The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- Pharmaceuticals (Basel, Switzerland) 17:2024The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen LeePubMed 38256924
- Molecules (Basel, Switzerland) 28:2023Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon TangPubMed 37513196
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
Target data
Function
Essential for the degradation of glycogen in lysosomes (PubMed:1856189, PubMed:7717400, PubMed:14695532, PubMed:18429042). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
What's included?
Recommended products
The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
Key facts
- Detection method
Colorimetric
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
The α-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme. It utilizes the ability of an active α-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an α-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.
The assay kit provides a rapid, simple and reliable test for high-throughput screening of α-Glucosidase inhibitors.
This product is manufactured by BioVision, an Abcam company and was previously called K938 a-Glucosidase Inhibitor Screening Kit (Colorimetric). K938-100 is the same size as the 100 test size of ab284520.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (ab284520)
Inhibition of alpa-Glucosidase activity by Acarbose.
α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com