Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
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- EMBO reports 24:e560092023HDAC6 deacetylates IDH1 to promote the homeostasis of hematopoietic stem and progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Yang,Yang Liu,Hanxiao Yin,Songbo Xie,Linlin Zhang,Xifeng Dong,Hua Ni,Weiwen Bu,Hongbo Ma,Peng Liu,Haiyan Zhu,Rongxia Guo,Lei Sun,Yue Wu,Juan Qin,Baofa Sun,Dengwen Li,Hongbo R Luo,Min Liu,Chenghao Xuan,Jun ZhouPubMed 37642636
- Nature communications 14:43602023Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees RehmanPubMed 37468519
- NAR cancer 5:zcad0322023PKM2 dictates the poised chromatin state of promoter to enhance breast cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadhura R Pandkar,Adarsh Raveendran,Kajal Biswas,Srinivas Abhishek Mutnuru,Jharna Mishra,Atul Samaiya,Tyler Malys,Alexander Y Mitrophanov,Shyam K Sharan,Sanjeev ShuklaPubMed 37388539
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e228402023P4HA2-mediated HIF-1α stabilization promotes erdafitinib-resistance in FGFR3-alteration bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuexiang Li,Yunxue Li,Bing Liu,Liang Chen,Fang Lyu,Pu Zhang,Qingliu He,Lulin Cheng,Chunyu Liu,Yarong Song,Yifei XingPubMed 36943397
- International journal of molecular sciences 24:2023Tetrathiomolybdate Decreases the Expression of Alkaline Phosphatase in Dermal Papilla Cells by Increasing Mitochondrial ROS Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Li,Hongli Liu,Xiaojing Wu,Zhicheng Song,Haojia Tang,Maohua Gong,Lei Liu,Fuchang LiPubMed 36834536
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22070842023Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyeong-Mo Koo,Chang-Dae Kim,Huijung Kim,Yeon-Woo Cho,Intan Rosalina Suhito,Tae-Hyung KimPubMed 36737855
- Nature communications 14:2882023A new AMPK isoform mediates glucose-restriction induced longevity non-cell autonomously by promoting membrane fluidity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Hyuck Jeong,Jun-Seok Han,Youngae Jung,Seung-Min Lee,So-Hyun Park,Mooncheol Park,Min-Gi Shin,Nami Kim,Mi Sun Kang,Seokho Kim,Kwang-Pyo Lee,Ki-Sun Kwon,Chun-A Kim,Yong Ryoul Yang,Geum-Sook Hwang,Eun-Soo KwonPubMed 36653384
- Cell reports 41:1118272022EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiHoon Kang,Jaemoo Chun,Jung Seok Hwang,Chaoyun Pan,Jie Li,Austin C Boese,Isabelle Young,Courteney M Malin,Yibin Kang,Don L Gibbons,Gabriel Sica,Haian Fu,Suresh S Ramalingam,Lingtao Jin,Sumin KangPubMed 36516759
- Cell reports 41:1117442022Inhibition of glutaminolysis restores mitochondrial function in senescent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDebanik Choudhury,Na Rong,Izuagie Ikhapoh,Nika Rajabian,Georgios Tseropoulos,Yulun Wu,Pihu Mehrotra,Ramkumar Thiyagarajan,Aref Shahini,Kenneth L Seldeen,Bruce R Troen,Pedro Lei,Stelios T AndreadisPubMed 36450260
- Oxidative medicine and cellular longevity 2022:49388112022MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIF Axis Suppression in the Warburg Effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Han,Yili Zhang,Bin Zhao,Jianren Yue,Zhenghong Chen,Guangyan Lei,Chen Huang,Wenjuan ChenPubMed 36304962
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Range
- 0.01 - 10 nmol/well
- Assay time
- 30m
- Sensitivity
- = 0.01 nmol/well
What's included?
Recommended products
Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Range
- 0.01 - 10 nmol/well
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 0.01 nmol/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.
Alpha Ketoglutarate assay protocol summary:
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K677 Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit. K677-100 is the same size as the 100 test size of ab83431.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-ketoglutarate sometimes called alpha-ketoglutaric acid or A-KG is an important intermediate in several metabolic processes. It plays a mechanical role in the Krebs cycle also known as the citric acid cycle where it functions to transfer energy through oxidative reactions. A-KG is a five-carbon molecule with a mass of approximately 146.11 g/mol and is primarily expressed in the mitochondria of cells particularly within tissues with high metabolic activity such as liver and muscle.
- Biological function summary
Alpha-ketoglutarate supports amino acid metabolism and the regulation of gene expression. It acts as an essential nitrogen transporter in biochemical pathways. A-KG serves as a co-substrate for several dioxygenases including prolyl hydroxylases which are enzymes connected to collagen synthesis and the hypoxia-inducible factor pathway. Additionally it is involved in the formation of neurotransmitters and the detoxification of ammonia in animals.
- Pathways
Alpha-ketoglutarate is deeply integrated within the tricarboxylic acid cycle and the gamma-aminobutyric acid (GABA) shunt. Within the TCA cycle A-KG forms important energy carriers like NADH and FADH2 impacting energy production. In the GABA shunt it closely associates with glutamate dehydrogenase which transforms glutamate emphasizing its role in nitrogen and energy balance. A-KG interacts with proteins like succinyl-CoA synthetase and is linked with enzymes such as isocitrate dehydrogenase.
- Associated diseases and disorders
Alterations in alpha-ketoglutarate levels have been linked to metabolic disorders like hyperammonemia and certain types of cancer. In hyperammonemia improper detoxification processes through the urea cycle can lead to elevated ammonia levels a problem in which alpha-ketoglutarate plays a detoxifying role. Oncogenic mutations in isocitrate dehydrogenase can lead to abnormal A-KG levels contributing to cancer progression by affecting cellular metabolism and gene expression.
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Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (ab83431)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (ab83431)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Product protocols
- Visit the general protocols
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