AB83431

Alpha Ketoglutarate (alpha KG) Assay Kit

Name: Alpha Ketoglutarate (alpha KG) Assay Kit. Quantitative. ab83431 | Abcam
Brand: Abcam Limited
SKU: AB83431
Price: 715 USD
Availability: InStock

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(61 Publications)

Alpha Ketoglutarate (alpha KG) Assay Kit is a mix-and-read assay with one 30-min incubation step. It provides a simple, sensitive and rapid means for quantifying alpha KG.

- Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

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Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

FuncS

Lab

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

FuncS

Lab

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue, Suspension cells, Adherent cells

Results type

Quantitative

Sensitivity

= 0.01 nmol/well

Range

0.01 - 10 nmol/well

Assay time

30m

Assay Platform

Microplate reader

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Product details

Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.

Alpha ketoglutarate assay principle
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.

Alpha Ketoglutarate assay protocol summary
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader

Other Notes
This product was previously called K677 Biovision Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83431-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"a-KG Converting Enzyme", "number":"AB83431-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB83431-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"α-KG Standard", "number":"AB83431-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"alpha-KG Assay Buffer", "number":"AB83431-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83431-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-ketoglutarate sometimes called alpha-ketoglutaric acid or A-KG is an important intermediate in several metabolic processes. It plays a mechanical role in the Krebs cycle also known as the citric acid cycle where it functions to transfer energy through oxidative reactions. A-KG is a five-carbon molecule with a mass of approximately 146.11 g/mol and is primarily expressed in the mitochondria of cells particularly within tissues with high metabolic activity such as liver and muscle.

Biological function summary

Alpha-ketoglutarate supports amino acid metabolism and the regulation of gene expression. It acts as an essential nitrogen transporter in biochemical pathways. A-KG serves as a co-substrate for several dioxygenases including prolyl hydroxylases which are enzymes connected to collagen synthesis and the hypoxia-inducible factor pathway. Additionally it is involved in the formation of neurotransmitters and the detoxification of ammonia in animals.

Pathways

Alpha-ketoglutarate is deeply integrated within the tricarboxylic acid cycle and the gamma-aminobutyric acid (GABA) shunt. Within the TCA cycle A-KG forms important energy carriers like NADH and FADH2 impacting energy production. In the GABA shunt it closely associates with glutamate dehydrogenase which transforms glutamate emphasizing its role in nitrogen and energy balance. A-KG interacts with proteins like succinyl-CoA synthetase and is linked with enzymes such as isocitrate dehydrogenase.

Alterations in alpha-ketoglutarate levels have been linked to metabolic disorders like hyperammonemia and certain types of cancer. In hyperammonemia improper detoxification processes through the urea cycle can lead to elevated ammonia levels a problem in which alpha-ketoglutarate plays a detoxifying role. Oncogenic mutations in isocitrate dehydrogenase can lead to abnormal A-KG levels contributing to cancer progression by affecting cellular metabolism and gene expression.

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Product protocols

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Target data

See full target information 2-Oxoglutaric acid

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Publications (61)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:160 PubMed40204725

2025

O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sijia Fang,Guoqing Zhu,Yi Xie,Miao Ding,Ni Zhen,Jiabei Zhu,Siwei Mao,Xiaochen Tang,Han Wu,Qi Zhang,Aijia Zhang,Xin Ni,Qiuhui Pan,Ji Ma

Journal of molecular neuroscience : MN 75:46 PubMed40199799

2025

Fucoxanthinol Mitigates the Cytotoxic Effect of Chlorpyrifos and MPTP on the Dopaminergic Differentiation of SH-SY5Y Human Neuroblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ekramy M Elmorsy,Ayat B Al-Ghafari,Huda A Al Doghaither,Mona M Elghareeb,Mouhamed Alsaqati

Cell death & disease 16:192 PubMed40113747

2025

PER2 interaction with HSP70 promotes cuproptosis in oral squamous carcinoma cells by decreasing AKT stability.

Applications

Unspecified application

Species

Unspecified reactive species

Wenguang Yu,Shilin Yin,Hong Tang,Hengyan Li,Zhiwei Zhang,Kai Yang

Life metabolism 3:loae002 PubMed39872214

2025

IDH1 mutation inhibits differentiation of astrocytes and glioma cells with low oxoglutarate dehydrogenase expression by disturbing α-ketoglutarate-related metabolism and epigenetic modification.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanlin Zhao,Ying Yang,Risheng Yang,Chao Sun,Xing Gao,Xiwen Gu,Yuan Yuan,Yating Nie,Shenhui Xu,Ruili Han,Lijun Zhang,Jing Li,Peizhen Hu,Yingmei Wang,Huangtao Chen,Xiangmei Cao,Jing Wu,Zhe Wang,Yu Gu,Jing Ye

Immunology 174:450-461 PubMed39829079

2025

Mechanism Study of E2F8 Activation of SPC25-Mediated Glutamine Metabolism Promoting Immune Escape in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Machang Luo,Lingyan Xie,Baoyan Lin,Xia Su,Rongzhang Liang,Zhiyi Ma,Youtang Li

International journal of molecular sciences 25: PubMed39337535

2024

OXGR1-Dependent (Pro)Renin Receptor Upregulation in Collecting Ducts of the Clipped Kidney Contributes to Na Balance in Goldblatt Hypertensive Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Cárdenas,Camila Nuñez-Allimant,Katherin Silva,Catalina Cid-Salinas,Allison C León,Zoe Vallotton,Ramón A Lorca,Lilian Caroline Gonçalves de Oliveira,Dulce E Casarini,Carlos Céspedes,Minolfa C Prieto,Alexis A Gonzalez

Zoological research 45:1175-1187 PubMed39318125

2024

IDH2 and GLUD1 depletion arrests embryonic development through an H4K20me3 epigenetic barrier in porcine parthenogenetic embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Lin Zhan,Qin-Yue Lu,Song-Hee Lee,Xiao-Han Li,Ji-Dam Kim,Gyu-Hyun Lee,Jae-Min Sim,Hyeon-Ji Song,Ying-Yan Jin,Xiang-Shun Cui

Frontiers in molecular biosciences 11:1460987 PubMed39297074

2024

Recent progress of methods for cuproptosis detection.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Zhang,Ruiting Deng,Raoqing Guo,Yawen Jiang,Yichen Guan,Caiyue Chen,Wudi Zhao,Guobin Huang,Lian Liu,Hongli Du,Dongsheng Tang

Molecular medicine (Cambridge, Mass.) 30:143 PubMed39256649

2024

IDH2 regulates macrophage polarization and tumorigenesis by modulating mitochondrial metabolism in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Woo Lee,Soyoon Kim,Bokyung Kim,Jung Bae Seong,Young-Ho Park,Hong Jun Lee,Dong Kyu Choi,Eunbyul Yeom,Dong-Seok Lee

Cellular and molecular life sciences : CMLS 81:375 PubMed39212717

2024

Downregulation of malic enzyme 3 facilitates progression of gastric carcinoma via regulating intracellular oxidative stress and hypoxia-inducible factor-1α stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Huang,Yan Yang,Xiangliu Chen,Siying Zeng,Yiran Chen,Haiyong Wang,Xiadong Lv,Xun Hu,Lisong Teng

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Alpha Ketoglutarate (alpha KG) Assay Kit

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Key facts

Detection method

Sample types

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (61)

Product promise