Alkaline Phosphatase Assay Kit (Colorimetric)

• FuncS

Lab

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

ALP measured in cell culture supernatants showing activity (U) per L of tested sample. Samples were diluted 1-16 fold.

• Biochemical assay

Lab

Biochemical assay - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Diagram showing the principles of the Alkaline phosphatase assay method.

• FuncS

PubMed

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Effects of mineralocorticoid receptor blockade on phosphate-induced vascular calcification in vitro.

Alesutan I et al. (2017) measured alkaline phosphatase activity in vitro in primary human aortic smooth muscle cells using ab83369. Cells were treated with phosphate (pi) or without (Ctr, and with or without additional treatment of spironolactone (Spr) or 10 μM eplerenone (Epl).

Image courtesy of Alesutan I et al. Sci Rep. 2017; 7: 2059. doi: 10.1038/s41598-017-01882-2. Reproduced under the creative commons license http://creativecommons.org/licenses/by/4.0/.

• FuncS

PubMed

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Nepelska M et al. (2012) assayed alkaline phosphatase activity in Caco-2 cells after 48 hours treatment with PMA and Butyrate using Alkaline Phosphatase assay kit (ab83369).

Image from Nepelska M et al., PLoS One 7(12), fig 8c. Doi: 10.1371/journal.pone.0052869. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

PubMed

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Alkaline phosphatase activity measured in the study of osteoporosis.

Lee YM et al. (2019) used alkaline phosphatase assay kit ab83369 to measure the serum levels of alkaline phosphatase as a marker of bone turnover. Rat groups include sham-operated (Sham), OVX-control (OVX), E2-treated OVX groups (E2), and LABE 500 mg/kg treated OVX groups (LABE) (N=10/group).

Serum ALP levels in the OVX group were significantly increased compared to those in Sham group, and oral administration of LABE (500 mg/kg) significantly reduced the serum ALP levels as an osteoporosis-related serum marker.

The serum levels of alkaline phosphatase (ALP) were measured using assay kits (ab83369, Alkaline Phosphatase Assay Kit; Abcam) according to the manufacturer's instructions. Briefly, 80 μL of sample dilutions, 50 μL of 5 mM p-nitrophenyl phosphate (pNPP) solution, and 10 μL of ALP enzyme solution were added sequentially to the reaction wells and the plate was incubated at 25°C for 60 min protected from light. The reaction was halted by adding 20 μL stop solution, and then the absorbance was measured at 405 nm. ALP activity was calculated using a standard curve.

Image from Lee YM. et al., Biomed Res Int. 2019 Feb 19;2019:5073085. Fig 6b doi: 10.1155/2019/5073085. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Lab

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

ALP measured in biological fluids showing activity (U) per L of tested sample. Samples were diluted 4-64 fold.

• FuncS

PubMed

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

ALP activity in cells undergoing osteogenic differentiation.

Hermenean A et al. (2017) measured alkaline phosphatase activity in cells cultured in CHT/GO (3D chitosan scaffolds enriched with graphene oxide) biomaterials for up to 28 days using ab83369.

Image courtesy of Hermenean A et al. Sci Rep. 2017; 7: 16641. doi: 10.1038/s41598-017-16599-5

• FuncS

Lab

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• FuncS

Supplier Data

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Measurement of ALP activity using ab83369. a. ALP activity in fresh medium (80 μL, without culturing), 3 day old HeLa cell culture medium (80 μL) and human serum (80 μL, 1/10 dilution). b. ALP activity in HeLa cells. 5 x 10⁴ HeLa cells were homogenized in 1 mL of Assay Buffer, diluted 1/10 in Assay Buffer, and 80 μL used for the measurement.

• Schematic Diagram

Supplier Data

Schematic Diagram - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

Representative image of Alkaline Phosphatase Assay Kit (Colorimetric) ab83369

Components shown from left to right :

- pNPP Substrate

- Enzyme Mix II

- Stop Solution I

- ALP Assay Buffer I

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.