Ammonia Assay Kit

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Lab

Functional Studies - Ammonia Assay Kit (AB83360)

Ammonia assay performed with mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

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Supplier Data

Functional Studies - Ammonia Assay Kit (AB83360)

Ammonia initiates a cascade of reactions ultimately reacting with OxiRed, resulting in a colorimetric product with absorbance at 570 nm.

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Supplier Data

Functional Studies - Ammonia Assay Kit (AB83360)

Example of ammonia assay standard curve using ab83360

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Lab

Functional Studies - Ammonia Assay Kit (AB83360)

Ammonia assay performed in cell lysates, background signal subtracted (duplicates +/- SD).

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Lab

Functional Studies - Ammonia Assay Kit (AB83360)

Ammonia assay performed with cell culture medium (range of dilution 1 : 10-1 : 200) and control medium (range of dilution 1 : 1-1 : 20), background signal subtracted (duplicates +/- SD).

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Unknown

Functional Studies - Ammonia Assay Kit (AB83360)

Kyle Wilson et al. used ammonia assay kit ab83360 to examine ammonia levels in liver tissue in response to P. chabaudi infection. They found that in IL-10 KO mice, ammonia levels were not elevated above WT during P. chabaudi infection.

WT and IL-10 KO mice were infected with P. chabaudi and monitored during the peak of infection. WT mice were sacrificed at the peak of infection (day 10 p.i.) and IL-10 KO mice upon severe morbidity as determined via SHIRPA score.

Wilson, Kyle D et al., Journal of neuroinflammation vol. 15,1 173., Additional file 2

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Lab

Functional Studies - Ammonia Assay Kit (AB83360)

Ammonia assay performed in biological fluids (range of dilution 1 : 1-1 : 100), background signal subtracted (duplicates +/- SD).

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Unknown

Functional Studies - Ammonia Assay Kit (AB83360)

Zhiyuan Li et al. used ammonia assay kit ab83360 to examine the build-up of ammonia in old cell culture medium containing glutamine and how this might induce autophagy in cultured cells.

The ammonia assay was used to measure the ammonia concentration in cell culture medium containing Glutamine (medium alone, without cells) which was stored at 4°C or at 37°C for between 1-7 days. They found that ammonia levels increased significantly in the medium stored at 37°C.

Li, Zhiyuan et al., PloS one vol. 11,4 e0153526., Fig 1

• Schematic Diagram

Supplier Data

Schematic Diagram - Ammonia Assay Kit (AB83360)

Representative image of Ammonia Assay Kit ab83360

Components shown from left to right :

- OxiRed Probe

- Ammonium Standard I

- Developer Mix A

- Converter Mix A

- Developer III

- Assay Buffer 14

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.