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AB283391

Alpha-Amylase Inhibitor Screening Kit (Colorimetric)

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(6 Publications)

Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.

View Alternative Names

AMY1, AMY1A, Alpha-amylase 1A, Salivary alpha-amylase, Pancreatic alpha-amylase, PA, AMY2A

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Functional Studies - Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (AB283391)
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Supplier Data

Functional Studies - Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (AB283391)

Inhibition of Α−Amylase, Human Salivary (blue square) and Pancreas (green dot) by Α−Amylase Inhibitor. IC50 of Α−Amylase Inhibitor were calculated to be 108± 5 ng and 267 ± 4 ng, respectively.

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.

Alpha-Amylase enzyme is responsible for the breakdown of complex carbohydrates in humans. Thus, inhibition of alpha-amylase could be considered as a strategy for the treatment of disorders related to carbohydrate uptake, such as diabetes, obesity, dental cavities and periodontal diseases. In this kit human Alpha-Amylase hydrolyzes the synthetic substrate, yielding smaller fragments containing the chromophore (pNP; OD = 405 nm). A potent, specific inhibitor is also included in the kit.

The 1-step assay is simple and can be completed within 1 hour.

Other Notes
This product was previously called K482 a-Amylase Inhibitor Screening Kit. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab283391-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"α-Amylase Substrate", "number":"AB283391-CMP04", "productcode":"" }, { "size":"1 x 55 mL", "name":"Amylase Assay Buffer", "number":"AB283391-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha-Amylase, Human Saliva", "number":"AB283391-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"α-Amylase Inhibitor", "number":"AB283391-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic alpha amylase also known as alpha-amylase P or AMY2A and salivary alpha amylase (AMY1) are enzymes that play essential roles in carbohydrate digestion. Mechanically they hydrolyze alpha-14-glycosidic linkages in starches breaking them down into smaller sugars like maltose and dextrins. Pancreatic alpha amylase has a molecular mass of approximately 56 kDa. The pancreas and salivary glands predominantly express these enzymes facilitating digestive processes in the small intestine and oral cavity respectively.
Biological function summary

These alpha-amylases initiate the digestive process for dietary starches and glycogen by breaking them into sugars which are then more easily absorbed by the body. While they function largely independently their activity is supported by other digestive enzymes that further process carbohydrates throughout the digestive system. This enzymatic action is important for maintaining energy supply from glycogen and starches highlighting the significant role of alpha amylase in metabolic nutrition.

Pathways

The catalytic action of pancreatic and salivary alpha amylase is a part of the digestive pathway for carbohydrates where it accelerates the breakdown of starches. These enzymes interact within the amylase pathway which includes other related enzymes like alpha-glucosidases that sequentially digest disaccharides into glucose. They are not known to form permanent complexes but work in conjunction with these digestive enzymes to facilitate efficient energy release.

Alterations in alpha-amylase activity have connections to conditions like pancreatitis and salivary gland dysfunctions where changes in enzyme levels provide important diagnostic information. Conditions such as pancreatitis an inflammation of the pancreas link directly to abnormal amylase activity and concentration. Additionally they associate with other proteins involved in inflammation and stress responses which are measured in clinical settings to assess pancreatic and salivary gland health.
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Product protocols

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Target data

Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed : 12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed : 12527308).
See full target information AMY1A

Additional targets

Pancreatic alpha-amylase
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 14: PubMed39856950

2025

, and as Potential Functional Food Ingredients for the Management of Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Emer Shannon,Maria Hayes

Antioxidants (Basel, Switzerland) 13: PubMed39456508

2024

Exploring the 3,5-Dibromo-4,6-dimethoxychalcones and Their Flavone Derivatives as Dual α-Glucosidase and α-Amylase Inhibitors with Antioxidant and Anticancer Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Jackson K Nkoana,Malose J Mphahlele,Garland K More,Yee Siew Choong

In silico pharmacology 12:9 PubMed38327875

2024

Investigation of alpha amylase inhibitors from L. by in silico and in vitro studies.

Applications

Unspecified application

Species

Unspecified reactive species

Akshay Galagali,Vishal S Patil,Kashinath Hiremath,Ganesh H Sampat,Rajlaxmi Patil,Rajashri Virge,Darasaguppe R Harish,Harsha V Hedge,Subarna Roy

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256924

2024

The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen Lee

Antioxidants (Basel, Switzerland) 12: PubMed38001824

2023

In Vitro α-Glucosidase and α-Amylase Inhibition, Cytotoxicity and Free Radical Scavenging Profiling of the 6-Halogeno and Mixed 6,8-Dihalogenated 2-Aryl-4-methyl-1,2-dihydroquinazoline 3-Oxides.

Applications

Unspecified application

Species

Unspecified reactive species

Nontokozo M Magwaza,Garland K More,Samantha Gildenhuys,Malose J Mphahlele

Molecules (Basel, Switzerland) 28: PubMed37513196

2023

Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Anas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon Tang
View all publications
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