AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples.
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- Cell death & disease 14:4132023IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia Domínguez-Zorita,Inés Romero-Carramiñana,Fulvio Santacatterina,Pau B Esparza-Moltó,Carolina Simó,Araceli Del-Arco,Cristina Núñez de Arenas,Jorge Saiz,Coral Barbas,José M CuezvaPubMed 37433784
- Oxidative medicine and cellular longevity 2022:61961732022Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Long Zhang,Pang-Bo Li,Xiao Han,Bo Zhang,Hui-Hua LiPubMed 35602095
- Developmental cell 56:811-825.e62021Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanchun Yang,Dejiang Pang,Mina Chen,Chongyangzi Du,Lanlan Jia,Luoling Wang,Yunling He,Wanxiang Jiang,Liping Luo,Zongyan Yu,Mengqian Mao,Qiuyun Yuan,Ping Tang,Xiaoqiang Xia,Yiyuan Cui,Bo Jing,Alexander Platero,Yanhui Liu,Yuquan Wei,Paul F Worley,Bo XiaoPubMed 33725483
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
What's included?
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AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Assay Platform
- Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples.
The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 uM in various sample types.
Other Notes
This product was previously called K229 Biovision AMP Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Functional Studies - AMP Assay Kit (Colorimetric) (ab273275)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Functional Studies - AMP Assay Kit (Colorimetric) (ab273275)
Measurement of AMP in different cell lysates: Jurkat (20 μg), HeLa (30 μg) and HEK293 (60 μg).
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