Ammonia Assay Kit

Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay for ammonia and ammonium.

In the ammonia assay protocol, ammonia and ammonium are converted to a product that reacts with a probe to generate color (λ_max = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 μM) of total ammonia and ammonium, which is much more sensitive than measuring ammonia with a NADPH based assay.

Ammonia assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37°C
- analyze with microplate reader

Technical Note: A dilution series should be performed with urine samples to ensure elimination of any potential matrix effects.

This product is manufactured by BioVision, an Abcam company and was previously called K370 Ammonia Colorimetric Assay Kit. K370-100 is the same size as the 100 test size of ab83360.

Ammonia assays measure the total level of the ammonium ion and of ammonia in a sample.

At a physiological pH, nearly all ammonia exists as ammonium in solution.

The chemical equation that drives the relationship between ammonia and ammonium is: NH3 + H2O ↔ NH4+ + OH-

When the pH is low, the reaction is driven to the right, and when the pH is high, the reaction is driven to the left. In general, at around room temperature, at a pH less than 6.0, the proportion of ammonium-N plus ammonia-N as NH3 is very low and as NH4+ is very high. At a pH around 8.0 (the ammonia assay buffer pH), the proportion as NH3 is 10 percent or less.

This is our most popular ammonia assay kit; ammonia assay kit Ammonia Assay Kit - Modified Berthelot - (Colorimetric) ab102509 uses an alternative non-enzymatic method.

How other researchers have used Ammonia Assay Kit ab83360

The ammonia assay kit has been used in publications in a variety of sample types, including:
- Human: A549, 293T and U2OS cell culture lysates¹; A549 cell culture medium²
- Mouse: brain, liver tissue and serum³; plasma⁴; pancreas, kidney, liver, brain, and muscle tissue⁵; faeces⁶
- Bacteria: B. pertussis cultures⁷
- Cell culture medium⁸

^{References: 1 - Xu S et al 2019, Spinelli JB et al 2017, Trempolec N et al 2017; 2 - Jin L et al 2018; 3 - Wilson et al 2018; 4 - Khoja S et al 2018, Cantero et al 2016; 5 - Gutierrez-de-Jua V et al 2017; 6 - Shen et al 2016; 7 - Fyson et al 2017; 8 - Li et al 2016}