The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
- Pharmaceuticals (Basel, Switzerland) 17:2024The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen LeePubMed 38256924
- Molecules (Basel, Switzerland) 28:2023Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon TangPubMed 37513196
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
Target data
Function
Essential for the degradation of glycogen in lysosomes (PubMed:1856189, PubMed:7717400, PubMed:14695532, PubMed:18429042). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
What's included?
Recommended products
The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
The α-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme. It utilizes the ability of an active α-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an α-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.
The assay kit provides a rapid, simple and reliable test for high-throughput screening of α-Glucosidase inhibitors.
This product is manufactured by BioVision, an Abcam company and was previously called K938 a-Glucosidase Inhibitor Screening Kit (Colorimetric). K938-100 is the same size as the 100 test size of ab284520.
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1 product image
Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (ab284520)
Inhibition of alpa-Glucosidase activity by Acarbose.
α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.
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