Amylase Assay Kit (Colorimetric)

• FuncS

Lab

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.

Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.

• FuncS

PubMed

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline or one of three drugs. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

There were no statistically significant changes in plasma amylase activity in mice that were administered with two of the drugs vs mice administered saline. However, administration of the third to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).

Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Supplier Data

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

Sample Test

• FuncS

Lab

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• Schematic Diagram

Supplier Data

Schematic Diagram - Amylase Assay Kit (Colorimetric) (AB102523)

Representative image of Amylase Assay Kit (Colorimetric) ab102523

Components shown from left to right :

- Nitrophenol Standard

- Amylase Positive Control

- Amylase Substrate Mix

- Amylase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.