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AB239716

Alpha Galactosidase Activity Assay Kit

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(1 Publication)

Alpha Galactosidase Activity Assay Kit ab239716 is a fast and accurate assay for the measurement of total alpha galactosidase activity. Readout on any fluorometric (Ex/Em= 360/445 nm) plate reader.

- Kit includes standard curve for quantitation, and positive control enzyme
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Alpha-galactosidase A, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidase GLA, Melibiase, GLA

4 Images
Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)
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Supplier Data

Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)

4-Methylumbelliferon Standard Curve

Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)
  • FuncS

Supplier Data

Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)

Measurement of α-Galactosidase Acitvity in undiluted human pooled saliva (5 µL)

Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)
  • FuncS

Supplier Data

Functional Studies - Alpha Galactosidase Activity Assay Kit (AB239716)

α-Galactosidase Activity in Mouse kidney tissue extracts (1 µg protein) and U937 cell lysate (0.2 µg protein)

Other - Alpha Galactosidase Activity Assay Kit (AB239716)
  • Other

Supplier Data

Other - Alpha Galactosidase Activity Assay Kit (AB239716)

Representative image of Alpha Galactosidase Activity Assay Kit ab239716

Components shown from left to right :

- alpha-Gal Substrate

- 4-Methyumbelliferone Standard

- alpha-Gal Positive Control

- Assay Buffer 25

- Stop Solution V

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Tissue Homogenate, Serum, Cell Lysate

Assay Platform

Microplate reader

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alpha Galactosidase Activity Assay Kit ab239716 provides a simple, rapid way to monitor total alpha galactosidase (alpha-Gal) activity in a wide variety of biological samples.

How the assay works
In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 360/445 nm). The assay is specific, sensitive and can detect as low as 0.1 μU of α-Galactosidase activity. This kit does not detect beta galactosidase activity.

Alpha Galactosidase assay methods
There are two methods used for alpha galactosidase assays:

The most common method uses 4-Methylumbelliferyl-alpha-D-galactopyranoside, as an alpha galactosidase substrate. Alpha galactosidase cleaves the substrate releasing fluorescent Methylumbelliferone. This is the method used in alpha galactosidase assay kit ab239716.

The alternative, less-popular method uses the substrate 4-nitrophenyl-α D galacto-pyranoside. Similarly, alpha galactosidase cleaves the substrate releasing pNA, which can be detected by absorbance.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab239716-2000Test", "assetComponentDetails": [ { "size":"20 x 25 mL", "name":"Assay Buffer 25", "number":"AB239716-CMP02" }, { "size":"20 x 1 Vial", "name":"alpha-Gal Positive Control", "number":"AB239716-CMP03" }, { "size":"20 x 35 µL", "name":"4-Methylumbelliferone Standard", "number":"AB239716-CMP01" }, { "size":"20 x 220 µL", "name":"α-Gal Substrate", "number":"AB239716-CMP05" }, { "size":"20 x 25 mL", "name":"Stop Solution V", "number":"AB239716-CMP04" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab239716-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 25", "number":"AB239716-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha-Gal Positive Control", "number":"AB239716-CMP03", "productcode":"" }, { "size":"1 x 35 µL", "name":"4-Methylumbelliferone Standard", "number":"AB239716-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Stop Solution V", "number":"AB239716-CMP04", "productcode":"" }, { "size":"1 x 220 µL", "name":"α-Gal Substrate", "number":"AB239716-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.
Biological function summary

The role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.

Pathways

The involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.

Defects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.
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Product protocols

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Target data

Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
See full target information GLA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering (Basel, Switzerland) 10: PubMed37627798

2023

Efficient Decellularization of the Full-Thickness Rat-Derived Abdominal Wall to Produce Acellular Biologic Scaffolds for Tissue Reconstruction: Promising Evidence Acquired from In Vitro Results.

Applications

Unspecified application

Species

Unspecified reactive species

George Skepastianos,Panagiotis Mallis,Epameinondas Kostopoulos,Efstathios Michalopoulos,Vasileios Skepastianos,Chrysoula Palazi,Lucia Pannuto,Gerasimos Tsourouflis
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websiteProtocolBooklet
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Product promise

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