Aldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 24:2023Activation of Purine Biosynthesis Suppresses the Sensitivity of Mutant to Antibiotics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatiana A Seregina,Irina Yu Petrushanko,Pavel I Zaripov,Rustem S Shakulov,Svetlana A Sklyarova,Vladimir A Mitkevich,Alexander A Makarov,Alexander S MironovPubMed 38003258
- Biomedicines 11:2023Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoran Minic,Yingxi Li,Nico Hüttmann,Gurcharan K Uppal,Rochelle D'Mello,Maxim V BerezovskiPubMed 37189694
- The Journal of biological chemistry 299:1029602023The deacylase sirtuin 5 reduces malonylation in nonmitochondrial metabolic pathways in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJudy Baek,Kelli Sas,Chenchen He,Viji Nair,William Giblin,Ayaka Inoki,Hongyu Zhang,Yang Yingbao,Jeffrey Hodgin,Robert G Nelson,Frank C Brosius,Matthias Kretzler,Paul M Stemmer,David B Lombard,Subramaniam PennathurPubMed 36736426
- iScience 26:1060802023Global profiling of AMG510 modified proteins identified tumor suppressor KEAP1 as an off-target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYini Wang,Bowen Zhong,Caixia Xu,Dongdong Zhan,Songhao Zhao,Hongxing Wu,Mingwei Liu,Xiaoling Lan,Danni Cai,Qian Ding,Biao Zheng,Jiong Lan,Qiang Lv,Yi Wang,Jun QinPubMed 36824285
- Nature communications 13:67542022Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Haythorne,Matthew Lloyd,John Walsby-Tickle,Andrei I Tarasov,Jonas Sandbrink,Idoia Portillo,Raul Terron Exposito,Gregor Sachse,Malgorzata Cyranka,Maria Rohm,Patrik Rorsman,James McCullagh,Frances M AshcroftPubMed 36376280
- Nature methods 19:854-8642022Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Wan,Nian Wang,Siqin Yu,Hanqing Zhang,Shuo Tang,Dexiang Wang,Wenjie Lu,Huanhuan Li,Daniel G Delafield,Ying Kong,Xinmiao Wang,Chang Shao,Langlang Lv,Guangji Wang,Renxiang Tan,Nanxi Wang,Haiping Hao,Hui YePubMed 35761067
- Animals : an open access journal from MDPI 12:2022Myostatin Knockout Limits Exercise-Induced Reduction in Bovine Erythrocyte Oxidative Stress by Enhancing the Efficiency of the Pentose Phosphate Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhu,Chunling Bai,Xueqiao Wang,Zhuying Wei,Mingjuan Gu,Xinyu Zhou,Guanghua Su,Xuefei Liu,Lei Yang,Guangpeng LiPubMed 35405915
- Frontiers in veterinary science 9:8565362022Reducing the Glucose Level in Pre-treatment Solution Improves Post-thaw Boar Sperm Quality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhendong Zhu,Weijing Zhang,Rongnan Li,Wenxian ZengPubMed 35433908
- International journal of environmental research and public health 19:2022Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJèssica B Bonet,Casimiro Javierre,João Tiago Guimarães,Sandra Martins,David Rizo-Roca,Jorge Beleza,Ginés Viscor,Teresa Pagès,José Magalhães,Joan R TorrellaPubMed 35010758
- Nature communications 12:13992021Staphylococcus aureus induces an itaconate-dominated immunometabolic response that drives biofilm formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKira L Tomlinson,Tania Wong Fok Lung,Felix Dach,Medini K Annavajhala,Stanislaw J Gabryszewski,Ryan A Groves,Marija Drikic,Nancy J Francoeur,Shwetha H Sridhar,Melissa L Smith,Sara Khanal,Clemente J Britto,Robert Sebra,Ian Lewis,Anne-Catrin Uhlemann,Barbara C Kahl,Alice S Prince,Sebastián A RiquelmePubMed 33658521
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
Enzyme activity
- Reactive species
Mammals
- Sensitivity
< 100 µU
Targets
ALDOC, ALDOA
Alternative names
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDB, ALDOB
What's included?
Recommended products
Aldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDB, ALDOB
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
Enzyme activity
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
< 100 µU
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Aldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
This assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.
This product is manufactured by BioVision, an Abcam company and was previously called K665 Aldolase Activity Colorimetric Assay Kit. K665-100 is the same size as the 100 test size of ab196994.
Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone.
There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.
Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldolase also known as fructose-bisphosphate aldolase is an enzyme that plays a critical role in glycolysis catalyzing the reversible cleavage of fructose 16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Aldolase is a homotetramer with a molecular mass of approximately 158 kDa. It is highly expressed in liver muscle and brain tissues. Aldolase consists of three isoforms: aldolase A B and C each predominant in different tissues contributing to tissue-specific roles within the organism.
- Biological function summary
Aldolase catalyzes an important reaction in energy metabolism ensuring the continuation of glycolytic flux which is essential for ATP production. Aldolases do not form part of larger protein complexes. However they interact with other enzymes within the glycolytic pathway to facilitate a smooth metabolic flow. Moreover aldolase A plays an additional role in gluconeogenesis the pathway involved in synthesizing glucose from non-carbohydrate sources.
- Pathways
Aldolase is an essential part of glycolysis and gluconeogenesis pathways. In glycolysis it partners with enzymes like phosphofructokinase and enolase to breakdown glucose for energy production. During gluconeogenesis aldolase works with enzymes including fructose-16-bisphosphatase to create glucose vital for maintaining blood sugar levels during fasting. Through these pathways aldolase ensures balance between energy-producing and energy-consuming states within the cell.
- Associated diseases and disorders
Mutations or deficiencies in aldolase particularly aldolase A have been linked to glycogen storage disease type XII which results in muscle weakness and exercise intolerance. Aldolase B deficiency is known to cause hereditary fructose intolerance leading to liver and renal complications. These conditions highlight the importance of aldolase in maintaining metabolic health with implications for proteins within the same pathways potentially affecting their functions and expression levels.
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3 product images
Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (ab196994)
Typical NADH Standard Curve.
Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (ab196994)
Aldolase activity in rat kidney and muscle, HeLa and 3T3 cells. Data obtained performing assay as described in assay protocol.
Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (ab196994)
Relative aldolase activity in lysates prepared from rat kidney (14.2 μg), rat muscle (10.64 μg), 3T3 cells (9.97 μg) and HeLa cells (11.35 μg).
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