In Alpha-Glucosidase Activity Assay Kit ab174093, ?-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Journal of nanobiotechnology 20:982022Effects of iron oxide nanoparticles as T-MRI contrast agents on reproductive system in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeyu Yang,Hui Wang,Chenghao Wen,Shun Bai,Pengfei Wei,Bo Xu,Yunjun Xu,Chaozhao Liang,Yunjiao Zhang,Guilong Zhang,Huiqin Wen,Li ZhangPubMed 35236363
- Physiological genomics 52:203-2162020Hepatic proteome analysis reveals altered mitochondrial metabolism and suppressed acyl-CoA synthetase-1 in colon-26 tumor-induced cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndy V Khamoui,Dorota Tokmina-Roszyk,Harry B Rossiter,Gregg B Fields,Nishant P VisavadiyaPubMed 32146873
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Cell culture extracts, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Range
0.1 - 10 mU/well
Target data
Function
Essential for the degradation of glycogen in lysosomes (PubMed:1856189, PubMed:7717400, PubMed:14695532, PubMed:18429042). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).
Targets
GANAB, GANC
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
What's included?
Recommended products
In Alpha-Glucosidase Activity Assay Kit ab174093, ?-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Cell culture extracts, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Range
0.1 - 10 mU/well
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
In Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K690 α-Glucosidase Activity Colorimetric Assay Kit. K690-100 is the same size as the 100 test size of ab174093.
α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase.
Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-glucosidase also known as alpha-14-glucosidase is an enzyme with a mass of approximately 105 kDa. It plays an important role in carbohydrate metabolism by catalyzing the hydrolysis of alpha-glycosidic bonds releasing glucose from the non-reducing end of glycogen. This enzyme is mostly present in the brush border of the small intestine where it facilitates the final step of complex carbohydrate digestion. Its activity is essential for the conversion of disaccharides and oligosaccharides into glucose and tools like alpha-glucosidase assays help in quantifying this activity.
- Biological function summary
Alpha-glucosidase activity is important for maintaining normal glucose levels in the blood. It forms part of a larger enzyme complex that operates along the intestinal microvilli. Within the complex it collaborates with other hydrolases to optimize disaccharide breakdown. This role ensures the efficient transformation of ingested carbohydrates into absorbable monosaccharides which is an important cellular energy source.
- Pathways
Alpha-glucosidase participates in the digestive process pathways notably the starch and sucrose metabolism pathway. It acts downstream of amylase enzymes that break down polysaccharides. This enzyme works closely with other glucosidases and maltase-glucoamylase within these pathways to terminate the carbohydrate digestion. Proteins like sucrase-isomaltase also interact in these pathways enhancing carbohydrate assimilation.
- Associated diseases and disorders
Alpha-glucosidase is associated mainly with Pompe disease and diabetes mellitus. Pompe disease results from a defect in the related protein acid alpha-glucosidase (GAA) leading to glycogen accumulation in lysosomes. In diabetes inhibiting intestinal alpha-glucosidase reduces postprandial blood glucose levels making it a target for therapeutic drugs. Understanding these connections aids in developing treatments that modulate enzyme activity to manage carbohydrate metabolism disorders.
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4 product images
Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate
Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
Time course of Alpha-Glucosidase Activity in various samples
Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093)
α-Glucosidase kinetic assay.
Kinetic profile of various amounts (0, 2, 4, 6, 8 & 10 mU) of α-glucosidase run at 25°C under this protocol. Inset: Results for 0-0.2-0.4-0.6-0.8-1.0 mU of α-glucosidase. Data points after 5 minutes were used to determine slope. This is example data only.
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