Alkaline Phosphatase Assay Kit (Fluorometric) ab83371 is a highly sensitive kit to detect alkaline phosphatase (ALP) activity in serum and other biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomimetics (Basel, Switzerland) 8:2023Bio-Piezoelectric Ceramic Composites for Electroactive Implants-Biological Performance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeatriz Ferreira Fernandes,Neusa Silva,Joana Faria Marques,Mariana Brito Da Cruz,Laura Tiainen,Michael Gasik,Óscar Carvalho,Filipe Samuel Silva,João Caramês,António MataPubMed 37622943
- International journal of molecular sciences 24:2023Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D Concentration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUsman Rashid,Sandra K Becker,Gerhard Sponder,Susanne Trappe,Mansur A Sandhu,Jörg R AschenbachPubMed 37240030
- Journal of tissue engineering 14:204173142311597402023Repairing a critical cranial defect using WISP1-pretreated chondrocyte scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIdan Carmon,Shira Kalmus,Anna Zobrab,Michael Alterman,Raphaelle Emram,May Gussarsky,Leonid Kandel,Eli Reich,Nardi Casap,Mona Dvir-GinzbergPubMed 36949842
- International journal of implant dentistry 8:392022Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Baca-Gonzalez,Rebeca Serrano Zamora,Lisa Rancan,Francisco González Fernández-Tresguerres,Isabel Fernández-Tresguerres,Rosa M López-Pintor,Juan López-Quiles,Isabel Leco,Jesús TorresPubMed 36184700
- Acta biomaterialia 148:355-3732022Additive manufacturing of bioactive and biodegradable porous iron-akermanite composites for bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesN E Putra,K G N Borg,P J Diaz-Payno,M A Leeflang,M Klimopoulou,P Taheri,J M C Mol,L E Fratila-Apachitei,Z Huan,J Chang,J Zhou,A A ZadpoorPubMed 35690326
- Biomacromolecules 23:2803-28132022Sugar-Assisted Cryopreservation of Stem Cell-Laden Gellan Gum-Collagen Interpenetrating Network Hydrogels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Yao Ng,Kee Ying Fremi Tan,Pui Lai Rachel EePubMed 35675906
- Biomimetics (Basel, Switzerland) 7:2022Modification of Zirconia Implant Surfaces by Nd:YAG Laser Grooves: Does It Change Cell Behavior?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Brito da Cruz,Joana Faria Marques,Ana Filipa Silva Marques,Sara Madeira,Óscar Carvalho,Filipe Silva,João Caramês,António Duarte Sola Pereira da MataPubMed 35645176
- Materials (Basel, Switzerland) 15:2022The Effect of Low-Temperature Thermal Processing on Bovine Hydroxyapatite Bone Substitutes, toward Bone Cell Interaction and Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGemma Claire Porter,Dina Abdelmoneim,Kai Chun Li,Warwick John Duncan,Dawn Elizabeth CoatesPubMed 35407837
- Materials (Basel, Switzerland) 15:2022Human Gingival Fibroblast and Osteoblast Behavior on Groove-Milled Zirconia Implant Surfaces.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Brito da Cruz,Joana Faria Marques,Neusa Silva,Sara Madeira,Óscar Carvalho,Filipe Samuel Silva,João Caramês,António MataPubMed 35407819
- Cardiovascular research 119:302-3152022Age-related enhanced degeneration of bioprosthetic valves due to leaflet calcification, tissue crosslinking, and structural changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingfei Xue,Alexander P Kossar,Alexey Abramov,Antonio Frasca,Mingze Sun,Mariya Zyablitskaya,David Paik,David Kalfa,Mila Della Barbera,Gaetano Thiene,Satoshi Kozaki,Takayuki Kawashima,Joseph H Gorman,Robert C Gorman,Matthew J Gillespie,Chrystalle Katte Carreon,Stephen P Sanders,Robert J Levy,Giovanni FerrariPubMed 35020813
Key facts
- Detection method
Fluorescent
- Sample types
Saliva, Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
1h
Associated Products
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Target data
Function
This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).
Alternative names
AP-TNAP, TNSALP, Alkaline phosphatase liver/bone/kidney isozyme, ALPL
What's included?
Recommended products
Alkaline Phosphatase Assay Kit (Fluorometric) ab83371 is a highly sensitive kit to detect alkaline phosphatase (ALP) activity in serum and other biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
AP-TNAP, TNSALP, Alkaline phosphatase liver/bone/kidney isozyme, ALPL
Key facts
- Detection method
Fluorescent
- Sample types
Saliva, Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
1h
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371) is a highly sensitive kit to detect alkaline phosphatase (ALP) activity in serum and other biological samples. In the alkaline phosphatase assay protocol, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate, resulting in an increased fluorescent signal (Ex/Em = 360nm/440nm) when the substrate is dephosphorylated.
The kit is an ultra sensitive, simple, direct and HTS-ready assay designed to measure ALP activity with a detection sensitivity ~1 μU, more sensitive than colorimetric assays.
Alkaline phosphatase assay protocol summary:
- add samples and standards to wells
- add reaction mix to wells
- add ALP enzyme solution to standard wells (not to samples)
- incubate for 30 min at room temp
- add stop solution
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K422 Alkaline Phosphatase Activity Fluorometric Assay Kit. K422-500 is the same size as the 500 test size of ab83371.
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with many diseases in the liver and bones.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alkaline phosphatase also known as ALP is an enzyme with significant biological roles. It mechanically functions by catalyzing the hydrolysis of phosphate groups from various molecules including nucleotides proteins and alkaloids under alkaline conditions. ALP is a metalloenzyme possessing zinc and magnesium ions at its active site to aid in the reaction. This enzyme has a molecular mass of approximately 86 kDa and exists in dimeric form. ALP is expressed in many tissues with high levels in liver bone kidney and the placenta.
- Biological function summary
ALP plays an important role in maintaining mineral balance and bone metabolism by breaking down pyrophosphate an inhibitor of mineralization. It facilitates the regulation of phosphate metabolism by contributing to phosphorus and calcium homeostasis. ALP is not part of a larger complex but interacts with calcium and phosphate ions to aid in the formation and resorption of bone mineral tissue. It serves as a marker in bone growth and liver function due to its prominent activity in those tissues visible in assays like the ALP staining method used to gauge enzyme presence and action.
- Pathways
Alkaline phosphatase activity plays an essential role in the metabolic pathways related to bone mineralization and liver function. In bone tissue the enzyme participates in the calcification process important for bone strength and development by regulating inorganic phosphate availability. The liver expression of ALP is involved in bile excretion pathways where it dephosphorylates bile acids aiding in their conversion and recycling. It has a significant relationship with proteins such as osteocalcin in the bone mineralization pathway and bile acid transporters in the liver.
- Associated diseases and disorders
Alkaline phosphatase levels serve as diagnostic indicators for bone and liver diseases. High levels of ALP are often associated with bone disorders like Paget's disease and liver conditions like cholestasis. In these disorders ALP activity rises due to increased enzyme synthesis by osteoblasts in bone diseases or impaired bile flow in liver diseases. Furthermore the liver enzyme gamma-glutamyl transferase (GGT) is often measured alongside ALP to differentiate the cause of elevated ALP levels particularly to assess liver pathology.
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4 product images
Osta B et al., Front Immunol., 5:425. Figure 2.; doi:10.3389/fimmu.2014.00425. (2014)Functional Studies - Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371)
Effects of IL-17A and/or TNF-α on alkaline phosphatase activity are shown. hMSCs were plated at a density of 5 x 103 cell/cm2, cells were cultured in osteogenic medium for 14 days, in the absence (0) or presence of TNF-α 1 ng/ml and/or IL-17A 50 ng/ml. ALP activity was measured by fluorometry useing ab83371. Results were analyzed using the Wilcoxon test. ∗p < 0.05; ∗∗p < 0.005 vs. induction medium alone.
Functional Studies - Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371)
Alkaline phosphatase measured in mouse tissue lysates showing activity (mU) per mg protein of tested sample
Functional Studies - Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371)
Alkaline phosphatase measured in biological fluids showing quantity (mU) per microliter of tested sample
Functional Studies - Alkaline Phosphatase Assay Kit (Fluorometric) (ab83371)
4-Methylumbelliferone (4-MU) standard curve.
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