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AB102509

Ammonia Assay Kit - Modified Berthelot - (Colorimetric)

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(7 Publications)

Ammonia Assay Kit (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay using a modified Berthelot reagent to measure ammonia.

- Ready-to-use solutions
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
3 Images
Functional Studies - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)
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Lab

Functional Studies - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)

Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.

Functional Studies - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)
  • FuncS

Lab

Functional Studies - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted.

Schematic Diagram - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (AB102509)

Representative image of Ammonia Assay Kit - Modified Berthelot - (Colorimetric) ab102509

Components shown from left to right :

- Ammonium Standard II

- Reagent III

- Reagent II

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Sample types

Urine, Plasma, Cell culture media, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

< 10 µM

Assay time

30m

Assay Platform

Microplate reader

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Product details

Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.

How the assay works
This ammonia assay using a modified Berthelot reagent is based on a reaction between ammonia and an alkaline reagent that produces a blue-green colored product. The intensity of the color is proportional to the amount of ammonia in the sample. The colored product is indophenol, which is easily quantifiable by colorimetry (λ=670nm) using a plate reader.

Berthelot ammonia assay protocol summary
- Add samples and standards to wells
- Add reagent 1 and reagent 2 and incubate for 30 min at at 37°C
- Analyze with a microplate reader

How other researchers are using ab102509
Ammonia Assay Kit - Modified Berthelot (Colorimetric) has been used with a variety of sample types including:
Escherichia coli BL21(DE3) cells 1
Ureaplasma cells 2
Human iPS(Foreskin) 3
References: 1-Srinivasachari S et al. 2023, 2-Fleming D et al. 2022, 3-Pettinato G et al. 2021

Related and recommended products
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.

Other notes
This product was previously called K470 Biovision Ammonia Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102509-100Test", "assetComponentDetails": [ { "size":"1 x 4 mL", "name":"Reagent III", "number":"AB102509-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Reagent II", "number":"AB102509-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Ammonium Standard II", "number":"AB102509-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ammonia a small molecule with a molecular mass of about 17 daltons is an important player in nitrogen metabolism. It exists primarily as ammonium (NH4+) in physiological pH environments. Ammonia is found predominantly in liver cells where it is produced through amino acid deamination and is further involved in urea production. Ammonia detection can be performed using various tools such as the ammonia detection kit ammonia test kit and the ammonia measurement reader which are essential for researchers to analyze and quantify its presence.
Biological function summary

Ammonia participates in the nitrogen cycle being a substrate for urea synthesis in the liver a vital part of nitrogen waste removal. It combines with carbon dioxide to form carbamoyl phosphate which enters the urea cycle a complex enzymatic system within mitochondria and cytosol. Ammonia's role is not isolated; it interacts with enzymes such as glutamine synthetase. The ammonium assay and ammonia assay provide insights into ammonia's role and concentration helping elucidate its biological functions and impact.

Pathways

Ammonia is important in the urea cycle and gluconeogenesis. In the urea cycle it is converted to urea preventing toxic accumulation. Gluconeogenesis involves ammonia as it aids in converting pyruvate to glucose a fundamental process for energy maintenance. Related proteins in these pathways include carbamoyl phosphate synthetase in the urea cycle and pyruvate carboxylase in gluconeogenesis. A colorimetric assay might be used to evaluate ammonia's integration in these pathways.

Ammonia plays a significant role in hepatic encephalopathy and hyperammonemia both linked with improper ammonia metabolism. Excess ammonia in the blood due to liver dysfunction can lead to hepatic encephalopathy affecting brain function. Glutamine synthetase activity correlates to ammonia levels impacting the severity of these conditions. Ammonia assay kits are fundamental tools in assessing the association of ammonia with these disorders facilitating better understanding and management of the conditions.
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Product protocols

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Target data

See full target information Ammonia
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38674850

2024

In Vitro Fermentation Shows Polyphenol and Fiber Blends Have an Additive Beneficial Effect on Gut Microbiota States.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan A Whitman,Laurel A Doherty,Ida G Pantoja-Feliciano de Goodfellow,Kenneth Racicot,Danielle J Anderson,Katherine Kensil,J Philip Karl,Glenn R Gibson,Jason W Soares

Plants (Basel, Switzerland) 12: PubMed36679084

2023

The Arabidopsis LHT1 Amino Acid Transporter Contributes to -Mediated Plant Growth Promotion by Modulating Bacterial Metabolism in the Rhizosphere.

Applications

Unspecified application

Species

Unspecified reactive species

Israel D K Agorsor,Brian T Kagel,Cristian H Danna

PloS one 18:e0271654 PubMed36598911

2023

A dimer between monomers and hexamers-Oligomeric variations in glucosamine-6-phosphate deaminase family.

Applications

Unspecified application

Species

Unspecified reactive species

Sathya Srinivasachari,Vikas R Tiwari,Tripti Kharbanda,Ramanathan Sowdamini,Ramaswamy Subramanian

Microbiology spectrum 10:e0194221 PubMed35171026

2022

Contribution of Uremia to -Induced Hyperammonemia.

Applications

Unspecified application

Species

Unspecified reactive species

Derek Fleming,Scott A Cunningham,Robin Patel

Science advances 7: PubMed34407934

2021

Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Pettinato,Mark F Coughlan,Xuejun Zhang,Liming Chen,Umar Khan,Maria Glyavina,Conor J Sheil,Paul K Upputuri,Yuri N Zakharov,Edward Vitkin,Antonino B D'Assoro,Robert A Fisher,Irving Itzkan,Lei Zhang,Le Qiu,Lev T Perelman

Scientific reports 9:13209 PubMed31520074

2019

Fluxomic Analysis Reveals Central Carbon Metabolism Adaptation for Diazotroph Azotobacter vinelandii Ammonium Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wu,Ryan A Herold,Eric P Knoshaug,Bo Wang,Wei Xiong,Lieve M L Laurens

Microbial cell factories 18:139 PubMed31426802

2019

Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli.

Applications

Unspecified application

Species

Unspecified reactive species

Sarada S Raghavan,Sharon Chee,Juntao Li,Jeremie Poschmann,Niranjan Nagarajan,Siau Jia Wei,Chandra S Verma,Farid J Ghadessy
View all publications
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