AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples.
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- Cell death & disease 14:4132023IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia Domínguez-Zorita,Inés Romero-Carramiñana,Fulvio Santacatterina,Pau B Esparza-Moltó,Carolina Simó,Araceli Del-Arco,Cristina Núñez de Arenas,Jorge Saiz,Coral Barbas,José M CuezvaPubMed 37433784
- Oxidative medicine and cellular longevity 2022:61961732022Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Long Zhang,Pang-Bo Li,Xiao Han,Bo Zhang,Hui-Hua LiPubMed 35602095
- Developmental cell 56:811-825.e62021Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanchun Yang,Dejiang Pang,Mina Chen,Chongyangzi Du,Lanlan Jia,Luoling Wang,Yunling He,Wanxiang Jiang,Liping Luo,Zongyan Yu,Mengqian Mao,Qiuyun Yuan,Ping Tang,Xiaoqiang Xia,Yiyuan Cui,Bo Jing,Alexander Platero,Yanhui Liu,Yuquan Wei,Paul F Worley,Bo XiaoPubMed 33725483
Key facts
- Detection method
Colorimetric
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Quantitative
What's included?
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AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Quantitative
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples.
The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 μM in various sample types.
This product is manufactured by BioVision, an Abcam company and was previously called K229 AMP Colorimetric Assay Kit. K229-100 is the same size as the 100 test size of ab273275.
Product promise
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Terms & Conditions.
2 product images
Functional Studies - AMP Assay Kit (Colorimetric) (ab273275)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Functional Studies - AMP Assay Kit (Colorimetric) (ab273275)
Measurement of AMP in different cell lysates: Jurkat (20 μg), HeLa (30 μg) and HEK293 (60 μg).
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