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AB196994

Aldolase Activity Assay Kit (Colorimetric)

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(21 Publications)

Aldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

4 Images
Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)
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Supplier Data

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

Aldolase activity in rat kidney and muscle, HeLa and 3T3 cells. Data obtained performing assay as described in assay protocol.

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)
  • FuncS

Supplier Data

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

Relative aldolase activity in lysates prepared from rat kidney (14.2 μg), rat muscle (10.64 μg), 3T3 cells (9.97 μg) and HeLa cells (11.35 μg).

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)
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Supplier Data

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

Typical NADH Standard Curve.

Schematic Diagram - Aldolase Activity Assay Kit (Colorimetric) (AB196994)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

Representative image of Aldolase Activity Assay Kit (Colorimetric) ab196994

Components shown from left to right :

- NADH Standard I

- Aldolase Substrate

- Developer Solution III

- PGK Developer

- Aldolase Positive Control

- Assay Buffer 4

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Suspension cells, Serum, Adherent cells

Assay type

Enzyme activity

Sensitivity

< 100 µU

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Aldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.

This assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.

Other Notes
This product was previously called K665 Biovision Aldolase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone.

There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.

Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab196994-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PGK Developer", "number":"AB196994-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Aldolase Positive Control", "number":"AB196994-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB196994-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB196994-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB196994-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Aldolase Substrate", "number":"AB196994-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase also known as fructose-bisphosphate aldolase is an enzyme that plays a critical role in glycolysis catalyzing the reversible cleavage of fructose 16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Aldolase is a homotetramer with a molecular mass of approximately 158 kDa. It is highly expressed in liver muscle and brain tissues. Aldolase consists of three isoforms: aldolase A B and C each predominant in different tissues contributing to tissue-specific roles within the organism.
Biological function summary

Aldolase catalyzes an important reaction in energy metabolism ensuring the continuation of glycolytic flux which is essential for ATP production. Aldolases do not form part of larger protein complexes. However they interact with other enzymes within the glycolytic pathway to facilitate a smooth metabolic flow. Moreover aldolase A plays an additional role in gluconeogenesis the pathway involved in synthesizing glucose from non-carbohydrate sources.

Pathways

Aldolase is an essential part of glycolysis and gluconeogenesis pathways. In glycolysis it partners with enzymes like phosphofructokinase and enolase to breakdown glucose for energy production. During gluconeogenesis aldolase works with enzymes including fructose-16-bisphosphatase to create glucose vital for maintaining blood sugar levels during fasting. Through these pathways aldolase ensures balance between energy-producing and energy-consuming states within the cell.

Mutations or deficiencies in aldolase particularly aldolase A have been linked to glycogen storage disease type XII which results in muscle weakness and exercise intolerance. Aldolase B deficiency is known to cause hereditary fructose intolerance leading to liver and renal complications. These conditions highlight the importance of aldolase in maintaining metabolic health with implications for proteins within the same pathways potentially affecting their functions and expression levels.
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Product protocols

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Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).
See full target information ALDOB

Additional targets

ALDOC,ALDOA
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 26:757-766 PubMed40156391

2025

The Synergistic and Anticancer Potential of Withania Somnifera (Ashwagandha) Ethanol Extract as an Adjuvant with Doxorubicin in MCF7 Breast Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Emad M Elzayat,Ghada E Elsamahy,Ghada H Mansour,Ahmed A El-Sherif,Nourhan Hassan

Scientific reports 14:15388 PubMed38965296

2024

Proteomic analysis of Rana sylvatica reveals differentially expressed proteins in liver in response to anoxia, dehydration or freezing stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxi Li,Zoran Minic,Nico Hüttmann,Abdullah Khraibah,Kenneth B Storey,Maxim V Berezovski

Journal of lipid research 65:100525 PubMed38417553

2024

Mining cholesterol genes from thousands of mouse livers identifies aldolase C as a regulator of cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

James A Votava,Steven V John,Zhonggang Li,Shuyang Chen,Jing Fan,Brian W Parks

The Journal of biological chemistry 299:105407 PubMed38152849

2023

Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Wu,Betsy T Kren,Andrew N Lane,Teresa A Cassel,Richard M Higashi,Teresa W M Fan,George S Scaria,Laurie L Shekels,Mark A Klein,Jeffrey H Albrecht

International journal of molecular sciences 24: PubMed38003258

2023

Activation of Purine Biosynthesis Suppresses the Sensitivity of Mutant to Antibiotics.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana A Seregina,Irina Yu Petrushanko,Pavel I Zaripov,Rustem S Shakulov,Svetlana A Sklyarova,Vladimir A Mitkevich,Alexander A Makarov,Alexander S Mironov

Biomedicines 11: PubMed37189694

2023

Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Zoran Minic,Yingxi Li,Nico Hüttmann,Gurcharan K Uppal,Rochelle D'Mello,Maxim V Berezovski

The Journal of biological chemistry 299:102960 PubMed36736426

2023

The deacylase sirtuin 5 reduces malonylation in nonmitochondrial metabolic pathways in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Judy Baek,Kelli Sas,Chenchen He,Viji Nair,William Giblin,Ayaka Inoki,Hongyu Zhang,Yang Yingbao,Jeffrey Hodgin,Robert G Nelson,Frank C Brosius,Matthias Kretzler,Paul M Stemmer,David B Lombard,Subramaniam Pennathur

iScience 26:106080 PubMed36824285

2023

Global profiling of AMG510 modified proteins identified tumor suppressor KEAP1 as an off-target.

Applications

Unspecified application

Species

Unspecified reactive species

Yini Wang,Bowen Zhong,Caixia Xu,Dongdong Zhan,Songhao Zhao,Hongxing Wu,Mingwei Liu,Xiaoling Lan,Danni Cai,Qian Ding,Biao Zheng,Jiong Lan,Qiang Lv,Yi Wang,Jun Qin

Nature communications 13:6754 PubMed36376280

2022

Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Haythorne,Matthew Lloyd,John Walsby-Tickle,Andrei I Tarasov,Jonas Sandbrink,Idoia Portillo,Raul Terron Exposito,Gregor Sachse,Malgorzata Cyranka,Maria Rohm,Patrik Rorsman,James McCullagh,Frances M Ashcroft

Nature methods 19:854-864 PubMed35761067

2022

Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wan,Nian Wang,Siqin Yu,Hanqing Zhang,Shuo Tang,Dexiang Wang,Wenjie Lu,Huanhuan Li,Daniel G Delafield,Ying Kong,Xinmiao Wang,Chang Shao,Langlang Lv,Guangji Wang,Renxiang Tan,Nanxi Wang,Haiping Hao,Hui Ye
View all publications
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