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ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893), expandable thumbnail
  • Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893), expandable thumbnail
  • Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue Culture Media

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

30m

Sensitivity

< 0.5 mU/well

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:9133646, PubMed:22633490, PubMed:25047030, PubMed:18035827, PubMed:9662422, PubMed:18182499). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:22633490).

Targets

ALDH1B1, ALDH8A1, ALDH3B1, ALDH3B2

Alternative names

What's included?

100 Test
Components
ALDH Positive Control
1 x 1 Vial
Acetaldehyde
1 x 0.5 mL
Assay Buffer XXVIII
1 x 25 mL
Developer Solution III
1 x 1 Vial
NADH Standard I
1 x 1 Vial

Recommended products

ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue Culture Media

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

30m

Assay Platform

Microplate reader

Sensitivity

< 0.5 mU/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity in a variety of samples.

ALDH assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze with a microplate reader at 5 min and 20-60 min

This product is manufactured by BioVision, an Abcam company and was previously called K731 Aldehyde Dehydrogenase Activity Colorimetric Assay Kit. K731-100 is the same size as the 100 test size of ab155893.

The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aldehyde dehydrogenase often called ALDH is an enzyme family that plays an important mechanical role in cellular metabolism by oxidizing aldehydes into carboxylic acids. The molecular mass of ALDH enzymes varies but they are generally around 50-56 kDa. ALDH is mainly expressed in the liver as well as in other tissues such as the kidney and the stomach. The enzyme is known for its role in detoxifying alcohol-derived acetaldehyde a toxic compound therefore protecting the cells from oxidative stress.

Biological function summary

Aldehyde dehydrogenase enzymes significantly contribute to metabolic processes by helping the conversion of aldehydes. ALDH is part of a complex network of enzymes involved in cellular homeostasis. This family of enzymes influences the regulation of cell proliferation and differentiation due to its metabolic function. The ability of ALDH to maintain low levels of reactive aldehydes is important for many cellular activities linking these enzymes to protective and regulatory roles within the cell.

Pathways

Aldehyde dehydrogenase enzymes are integral to alcohol metabolism and the detoxification pathway in humans. They play a role in the oxidizing step of ethanol metabolism to prevent the accumulation of acetaldehyde. Additionally ALDH enzymes interact with related proteins such as alcohol dehydrogenase (ADH) which converts ethanol into acetaldehyde. This interaction in the metabolic pathway keeps toxic levels of aldehyde in check and supports overall cellular metabolism and detoxification pathways.

Associated diseases and disorders

Altered expression or function of aldehyde dehydrogenase has links to alcoholism and certain cancers. Insufficient ALDH activity may lead to alcohol intolerance or liver disease due to buildup of toxic acetaldehyde. In cancer ALDH expression serves as a marker for cancer stem cells in various types of tumors. In both cases ALDH interactions with proteins such as acetaldehyde dehydrogenase and related metabolic enzymes play a role in the pathogenesis or progression of these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893), expandable thumbnail

    Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)

    Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.

  • Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893), expandable thumbnail

    Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)

    ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

  • Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893), expandable thumbnail

    Functional Studies - ALDH Activity Assay Kit (Colorimetric) (ab155893)

    ALDH activity in Positive Control.

    Typical OD of the diffrent positive contols mesuared over 1 hour.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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