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AB273275

AMP Assay Kit (Colorimetric)

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(5 Publications)

AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples.
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Functional Studies - AMP Assay Kit (Colorimetric) (AB273275)
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Supplier Data

Functional Studies - AMP Assay Kit (Colorimetric) (AB273275)

Measurement of AMP in different cell lysates : Jurkat (20 μg), HeLa (30 μg) and HEK293 (60 μg).

Functional Studies - AMP Assay Kit (Colorimetric) (AB273275)
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Supplier Data

Functional Studies - AMP Assay Kit (Colorimetric) (AB273275)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Assay Platform

Microplate (12 x 8 well strips)

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Product details

AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples.

The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 uM in various sample types.

Other Notes
This product was previously called K229 Biovision AMP Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273275-100Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Developer Mix C", "number":"AB273275-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"AMP", "number":"AB273275-CMP05", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB273275-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix Q", "number":"AB273275-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 15", "number":"AB273275-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"AMP Substrate Mix", "number":"AB273275-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Adenosine Phosphate Disodium
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 70:103079 PubMed38359747

2024

Endurance exercise preconditioning alleviates ferroptosis induced by doxorubicin-induced cardiotoxicity through mitochondrial superoxide-dependent AMPKα2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Yang Qiao,Jingzhi Yu,Qihao Wang,Xinyu Wu,Qiqi Cao,Zeyu Zhang,Zhen Feng,Huan He

Cell death & disease 14:413 PubMed37433784

2023

IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Domínguez-Zorita,Inés Romero-Carramiñana,Fulvio Santacatterina,Pau B Esparza-Moltó,Carolina Simó,Araceli Del-Arco,Cristina Núñez de Arenas,Jorge Saiz,Coral Barbas,José M Cuezva

Cancer immunology, immunotherapy : CII 72:2283-2297 PubMed36881132

2023

Targeting CD73 increases therapeutic response to immunogenic chemotherapy by promoting dendritic cell maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Shan Lin,Shu-Fen Chiang,Chia-Yi Chen,Wei-Ze Hong,Tsung-Wei Chen,William Tzu-Liang Chen,Tao-Wei Ke,Pei-Chen Yang,Ji-An Liang,An-Cheng Shiau,K S Clifford Chao,Kevin Chih-Yang Huang

Oxidative medicine and cellular longevity 2022:6196173 PubMed35602095

2022

Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Long Zhang,Pang-Bo Li,Xiao Han,Bo Zhang,Hui-Hua Li

Developmental cell 56:811-825.e6 PubMed33725483

2021

Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wanchun Yang,Dejiang Pang,Mina Chen,Chongyangzi Du,Lanlan Jia,Luoling Wang,Yunling He,Wanxiang Jiang,Liping Luo,Zongyan Yu,Mengqian Mao,Qiuyun Yuan,Ping Tang,Xiaoqiang Xia,Yiyuan Cui,Bo Jing,Alexander Platero,Yanhui Liu,Yuquan Wei,Paul F Worley,Bo Xiao
View all publications
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Product promise

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