aldehyde Dehydrogenase Activity Colorimetric Assay Kit. K731-100 is the same size as the 100 test size of ab155893.
The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldehyde dehydrogenase often called ALDH is an enzyme family that plays an important mechanical role in cellular metabolism by oxidizing aldehydes into carboxylic acids. The molecular mass of ALDH enzymes varies but they are generally around 50-56 kDa. ALDH is mainly expressed in the liver as well as in other tissues such as the kidney and the stomach. The enzyme is known for its role in detoxifying alcohol-derived acetaldehyde a toxic compound therefore protecting the cells from oxidative stress.
- Biological function summary
Aldehyde dehydrogenase enzymes significantly contribute to metabolic processes by helping the conversion of aldehydes. ALDH is part of a complex network of enzymes involved in cellular homeostasis. This family of enzymes influences the regulation of cell proliferation and differentiation due to its metabolic function. The ability of ALDH to maintain low levels of reactive aldehydes is important for many cellular activities linking these enzymes to protective and regulatory roles within the cell.
- Pathways
Aldehyde dehydrogenase enzymes are integral to alcohol metabolism and the detoxification pathway in humans. They play a role in the oxidizing step of ethanol metabolism to prevent the accumulation of acetaldehyde. Additionally ALDH enzymes interact with related proteins such as alcohol dehydrogenase (ADH) which converts ethanol into acetaldehyde. This interaction in the metabolic pathway keeps toxic levels of aldehyde in check and supports overall cellular metabolism and detoxification pathways.
- Associated diseases and disorders
Altered expression or function of aldehyde dehydrogenase has links to alcoholism and certain cancers. Insufficient ALDH activity may lead to alcohol intolerance or liver disease due to buildup of toxic acetaldehyde. In cancer ALDH expression serves as a marker for cancer stem cells in various types of tumors. In both cases ALDH interactions with proteins such as acetaldehyde dehydrogenase and related metabolic enzymes play a role in the pathogenesis or progression of these conditions.
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3 product images
Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)
Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)
ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.
Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)
ALDH activity in Positive Control.
Typical OD of the diffrent positive contols mesuared over 1 hour.
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