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AB204726

Angiogenesis Assay Kit (In Vitro)

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(40 Publications)

Angiogenesis Assay Kit / Tube Formation Assay Kit ab204726 provides a quick and robust method to measure the ability of endothelial cells to form three-dimensional tube-like structures in vitro in less than 18 hours.

- Qualitative tool for assessing angiogenesis in a 96-well plate assay
- Includes ECM, buffer, control, staining dye.
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Angiogenesis Assay Kit (In Vitro) (AB204726)
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Supplier Data

Functional Studies - Angiogenesis Assay Kit (In Vitro) (AB204726)

Endothelial Cell (EA.hy926 Cells) Tube Formation.

Phase contrast (a, c, e) and fluorescent images (b, d, f) of endothelial cells in a tissue culture plate. (a, b) Endothelial cells grown without the Extracellular Matrix Gel, (c, d) Tube formation of endothelial cells grown on Extracellular Matrix gel. (e, f) endothelial cells grown on Extracellular Matrix gel treated with Suramin (10 μmol/L). Images were taken using Nikon TE2000 microscope.

ELISA - Angiogenesis Assay Kit (In Vitro) (AB204726)
  • ELISA

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ELISA - Angiogenesis Assay Kit (In Vitro) (AB204726)

HUVEC morphogenesis on Extracellular Matrix Gel. Cells (2 × 104) were plated per 1 cm2 well precoated with Extracellular Matrix Gel and grown for 18 hours (A) in the specific medium alone (positive control) or containing (B) PMA 10 μmol/L.

Other - Angiogenesis Assay Kit (In Vitro) (AB204726)
  • Other

Supplier Data

Other - Angiogenesis Assay Kit (In Vitro) (AB204726)

Representative image of Angiogenesis Assay Kit (In Vitro) ab204726

Components shown from left to right :

- Inhibitor (Suramin)

- Staining Dye Concentrate

- Extracellular Matrix Solution (2 vials)

- Elution Buffer I

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts

Assay type

Cell-based

Results type

Qualitative

Assay time

6h

Assay Platform

Fluorescence microscope

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Product details

Angiogenesis assays can help determine which compounds promote or inhibit angiogenesis, and can provide insight into the role of angiogenesis in disease.

Angiogenesis assay / tube formation assay protocol summary

- Add extracellular matrix solution to empty culture plate and incubate for 1 hr at 37°C to allow the solution to form a gel
- Plate cells onto the gel and add experimental treatment
- Incubate cells for 4-18 hrs to allow tube formation
- Remove incubation medium and wash cells / gel
- Add staining dye and incubate for 30 min
- Examine tube formation using light and fluorescence microscopy (green filter)

This product is manufactured by BioVision, an Abcam company and was previously called K905 Angiogenesis (Tube Formation) Assay. K905-50 is the same size as the 50 test size of ab204726.

Angiogenesis is a physiological process that occurs during wound healing and normal development which involves the growth of new blood vessels from pre-existing vessels. These blood vessels form highly branched, tree-like tubular networks that ensure efficient and simultaneous transport of gases, liquids, nutrients, signaling molecules, and circulating cells between tissues and organs. Angiogenesis is complex and highly regulated, with tight coordination of cell proliferation, differentiation, migration, matrix adhesion, and cell-to-cell signaling. Angiogenesis is regulated by several factors, most importantly growth factors such as vascular endothelial growth factors (VEGFs) and platelet-derived growth factors (PDGFs).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab204726-50Test", "assetComponentDetails": [ { "size":"2 x 1.25 mL", "name":"Extracellular Matrix Solution", "number":"AB204726-CMP01", "productcode":"" }, { "size":"1 x 25 µL", "name":"Staining Dye Concentrate (Sterile)", "number":"AB204726-CMP03", "productcode":"" }, { "size":"1 x 1 Each", "name":"Inhibitor (Suramin)", "number":"AB204726-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Elution Buffer I", "number":"AB204726-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tube formation also referred to as endothelial tube formation describes a process where endothelial cells create capillary-like structures. This process occurs in vitro during angiogenic assays and serves as a vital component in angiogenesis the growth of new blood vessels from existing ones. The endothelial tube formation can be observed using various in vitro assay methods including the use of the HUVEC tube formation assay. Endothelial cells express specific proteins on their surface that mediate adhesion and growth signals essential for this process. These cells typically weigh about 120 kDa and are predominantly expressed in blood vessel-lining tissues.
Biological function summary

Endothelial cells are responsible for the formation of tubular networks that provide the structural framework for vascular systems. These cells secrete factors that enable the remodeling and stabilization of new blood vessels. The process of tube formation involves the collaboration of multiple complexes including the integrin complexes which facilitate cell adhesion and migration necessary for angiogenesis. During in vitro assays endothelial tube formation assays use unique environments created by in vitro kits that mimic the extracellular matrix promoting the cells' ability to form interconnected tube structures mimicking in vivo conditions.

Pathways

Endothelial tube formation is integral within angiogenic pathways particularly the VEGF signaling pathway. Vascular endothelial growth factor (VEGF) is an important protein that stimulates endothelial cell function promoting their proliferation and migration. Another pivotal pathway is the Notch signaling pathway which works alongside VEGF signaling to facilitate the precise organization and maturation of the formed tubes. Proteins such as eNOS (endothelial nitric oxide synthase) play significant roles in these pathways by mediating responses fundamental for blood vessel formation and vascular tone regulation.

The dysfunctional regulation of endothelial tube formation is linked to pathological conditions like cancer and diabetic retinopathy. In cancer abnormal angiogenesis facilitates tumor growth and metastasis. VEGF overexpression creates an excessive network of immature vessels that supply the tumor with nutrients and oxygen. Conversely in diabetic retinopathy faulty tube formation contributes to the development of aberrant blood vessel structures leading to impaired vision. Both pathogenic processes are associated with altered expressions of factors like VEGF and integrins emphasizing the important role of endothelial tube formation in maintaining normal vascular function.
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Product protocols

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Target data

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed39575482

2024

MDM2 interacts with PTEN to inhibit endothelial cell development and promote deep vein thrombosis via the JAK/STAT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Jiao,Deng Zhang,Jianbo Peng,Yunsai Li

Journal of clinical medicine 13: PubMed39336917

2024

Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic Telangiectasia.

Applications

Unspecified application

Species

Unspecified reactive species

Yanru Wang,Linda Hofmann,Diana Huber,Robin Lochbaum,Sonja Ludwig,Cornelia Brunner,Thomas K Hoffmann,René Lehner,Marie-Nicole Theodoraki

The Journal of experimental medicine 221: PubMed39320470

2024

Mitochondria as a primary determinant of angiogenic modality in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Maki Niihori,Joel James,Mathews V Varghese,Nolan McClain,Odunayo Susan Lawal,Rohit C Philip,Brenda K Baggett,Dmitry A Goncharov,Vinicio de Jesus Perez,Elena A Goncharova,Ruslan Rafikov,Olga Rafikova

The Journal of biological chemistry 300:107807 PubMed39307302

2024

Aberrant overexpression of myosin 1b in glioblastoma promotes angiogenesis via VEGF-myc-myosin 1b-Piezo1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lv,Fan Yang,Zhengmao Ge,Lele Xin,Lingxue Zhang,Yaohong Zhai,Xian Liu,Qingdong Guo,Xinggang Mao,Peng Luo,Lei Zhang,Xiaofan Jiang,Yanyu Zhang

Pharmaceutical biology 62:153-161 PubMed38347502

2024

Effects of total coumarins from on exosomal miRNA expression and angiogenesis in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Dao-Hai Cheng,Zheng-Ying Su,Ji-Hua Lv,Li Wang,Yu-Yin Deng,Li Li

Cell reports 42:113067 PubMed37659081

2023

AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Nung Hung,Meizhen Chen,Daniel T DeArmond,Cheryl H-L Chiu,Catherine A Limboy,Xi Tan,Meena Kusi,Chih-Wei Chou,Li-Ling Lin,Zhao Zhang,Chiou-Miin Wang,Chun-Liang Chen,Kohzoh Mitsuya,Pawel A Osmulski,Maria E Gaczynska,Nameer B Kirma,Ratna K Vadlamudi,Don L Gibbons,Steve Warner,Andrew J Brenner,Daruka Mahadevan,Joel E Michalek,Tim H-M Huang,Josephine A Taverna

European journal of histochemistry : EJH 67: PubMed37132497

2023

The role of miR-143-3p/FNDC1 axis on the progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanshu Ma,Qi Gao,Wenjing Xin,Lei Wang,Yan Chen,Chang Su,Songyan Gao,Ruiling Sun

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22924 PubMed37071462

2023

Three-dimensional-printed MPBI@β-TCP scaffold promotes bone regeneration and impedes osteosarcoma under near-infrared laser irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangjie Lu,Huihan Wang,Minjie Yang,Lijun Wang,Kaifeng Gan

BMC biotechnology 23:8 PubMed36927344

2023

Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Sauter,Hannes Degenhardt,Jutta Tuebel,Peter Foehr,Philipp Knoeckel,Kira Florian,Fiona Charitou,Rainer Burgkart,Andreas Schmitt

Integrative cancer therapies 22:15347354221134513 PubMed36859800

2023

Golden Leaf Extract (GGLE) Inhibits Melanoma Cell Invasion and Angiogenesis Through Inhibition of Angiogenin.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Chen,Tao Wang,Qi Chen
View all publications
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