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AB174093

Alpha-Glucosidase Activity Assay Kit (Colorimetric)

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(2 Publications)

In Alpha-Glucosidase Activity Assay Kit ab174093, α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA, G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, Neutral alpha-glucosidase C, GANC

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Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)
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Lab

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)

Time course of Alpha-Glucosidase Activity in various samples

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)
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Lab

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)
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Supplier Data

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)

α-Glucosidase kinetic assay.

Kinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset : Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)
  • FuncS

Lab

Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)

Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate

Other - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)
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Supplier Data

Other - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (AB174093)

Representative image of Alpha-Glucosidase Activity Assay Kit (Colorimetric) ab174093

Components shown from left to right :

- alpha-Glucosidase Positive Control

- alpha-Glucosidase Substrate Mix

- p-Nitrophenol Standard

- alpha-Glucosidase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Tissue, Serum, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Range

0.1 - 10 mU/well

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K690 α-Glucosidase Activity Colorimetric Assay Kit. K690-100 is the same size as the 100 test size of ab174093.

α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.

Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab174093-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"alpha-Glucosidase Positive Control", "number":"AB174093-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"α-Glucosidase Assay Buffer", "number":"AB174093-CMP01", "productcode":"" }, { "size":"1 x 300 µL", "name":"α-Glucosidase Substrate Mix", "number":"AB174093-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"p-Nitrophenol Standard", "number":"AB174093-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-glucosidase also known as alpha-14-glucosidase is an enzyme with a mass of approximately 105 kDa. It plays an important role in carbohydrate metabolism by catalyzing the hydrolysis of alpha-glycosidic bonds releasing glucose from the non-reducing end of glycogen. This enzyme is mostly present in the brush border of the small intestine where it facilitates the final step of complex carbohydrate digestion. Its activity is essential for the conversion of disaccharides and oligosaccharides into glucose and tools like alpha-glucosidase assays help in quantifying this activity.
Biological function summary

Alpha-glucosidase activity is important for maintaining normal glucose levels in the blood. It forms part of a larger enzyme complex that operates along the intestinal microvilli. Within the complex it collaborates with other hydrolases to optimize disaccharide breakdown. This role ensures the efficient transformation of ingested carbohydrates into absorbable monosaccharides which is an important cellular energy source.

Pathways

Alpha-glucosidase participates in the digestive process pathways notably the starch and sucrose metabolism pathway. It acts downstream of amylase enzymes that break down polysaccharides. This enzyme works closely with other glucosidases and maltase-glucoamylase within these pathways to terminate the carbohydrate digestion. Proteins like sucrase-isomaltase also interact in these pathways enhancing carbohydrate assimilation.

Alpha-glucosidase is associated mainly with Pompe disease and diabetes mellitus. Pompe disease results from a defect in the related protein acid alpha-glucosidase (GAA) leading to glycogen accumulation in lysosomes. In diabetes inhibiting intestinal alpha-glucosidase reduces postprandial blood glucose levels making it a target for therapeutic drugs. Understanding these connections aids in developing treatments that modulate enzyme activity to manage carbohydrate metabolism disorders.
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Product protocols

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Target data

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).
See full target information Lysosomal alpha-glucosidase

Additional targets

GANAB,GANC
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 20:98 PubMed35236363

2022

Effects of iron oxide nanoparticles as T-MRI contrast agents on reproductive system in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Heyu Yang,Hui Wang,Chenghao Wen,Shun Bai,Pengfei Wei,Bo Xu,Yunjun Xu,Chaozhao Liang,Yunjiao Zhang,Guilong Zhang,Huiqin Wen,Li Zhang

Physiological genomics 52:203-216 PubMed32146873

2020

Hepatic proteome analysis reveals altered mitochondrial metabolism and suppressed acyl-CoA synthetase-1 in colon-26 tumor-induced cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Andy V Khamoui,Dorota Tokmina-Roszyk,Harry B Rossiter,Gregg B Fields,Nishant P Visavadiya
View all publications
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Product promise

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