Amylase Assay Kit (Colorimetric) ab102523 detects activity of ?-amylase through a two-step reaction.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
- Molecular medicine (Cambridge, Mass.) 29:1492023Galantamine ameliorates experimental pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDane A Thompson,Tea Tsaava,Arvind Rishi,Sam J George,Tyler D Hepler,Daniel Hide,Valentin A Pavlov,Michael Brines,Sangeeta S Chavan,Kevin J TraceyPubMed 37907853
- Nutrients 15:2023Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan LiPubMed 37892502
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
40m
- Sensitivity
> 0.2 mU/well
Target data
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Alternative names
Alpha-amylase 1A, Salivary alpha-amylase, AMY1A, AMY1
What's included?
Recommended products
Amylase Assay Kit (Colorimetric) ab102523 detects activity of ?-amylase through a two-step reaction.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Alpha-amylase 1A, Salivary alpha-amylase, AMY1A, AMY1
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.2 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Amylase Assay Kit (Colorimetric) ab102523 detects activity of α-amylase through a two-step reaction.
In the amylase assay protocol, alpha-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. This causes the release of a chromophore that can then be measured at OD = 405 nm.
The assay can detect α-amylase content as low as 0.2 mU.
Amylase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader every 2-3 min for 30-60 min
This product is manufactured by BioVision, an Abcam company and was previously called K711 Amylase Activity Colorimetric Assay Kit. K711-100 is the same size as the 100 test size of ab102523.
Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes.
Supplementary info
- Activity summary
Amylase also known as alpha-amylase when referring to its most common form is an enzyme that plays an important role in the digestion of carbohydrates. It mechanically functions by catalyzing the hydrolysis of starch into sugars. Amylase is expressed heavily in the salivary glands and pancreas with pancreatic amylase having a molecular weight of approximately 50 kDa. These tissues produce amylase that gets released into the digestive tract where it continues the process of breaking down complex carbohydrates.
- Biological function summary
Amylase is integral to the digestive process by contributing to the breakdown of starches necessary for nutrient absorption. It exists mainly as a single chain protein but in some organisms it can be part of larger multi-enzyme complexes. Its enzymatic activity significantly affects how well organisms obtain energy from their food sources. Several amylase activity assays have been developed to measure how active the enzyme is in various conditions providing insights into its role in metabolism and other biological processes.
- Pathways
The function of amylase fits into the carbohydrate digestion and absorption pathway. This metabolic pathway involves other critical enzymes such as maltase and glucosidase which further break down sugar molecules. Amylase activity is key in the early stages of the pathway where it breaks down large polysaccharides into maltose and dextrins. Understanding this pathway can provide insights into nutrient absorption and energy production in organisms.
- Associated diseases and disorders
Altered amylase levels connect to conditions such as pancreatitis and salivary gland dysfunction. Pancreatic amylase levels may rise in response to acute pancreatitis providing an important biomarker for diagnosing this condition. In these diseases amylase interacts with other enzymes like lipase which can also show altered activity to indicate changes in pancreatic health. Monitoring amylase activity through amylase assay kits and protocols can offer essential information for clinicians dealing with digestive and metabolic disorders.
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5 product images
Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline or one of three drugs. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no statistically significant changes in plasma amylase activity in mice that were administered with two of the drugs vs mice administered saline. However, administration of the third to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.
Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Sample Test
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