Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.
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- Pharmaceuticals (Basel, Switzerland) 17:2024The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen LeePubMed 38256924
- Antioxidants (Basel, Switzerland) 12:2023In Vitro α-Glucosidase and α-Amylase Inhibition, Cytotoxicity and Free Radical Scavenging Profiling of the 6-Halogeno and Mixed 6,8-Dihalogenated 2-Aryl-4-methyl-1,2-dihydroquinazoline 3-Oxides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNontokozo M Magwaza,Garland K More,Samantha Gildenhuys,Malose J MphahlelePubMed 38001824
- Molecules (Basel, Switzerland) 28:2023Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon TangPubMed 37513196
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
Target data
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Targets
AMY2A
Alternative names
Alpha-amylase 1A, Salivary alpha-amylase, AMY1A, AMY1
What's included?
Recommended products
Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.
Alternative names
Alpha-amylase 1A, Salivary alpha-amylase, AMY1A, AMY1
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.
Alpha-Amylase enzyme is responsible for the breakdown of complex carbohydrates in humans. Thus, inhibition of α-amylase could be considered as a strategy for the treatment of disorders related to carbohydrate uptake, such as diabetes, obesity, dental cavities and periodontal diseases. In this kit human Alpha-Amylase hydrolyzes the synthetic substrate, yielding smaller fragments containing the chromophore (pNP; OD = 405 nm). A potent, specific inhibitor is also included in the kit.
The 1-step assay is simple and can be completed within 1 hour.
This product is manufactured by BioVision, an Abcam company and was previously called K482 a-Amylase Inhibitor Screening Kit. K482-100 is the same size as the 100 test size of ab283391.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pancreatic alpha amylase also known as alpha-amylase P or AMY2A and salivary alpha amylase (AMY1) are enzymes that play essential roles in carbohydrate digestion. Mechanically they hydrolyze alpha-14-glycosidic linkages in starches breaking them down into smaller sugars like maltose and dextrins. Pancreatic alpha amylase has a molecular mass of approximately 56 kDa. The pancreas and salivary glands predominantly express these enzymes facilitating digestive processes in the small intestine and oral cavity respectively.
- Biological function summary
These alpha-amylases initiate the digestive process for dietary starches and glycogen by breaking them into sugars which are then more easily absorbed by the body. While they function largely independently their activity is supported by other digestive enzymes that further process carbohydrates throughout the digestive system. This enzymatic action is important for maintaining energy supply from glycogen and starches highlighting the significant role of alpha amylase in metabolic nutrition.
- Pathways
The catalytic action of pancreatic and salivary alpha amylase is a part of the digestive pathway for carbohydrates where it accelerates the breakdown of starches. These enzymes interact within the amylase pathway which includes other related enzymes like alpha-glucosidases that sequentially digest disaccharides into glucose. They are not known to form permanent complexes but work in conjunction with these digestive enzymes to facilitate efficient energy release.
- Associated diseases and disorders
Alterations in alpha-amylase activity have connections to conditions like pancreatitis and salivary gland dysfunctions where changes in enzyme levels provide important diagnostic information. Conditions such as pancreatitis an inflammation of the pancreas link directly to abnormal amylase activity and concentration. Additionally they associate with other proteins involved in inflammation and stress responses which are measured in clinical settings to assess pancreatic and salivary gland health.
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1 product image
Functional Studies - Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391)
Inhibition of Α−Amylase, Human Salivary (blue square) and Pancreas (green dot) by Α−Amylase Inhibitor. IC50 of Α−Amylase Inhibitor were calculated to be 108± 5 ng and 267 ± 4 ng, respectively.
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