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Alkaline Phosphatase Assay Kit (Colorimetric) ab83369 is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and other mammalian samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Range

10 - 250 µU

Assay time

1h 10m

Sensitivity

> 10 µU

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Images

Target data

What's included?

10000 Test
Components
ALP Assay Buffer I
20 x 100 mL
Enzyme Mix II
20 x 1 Vial
Stop Solution I
20 x 10 mL
pNPP Substrate
20 x 1 Vial

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Alkaline Phosphatase Assay Kit (Colorimetric) ab83369 is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and other mammalian samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Range

10 - 250 µU

Assay time

1h 10m

Assay Platform

Microplate reader

Sensitivity

> 10 µU

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Alkaline Phosphatase Assay Kit (Colorimetric) ab83369 is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and other mammalian samples.

The kit is a pNPP assay; it uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP.

Alkaline phosphatase assay protocol summary:
- add samples and standards to wells
- add pNPP solution to sample wells (not to standards)
- add ALP enzyme solution to standard wells (not to samples)
- incubate for 60 min at room temp
- add stop solution
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K412 Alkaline Phosphatase Activity Colorimetric Assay Kit. K412-500 is the same size as the 500 test size of ab83369.

This kit contains 10 substrate tablets providing convenience for multiple usages.

pNPP Assays

pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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