JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB211112

Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(0 Publication)

Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112) provides a convenient tool for sensitive detection of Aldo-Keto Reductase (AKR) activity in animal tissue and cells, as well as biological fluids such as serum.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

ALDR1, ALR, AKR1A1, Aldo-keto reductase family 1 member A1, Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Glucuronate reductase, Glucuronolactone reductase, S-nitroso-CoA reductase, ScorR

3 Images
Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)
  • FuncS

Supplier Data

Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)

Typical NADPH standard curve.

Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)
  • FuncS

Unknown

Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)

Kinetic curves showing AKR activity detection in increase amounts of positive control included in the kit (μL/assay).

Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)
  • FuncS

Supplier Data

Functional Studies - Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (AB211112)

AKR specific activity in human tissue lysates and cell line lysates. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Serum, Cell Lysate

Assay type

Enzyme activity (quantitative)

Sensitivity

= 0.001 mU/well

Assay Platform

Microplate reader

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112) provides a convenient tool for sensitive detection of Aldo-Keto Reductase (AKR) activity in animal tissue and cells, as well as biological fluids such as serum. The assay is based on the ability of AKR to reduce a general substrate and convert NADP+ to NADPH, which reacts with the AKR probe to generates color that can be detected at OD 450 nm. The intensity of the color is proportional to the activity of AKR in the sample.

This assay can detect as low as 1 uU of AKR activity in samples. It has been validated with AKR1B10, AKR1C1 and AKR1C3.

Other Notes
This product was previously called K847 Biovision EZDetect Aldo-keto Reductase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members and are present in nearly all phyla. They are mainly monomeric soluble proteins (34-37 kD) and their common is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification.

In humans, AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab211112-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADPH Standard", "number":"AB211112-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADP Detection Probe", "number":"AB211112-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB211112-CMP01", "productcode":"AB323940" }, { "size":"1 x 10 mL", "name":"AKR Substrate", "number":"AB211112-CMP04", "productcode":"" }, { "size":"1 x 10 µL", "name":"AKR Positive Control", "number":"AB211112-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR1A1 also known as aldehyde reductase or aldo-keto reductase is a member of the aldo-keto reductase family. It functions mechanically as a reductase enzyme that converts aldehydes and ketones to their corresponding alcohols via NADPH as a cofactor. The enzyme has a molecular mass of approximately 36 kDa. AKR1A1 is expressed in a wide range of tissues including liver kidney and brain indicating its broad role in metabolic processes.
Biological function summary

AKR1A1 is involved in the detoxification of aldehydes produced during metabolism and oxidative stress. This function occurs as part of a larger detoxification system. Being part of the aldo-keto reductase family it contributes to maintaining homeostasis by regulating the levels of reactive aldehydes. Through this activity it affects cellular responses to stress and the metabolism of xenobiotics.

Pathways

AKR1A1 has significant involvement in metabolic and detoxification pathways notably in glycolysis and xenobiotic metabolism. It interacts with various aldehydes formed as intermediates in these pathways. Related proteins in these pathways include AKR1B1 which also plays a role in reducing sugar-derived aldehydes emphasizing the importance of AKR1A1 in controlling toxic aldehyde levels and protecting cellular components from damage.

AKR1A1 has been linked to diabetic complications due to its role in polyol pathway where it contributes to the conversion of glucose to sorbitol. This pathway can lead to osmotic and oxidative stress when dysregulated contributing to retinal and renal dysfunctions in diabetes. Additionally AKR1A1 is connected to cancer development where it affects oxidative stress levels and tumor progression alongside proteins like AKR1C1 which are also involved in lipid and steroid metabolism further influencing disease outcomes.
style
left-column

Product protocols

style
left-column

Target data

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed : 10510318, PubMed : 30538128). Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (PubMed : 10510318, PubMed : 30538128). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes (By similarity). Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic (By similarity). Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) (PubMed : 11306097, PubMed : 18276838). Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins (PubMed : 30538128). S-nitroso-CoA reductase activity is involved in reprogramming intermediary metabolism in renal proximal tubules, notably by inhibiting protein S-nitrosylation of isoform 2 of PKM (PKM2) (By similarity). Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione (PubMed : 31649033). Displays no reductase activity towards retinoids (By similarity).
See full target information AKR1A1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB83463

Thioredoxin Reductase Assay Kit (Colorimetric)

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

  • 0
  • 0
Primary Antibodies

AB192865

Anti-AKR1B10 antibody [EPR14421]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-AKR1B10 antibody [EPR14421] (AB192865)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB118970

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

Biochemical assay - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (AB118970)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com