Amylase Assay Kit (Colorimetric) ab102523 detects activity of alpha-amylase through a two-step reaction. Readout on any colorimetric (405 nm) plate reader.
- Cited in > 50 publications
- Kit includes standard curve for quantitation, and amylase positive control
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Publications
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- Molecular medicine (Cambridge, Mass.) 29:1492023Galantamine ameliorates experimental pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDane A Thompson,Tea Tsaava,Arvind Rishi,Sam J George,Tyler D Hepler,Daniel Hide,Valentin A Pavlov,Michael Brines,Sangeeta S Chavan,Kevin J TraceyPubMed 37907853
- Nutrients 15:2023Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan LiPubMed 37892502
- Aquaculture nutrition 2023:88877682023Sustainable Aquafeed Formulations Containing Insect Larval Meal and Grape Marc for the New Zealand Farmed Abalone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Bullon,Andrea C Alfaro,Moganakumaar Manivannan,Seyedehsara Masoomi Dezfooli,Ali SeyfoddinPubMed 37885797
- Frontiers in immunology 14:11662122023Optogenetic stimulation of the brainstem dorsal motor nucleus ameliorates acute pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDane A Thompson,Tea Tsaava,Arvind Rishi,Sandeep Nadella,Lopa Mishra,David A Tuveson,Valentin A Pavlov,Michael Brines,Kevin J Tracey,Sangeeta S ChavanPubMed 37180135
- Autophagy 19:1982-19962023Copper-dependent autophagic degradation of GPX4 drives ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Xue,Ding Yan,Xi Chen,Xiaofen Li,Rui Kang,Daniel J Klionsky,Guido Kroemer,Xin Chen,Daolin Tang,Jinbao LiuPubMed 36622894
- Autophagy 19:1733-17442022Extracellular SQSTM1 exacerbates acute pancreatitis by activating autophagy-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangchun Yang,Fanghua Ye,Jiao Liu,Daniel J Klionsky,Daolin Tang,Rui KangPubMed 36426912
- American journal of physiology. Gastrointestinal and liver physiology 323:G375-G3862022Urolithin A attenuates severity of chronic pancreatitis associated with continued alcohol intake by inhibiting PI3K/AKT/mTOR signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiddharth Mehra,Supriya Srinivasan,Samara Singh,Zhiqun Zhou,Vanessa Garrido,Iago De Castro Silva,Tulasigeri M Totiger,Austin R Dosch,Xizi Dai,Rajinder K Dawra,Venkatakrishna Rao Jala,Chanjuan Shi,Jashodeep Datta,Michael VanSaun,Nipun Merchant,Nagaraj NagathihalliPubMed 36098401
- Frontiers in physiology 13:9345972022Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsmaa Mohammed ShamsEldeen,Sarah A Abd El-Aal,Basma Emad Aboulhoda,Hend AbdAllah,Sara Mahmoud Gamal,Fatma E Hassan,Marwa Nagi Mehesen,Laila Ahmed Rashed,Abeer Mostafa,Nermeen Bakr SadekPubMed 35812319
- Autophagy 19:54-742022Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Chen,Xinxin Song,Jingbo Li,Ruoxi Zhang,Chunhua Yu,Zhuan Zhou,Jiao Liu,Siyan Liao,Daniel J Klionsky,Guido Kroemer,Jinbao Liu,Daolin Tang,Rui KangPubMed 35403545
- Frontiers in immunology 13:8616702022Negative Immune Checkpoint Protein, VISTA, Regulates the CD4 T Population During Sepsis Progression to Promote Acute Sepsis Recovery and Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChyna C Gray,Bethany Biron-Girard,Michelle E Wakeley,Chun-Shiang Chung,Yaping Chen,Yael Quiles-Ramirez,Jessica D Tolbert,Alfred AyalaPubMed 35401514
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Assay time
- 40m
- Sensitivity
- > 0.2 mU/well
Target data
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Alternative names
AMY1, AMY1A, Alpha-amylase 1A, Salivary alpha-amylase
What's included?
Recommended products
Amylase Assay Kit (Colorimetric) ab102523 detects activity of alpha-amylase through a two-step reaction. Readout on any colorimetric (405 nm) plate reader.
- Cited in > 50 publications
- Kit includes standard curve for quantitation, and amylase positive control
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Assay time
- 40m
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.2 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In the amylase assay protocol, alpha-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. This causes the release of a chromophore that can then be measured at OD = 405 nm.
The assay can detect α-amylase content as low as 0.2 mU.
Amylase assay protocol summary:
- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD405 nm) in a kinetic mode at 25°C every 2-3 min for 30-60 min on a microplate reader
How other researchers are using
Amylase Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse blood serum 1
Mouse pancreas samples 2
Human pancreatic cancer cell lines 3
References:
1-Thompson D et al. 2023
2-Thompson D et al. 2023
3-Xue Q et al. 2023
Related and recommended products
To screen potential inhibitors of Alpha-Amylase we recommend:
- Alpha-Amylase Inhibitor Screening Kit (Colorimetric) Alpha-Amylase Inhibitor Screening Kit (Colorimetric) ab283391
This product is manufactured by BioVision, an Abcam company and was previously called K711 Amylase Activity Colorimetric Assay Kit. K711-100 is the same size as the 100 test size of ab102523.
Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Amylase also known as alpha-amylase when referring to its most common form is an enzyme that plays an important role in the digestion of carbohydrates. It mechanically functions by catalyzing the hydrolysis of starch into sugars. Amylase is expressed heavily in the salivary glands and pancreas with pancreatic amylase having a molecular weight of approximately 50 kDa. These tissues produce amylase that gets released into the digestive tract where it continues the process of breaking down complex carbohydrates.
- Biological function summary
Amylase is integral to the digestive process by contributing to the breakdown of starches necessary for nutrient absorption. It exists mainly as a single chain protein but in some organisms it can be part of larger multi-enzyme complexes. Its enzymatic activity significantly affects how well organisms obtain energy from their food sources. Several amylase activity assays have been developed to measure how active the enzyme is in various conditions providing insights into its role in metabolism and other biological processes.
- Pathways
The function of amylase fits into the carbohydrate digestion and absorption pathway. This metabolic pathway involves other critical enzymes such as maltase and glucosidase which further break down sugar molecules. Amylase activity is key in the early stages of the pathway where it breaks down large polysaccharides into maltose and dextrins. Understanding this pathway can provide insights into nutrient absorption and energy production in organisms.
- Associated diseases and disorders
Altered amylase levels connect to conditions such as pancreatitis and salivary gland dysfunction. Pancreatic amylase levels may rise in response to acute pancreatitis providing an important biomarker for diagnosing this condition. In these diseases amylase interacts with other enzymes like lipase which can also show altered activity to indicate changes in pancreatic health. Monitoring amylase activity through amylase assay kits and protocols can offer essential information for clinicians dealing with digestive and metabolic disorders.
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5 product images
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.
Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.
Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline or one of three drugs. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no statistically significant changes in plasma amylase activity in mice that were administered with two of the drugs vs mice administered saline. However, administration of the third to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.
Functional Studies - Amylase Assay Kit (Colorimetric) (ab102523)
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