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The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity (quantitative)

Target data

Function

Essential for the degradation of glycogen in lysosomes (PubMed:1856189, PubMed:7717400, PubMed:14695532, PubMed:18429042). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).

Alternative names

What's included?

100 Test
Components
Acarbose
1 x 140 µL
α-Glucosidase Assay Buffer
1 x 25 mL
α-Glucosidase Positive Control
1 x 1 Vial
α-Glucosidase Substrate Mix
1 x 300 µL

Recommended products

The ?-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

The α-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme. It utilizes the ability of an active α-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an α-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.

The assay kit provides a rapid, simple and reliable test for high-throughput screening of α-Glucosidase inhibitors.

This product is manufactured by BioVision, an Abcam company and was previously called K938 a-Glucosidase Inhibitor Screening Kit (Colorimetric). K938-100 is the same size as the 100 test size of ab284520.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (ab284520), expandable thumbnail

    Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (ab284520)

    Inhibition of alpa-Glucosidase activity by Acarbose.

    α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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