Aldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Colorimetric
Plasma, Tissue, Suspension cells, Serum, Adherent cells
Enzyme activity
Mammals
< 100 µU
ALDOC, ALDOA
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDB, ALDOB
Aldolase Activity Assay ab196994, aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDB, ALDOB
Colorimetric
Plasma, Tissue, Suspension cells, Serum, Adherent cells
Enzyme activity
Mammals
Microplate reader
< 100 µU
Blue Ice
-20°C
-20°C
-20°C
Aldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.
This assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.
This product is manufactured by BioVision, an Abcam company and was previously called K665 Aldolase Activity Colorimetric Assay Kit. K665-100 is the same size as the 100 test size of ab196994.
Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone.
There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.
Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.
This supplementary information is collated from multiple sources and compiled automatically.
Aldolase also known as fructose-bisphosphate aldolase is an enzyme that plays a critical role in glycolysis catalyzing the reversible cleavage of fructose 16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Aldolase is a homotetramer with a molecular mass of approximately 158 kDa. It is highly expressed in liver muscle and brain tissues. Aldolase consists of three isoforms: aldolase A B and C each predominant in different tissues contributing to tissue-specific roles within the organism.
Aldolase catalyzes an important reaction in energy metabolism ensuring the continuation of glycolytic flux which is essential for ATP production. Aldolases do not form part of larger protein complexes. However they interact with other enzymes within the glycolytic pathway to facilitate a smooth metabolic flow. Moreover aldolase A plays an additional role in gluconeogenesis the pathway involved in synthesizing glucose from non-carbohydrate sources.
Aldolase is an essential part of glycolysis and gluconeogenesis pathways. In glycolysis it partners with enzymes like phosphofructokinase and enolase to breakdown glucose for energy production. During gluconeogenesis aldolase works with enzymes including fructose-16-bisphosphatase to create glucose vital for maintaining blood sugar levels during fasting. Through these pathways aldolase ensures balance between energy-producing and energy-consuming states within the cell.
Mutations or deficiencies in aldolase particularly aldolase A have been linked to glycogen storage disease type XII which results in muscle weakness and exercise intolerance. Aldolase B deficiency is known to cause hereditary fructose intolerance leading to liver and renal complications. These conditions highlight the importance of aldolase in maintaining metabolic health with implications for proteins within the same pathways potentially affecting their functions and expression levels.
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Typical NADH Standard Curve.
Aldolase activity in rat kidney and muscle, HeLa and 3T3 cells. Data obtained performing assay as described in assay protocol.
Relative aldolase activity in lysates prepared from rat kidney (14.2 μg), rat muscle (10.64 μg), 3T3 cells (9.97 μg) and HeLa cells (11.35 μg).
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