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AB155893

ALDH Activity Assay Kit (Colorimetric)

5

(1 Review)

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(18 Publications)

ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

ALDH10, FALDH, ALDH3A2, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase, ALDH5, ALDHX, ALDH1B1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase family 1 member B1, ALDH7, ALDH3B1, Aldehyde dehydrogenase family 3 member B1, Aldehyde dehydrogenase 7, Long-chain fatty aldehyde dehydrogenase, Medium-chain fatty aldehyde dehydrogenase, ALDH8, ALDH3B2, Aldehyde dehydrogenase family 3 member B2, Aldehyde dehydrogenase 8, Long-chain fatty aldehyde dehydrogenase, ALDH12, ALDH8A1, 2-aminomuconic semialdehyde dehydrogenase, Aldehyde dehydrogenase 12, Aldehyde dehydrogenase family 8 member A1

4 Images
Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)
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Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)
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Lab

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)
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Supplier Data

Functional Studies - ALDH Activity Assay Kit (Colorimetric) (AB155893)

ALDH activity in Positive Control.

Typical OD of different amounts of activity of the positive control.

Other - ALDH Activity Assay Kit (Colorimetric) (AB155893)
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Supplier Data

Other - ALDH Activity Assay Kit (Colorimetric) (AB155893)

Representative image of ALDH Activity Assay Kit (Colorimetric) ab155893

Components shown from left to right :

- NADH Standard I

- Acetaldehyde

- Developer Solution III

- ALDH Positive Control

- Assay Buffer 28

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell Lysate, Tissue Homogenate, Tissue Lysate

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

< 0.1 mU/well

Assay time

30m

Assay Platform

Microplate reader

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Complementary Products

Assay Kits

AB102533

Alcohol Dehydrogenase Assay Kit

Functional Studies - Alcohol Dehydrogenase Assay Kit (AB102533)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB115348

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

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Assay Kits

AB155894

ALDH Activity Assay Kit (Fluorometric)

Functional Studies - ALDH Activity Assay Kit (Fluorometric) (AB155894)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify ALDH enzymatic activity

ALDH assay principle
In the ALDH assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm.

ALDH assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate for 20-60 min
- Analyze with a microplate reader at 5 min and 20-60 min

Related and recommended products
For fluorometric detection, we recommend ALDH Activity Assay Kit (Fluorometric) ab155894
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 uses antibody-pulldown to specifically measure the activity of mitochondrial ALDH2.

Other notes
This product was previously called K731 Biovision Aldehyde Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155893-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB155893-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB155893-CMP04", "productcode":"AB255098" }, { "size":"1 x 25 mL", "name":"Assay Buffer 28", "number":"AB155893-CMP02", "productcode":"AB323987" }, { "size":"1 x 0.5 mL", "name":"Acetaldehyde", "number":"AB155893-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ALDH Positive Control", "number":"AB155893-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldehyde dehydrogenase often called ALDH is an enzyme family that plays an important mechanical role in cellular metabolism by oxidizing aldehydes into carboxylic acids. The molecular mass of ALDH enzymes varies but they are generally around 50-56 kDa. ALDH is mainly expressed in the liver as well as in other tissues such as the kidney and the stomach. The enzyme is known for its role in detoxifying alcohol-derived acetaldehyde a toxic compound therefore protecting the cells from oxidative stress.
Biological function summary

Aldehyde dehydrogenase enzymes significantly contribute to metabolic processes by helping the conversion of aldehydes. ALDH is part of a complex network of enzymes involved in cellular homeostasis. This family of enzymes influences the regulation of cell proliferation and differentiation due to its metabolic function. The ability of ALDH to maintain low levels of reactive aldehydes is important for many cellular activities linking these enzymes to protective and regulatory roles within the cell.

Pathways

Aldehyde dehydrogenase enzymes are integral to alcohol metabolism and the detoxification pathway in humans. They play a role in the oxidizing step of ethanol metabolism to prevent the accumulation of acetaldehyde. Additionally ALDH enzymes interact with related proteins such as alcohol dehydrogenase (ADH) which converts ethanol into acetaldehyde. This interaction in the metabolic pathway keeps toxic levels of aldehyde in check and supports overall cellular metabolism and detoxification pathways.

Altered expression or function of aldehyde dehydrogenase has links to alcoholism and certain cancers. Insufficient ALDH activity may lead to alcohol intolerance or liver disease due to buildup of toxic acetaldehyde. In cancer ALDH expression serves as a marker for cancer stem cells in various types of tumors. In both cases ALDH interactions with proteins such as acetaldehyde dehydrogenase and related metabolic enzymes play a role in the pathogenesis or progression of these conditions.
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Product protocols

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Target data

Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed : 18035827, PubMed : 18182499, PubMed : 22633490, PubMed : 25047030, PubMed : 9133646, PubMed : 9662422). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed : 22633490).
See full target information ALDH3A2

Additional targets

ALDH1B1,ALDH8A1,ALDH3B1,ALDH3B2
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39768178

2025

Co-Inhibition of tGLI1 and GP130 Using FDA-Approved Ketoconazole and Bazedoxifene Is Synergistic Against the Growth and Metastasis of HER2-Enriched and Triple-Negative Breast Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Manore,Chuling Zhuang,Mariana K Najjar,Grace L Wong,Shivani Bindal,Kounosuke Watabe,Jiayuh Lin,Hui-Wen Lo

Cell death & disease 15:568 PubMed39107297

2024

ALDH1A1 promotes immune escape of tumor cells through ZBTB7B-glycolysis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyuan Wang,Taoli Wang,Jinjin Wang,Yuexin Yang,Xi Li,Huan Chen,Jingnan Liao

Cancer letters 587:216724 PubMed38373689

2024

CD24 negativity reprograms mitochondrial metabolism to PPARα and NF-κB-driven fatty acid β-oxidation in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Divya Murthy,Debasmita Dutta,Kuldeep S Attri,Tagari Samanta,Sukjin Yang,Kwang Hwa Jung,Sarah G Latario,Vasanta Putluri,Shixia Huang,Nagireddy Putluri,Jun Hyoung Park,Benny Abraham Kaipparettu

Cancer research communications 4:571-587 PubMed38329386

2024

INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tsima Abou Kors,Linda Hofmann,Annika Betzler,Kathrina Payer,Martin Bens,Jens Truong,Adrian von Witzleben,Jaya Thomas,Johann M Kraus,Randa Kalaajieh,Diana Huber,Jasmin Ezić,Julian Benckendorff,Jens Greve,Patrick J Schuler,Christian H Ottensmeier,Hans A Kestler,Thomas K Hoffmann,Marie-Nicole Theodoraki,Cornelia Brunner,Simon Laban

Journal of microbiology and biotechnology 34:838-845 PubMed38247212

2024

Oral Administration of Alcohol-Tolerant Lactic Acid Bacteria Alleviates Blood Alcohol Concentration and Ethanol-Induced Liver Damage in Rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Misun Yun,Hee Eun Jo,Namhee Kim,Hyo Kyeong Park,Young Seo Jang,Ga Hee Choi,Ha Eun Jo,Jeong Hyun Seo,Ji Ye Mok,Sang Min Park,Hak-Jong Choi

Antioxidants (Basel, Switzerland) 12: PubMed37891964

2023

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin Ahn

Cell death and differentiation 30:1247-1259 PubMed36813921

2023

The deubiquitinating enzyme UCHL3 promotes anaplastic thyroid cancer progression and metastasis through Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianing Tang,Qian Yang,Chao Mao,Desheng Xiao,Shuang Liu,Liang Xiao,Ledu Zhou,Gaosong Wu,Yongguang Tao

Heliyon 8:e10737 PubMed36193527

2022

Turmeric extract ( L.) regulates hepatic toxicity in a single ethanol binge rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Hwa-Young Lee,Geum-Hwa Lee,The-Hiep Hoang,Seung Wook Kim,Choon Gil Kang,Jae Hyeok Jo,Myoung Ja Chung,Kyunghyun Min,Han-Jung Chae

Nutrients 13: PubMed34959956

2021

Combined Water Extracts from Oxidation-Treated Leaves and Branches of Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyun Je,Miyoung Song,Ji Hyeong Baek,Jae Soon Kang,Hye Jin Chung,Kwonsu Lee,Sang Won Park,Hyun Joon Kim

Frontiers in oncology 11:644592 PubMed34178631

2021

Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Elhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E Pongracz
View all publications
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