AB242286

Alkaline Phosphatase Staining Kit (Red)

Name: Alkaline Phosphatase Staining Kit (Red) (ab242286)| Abcam
Brand: Abcam Limited
SKU: AB242286
Price: 595 USD
Availability: InStock

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(13 Publications)

Alkaline Phosphatase Staining Kit (Red) (ab242286) uses a convenient method to identify and monitor stem cells by staining for alkaline phosphatase (AP) activity in immunocytochemistry (ICC).

- Use with human and murine embryonic stem cells (ESC) and embryonic germ cells (EG)

View Alternative Names

ALPPL, ALPPL2, ALPG, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like, GCAP, PLAP-like

1 Images

Supplier Data

Immunocytochemistry - Alkaline Phosphatase Staining Kit (Red) (AB242286)

AP staining of ES Cells.

Murine embryonic stem cells (ES-D3) are maintained in an undifferentiated stage on gelatin-coated dishes in the presence of LIF, as indicated by the high AP activity. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, AP staining of undifferentiated cells was performed as described in the Assay Protocol.

Key facts

Detection method

Colorimetric

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Reactivity data

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Product details

Alkaline Phosphatase Staining Kit (Red) (ab242286) uses a convenient method to identify and monitor stem cells by staining for alkaline phosphatase activity.

Pluripotent stem cells typically have higher levels of alkaline phosphatase activity than differentiated cell types (although osteoblasts retain this activity). Alkaline phosphatase staining / activity is often used as a biomarker of stem cells.

In this alkaline phosphatase staining kit, alkaline phosphatase acts on a substrate to form a red colored chemical, producing a red color in stem cells that is imaged with light microscopy.

The kit can be used with any tissue section or cell type that have alkaline phosphatase activity. It is not compatible with paraffin-embedded sections.

The alkaline phosphatase staining protocol is simple:
- prepare samples (the kit protocol is written for use with ES cells; the kit can be used with any cell or tissues with alkaline phosphatase activity)
- add fixing solution to cells or tissues, incubate for 2 mins, and wash with PBST
- add staining solution and incubate for 15-30 mins
- wash with PBS and image

Alkaline Phosphatase Staining Kit (Purple) ab242287 is an alternative to this kit, using an equivalent method, with a different colored dye. Generally, this kit gives cleaner staining.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242286-100Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Fixing Solution", "number":"AB242286-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"AP Staining Solution B", "number":"AB242286-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"AP Staining Solution A", "number":"AB242286-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.

Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.

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Product protocols

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPG

Additional targets

ALPP,Alkaline phosphatase, tissue-nonspecific isozyme,ALPI

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Publications (13)

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In Vitro Evaluation of the Effects of Hyaluronic Acid and an Aminoacidic Pool on Human Osteoblasts.

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HMCES modulates the transcriptional regulation of nodal/activin and BMP signaling in mESCs.

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Tao Liang,Jianbo Bai,Wei Zhou,Hao Lin,Shixin Ma,Xuechen Zhu,Qinghua Tao,Qiaoran Xi

Experimental biology and medicine (Maywood, N.J.) 247:1379-1387 PubMed35611795

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Mouse embryonic stem cells require multiple amino acids.

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Boyang Zhang,Jian Feng

Frontiers in cell and developmental biology 10:820255 PubMed35652095

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Esrrb Regulates Specific Feed-Forward Loops to Transit From Pluripotency Into Early Stages of Differentiation.

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Amin R Mazloom,Huilei Xu,Jaume Reig-Palou,Ana Vasileva,Angel-Carlos Román,Sonia Mulero-Navarro,Ihor R Lemischka,Ana Sevilla

View all publications

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Alkaline Phosphatase Staining Kit (Red)

Immunocytochemistry - Alkaline Phosphatase Staining Kit (Red) (AB242286)

Key facts

Detection method

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (13)

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