JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB284520

alpha-Glucosidase Inhibitor Screening Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(3 Publications)

The alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520 is a convenient assay for the screening/characterizing Alpha-Glucosidase inhibitors.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA

2 Images
Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)
  • FuncS

Supplier Data

Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)

Inhibition of alpha-Glucosidase activity by Acarbose.

α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.

Other - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)
  • Other

Supplier Data

Other - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)

Representative image of alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520

Components shown from left to right :

- alpha-Glucosidase Positive Control

- alpha-Glucosidase Substrate Mix

- Acarbose

- alpha-Glucosidase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB283391

Alpha-Amylase Inhibitor Screening Kit (Colorimetric)

Functional Studies - Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (AB283391)

  • 0
  • 0
Assay Kits

AB102523

Amylase Assay Kit (Colorimetric)

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB204722

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520 is a convenient assay for the screening/characterizing Alpha-Glucosidase inhibitors.

How the assay works
The alpha-Glucosidase assay uses the ability of an active Alpha-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an Alpha-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.

Alpha-Glucosidase assay summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at OD: 410 nm in kinetic mode for 60 min at room temperature.

Other Notes
This product was previously called K938 Biovision a-Glucosidase Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284520-100Test", "assetComponentDetails": [ { "size":"1 x 4 Vial", "name":"alpha-Glucosidase Positive Control", "number":"AB284520-CMP02", "productcode":"AB325890" }, { "size":"1 x 300 µL", "name":"α-Glucosidase Substrate Mix", "number":"AB284520-CMP04", "productcode":"" }, { "size":"1 x 140 µL", "name":"Acarbose", "number":"AB284520-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"α-Glucosidase Assay Buffer", "number":"AB284520-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).
See full target information Lysosomal alpha-glucosidase
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 22: PubMed39728150

2024

Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from : Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent.

Applications

Unspecified application

Species

Unspecified reactive species

Navindu Dinara Gajanayaka,Eunyoung Jo,Minthari Sakethanika Bandara,Svini Dileepa Marasinghe,Chinmayee Bawkar,Yeon-Ju Lee,Gun-Hoo Park,Chulhong Oh,Youngdeuk Lee

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256924

2024

The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen Lee

Molecules (Basel, Switzerland) 28: PubMed37513196

2023

Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Anas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon Tang
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com