Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit ab273373 uses the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore.
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- Nature communications 14:36802023In vivo inhibition of nuclear ACE2 translocation protects against SARS-CoV-2 replication and lung damage through epigenetic imprinting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Juan Tu,Michelle Melino,Jenny Dunn,Robert D McCuaig,Helle Bielefeldt-Ohmann,Sofiya Tsimbalyuk,Jade K Forwood,Taniya Ahuja,John Vandermeide,Xiao Tan,Minh Tran,Quan Nguyen,Liang Zhang,Andy Nam,Liuliu Pan,Yan Liang,Corey Smith,Katie Lineburg,Tam H Nguyen,Julian D J Sng,Zhen Wei Marcus Tong,Keng Yih Chew,Kirsty R Short,Roger Le Grand,Nabila Seddiki,Sudha RaoPubMed 37369668
- Nature 615:134-1422022FXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Brevini,Mailis Maes,Gwilym J Webb,Binu V John,Claudia D Fuchs,Gustav Buescher,Lu Wang,Chelsea Griffiths,Marnie L Brown,William E Scott,Pehuén Pereyra-Gerber,William T H Gelson,Stephanie Brown,Scott Dillon,Daniele Muraro,Jo Sharp,Megan Neary,Helen Box,Lee Tatham,James Stewart,Paul Curley,Henry Pertinez,Sally Forrest,Petra Mlcochova,Sagar S Varankar,Mahnaz Darvish-Damavandi,Victoria L Mulcahy,Rhoda E Kuc,Thomas L Williams,James A Heslop,Davide Rossetti,Olivia C Tysoe,Vasileios Galanakis,Marta Vila-Gonzalez,Thomas W M Crozier,Johannes Bargehr,Sanjay Sinha,Sara S Upponi,Corrina Fear,Lisa Swift,Kourosh Saeb-Parsy,Susan E Davies,Axel Wester,Hannes Hagström,Espen Melum,Darran Clements,Peter Humphreys,Jo Herriott,Edyta Kijak,Helen Cox,Chloe Bramwell,Anthony Valentijn,Christopher J R Illingworth,Bassam Dahman,Dustin R Bastaich,Raphaella D Ferreira,Thomas Marjot,Eleanor Barnes,Andrew M Moon,Alfred S Barritt,Ravindra K Gupta,Stephen Baker,Anthony P Davenport,Gareth Corbett,Vassilis G Gorgoulis,Simon J A Buczacki,Joo-Hyeon Lee,Nicholas J Matheson,Michael Trauner,Andrew J Fisher,Paul Gibbs,Andrew J Butler,Christopher J E Watson,George F Mells,Gordon Dougan,Andrew Owen,Ansgar W Lohse,Ludovic Vallier,Fotios SampaziotisPubMed 36470304
- Pharmaceutics 14:2022Structurally Modified Bioactive Peptide Inhibits SARS-CoV-2 Lentiviral Particles Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhushwant S Bhullar,Manal A Nael,Khaled M Elokely,Steven J Drews,Jianping WuPubMed 36297481
- Biosensors 12:2022Fluorescence-Based Measurements of Membrane-Bound Angiotensin Converting Enzyme 2 Activity Using Xenopus Laevis Oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuise Fast,Richard Ågren,Hugo ZebergPubMed 36004997
- Frontiers in immunology 13:9066872022Glucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHassan Sarker,Rashmi Panigrahi,Eugenio Hardy,J N Mark Glover,Shokrollah Elahi,Carlos Fernandez-PatronPubMed 35784352
- Molecular therapy : the journal of the American Society of Gene Therapy 30:2998-30162022Identification of FDA-approved bifonazole as a SARS-CoV-2 blocking agent following a bioreporter drug screen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaid Taha,Rozanne Arulanandam,Glib Maznyi,Elena Godbout,Madalina E Carter-Timofte,Naziia Kurmasheva,Line S Reinert,Andrew Chen,Mathieu J F Crupi,Stephen Boulton,Geneviève Laroche,Alexandra Phan,Reza Rezaei,Nouf Alluqmani,Anna Jirovec,Alexandra Acal,Emily E F Fekete,Ragunath Singaravelu,Julia Petryk,Manja Idorn,Kyle G Potts,Hayley Todesco,Cini John,Douglas J Mahoney,Carolina S Ilkow,Patrick Giguère,Tommy Alain,Marceline Côté,Søren R Paludan,David Olagnier,John C Bell,Taha Azad,Jean-Simon DialloPubMed 35526097
- Cell metabolism 34:424-440.e72022Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilun Li,Meixiu Peng,Pin Chen,Chenshu Liu,Ao Hu,Yixin Zhang,Jiangyun Peng,Jiang Liu,Yihui Li,Wenxue Li,Wei Zhu,Dongxian Guan,Yang Zhang,Hongyin Chen,Jiuzhou Li,Dongxiao Fan,Kan Huang,Fen Lin,Zefeng Zhang,Zeling Guo,Hengli Luo,Xi He,Yuanyuan Zhu,Linghua Li,Bingding Huang,Weikang Cai,Lei Gu,Yutong Lu,Kai Deng,Li Yan,Sifan ChenPubMed 35150639
- Antiviral research 196:1051972021Picomolar inhibition of SARS-CoV-2 variants of concern by an engineered ACE2-IgG4-Fc fusion protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHristo L Svilenov,Julia Sacherl,Alwin Reiter,Lisa S Wolff,Cho-Chin Cheng,Marcel Stern,Vincent Grass,Martin Feuerherd,Frank-Peter Wachs,Nicole Simonavicius,Susanne Pippig,Florian Wolschin,Oliver T Keppler,Johannes Buchner,Carsten Brockmeyer,Ulrike ProtzerPubMed 34774603
- NPJ vaccines 6:1062021Haplotype-resolved de novo assembly of the Vero cell line genome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Angélique Sène,Sascha Kiesslich,Haig Djambazian,Jiannis Ragoussis,Yu Xia,Amine A KamenPubMed 34417462
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e214192021A pressor dose of angiotensin II has no influence on the angiotensin-converting enzyme 2 and other molecules associated with SARS-CoV-2 infection in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Hikari Takeshita,Koichi Yamamoto,Yibin Huang,Cheng Wang,Tsuneo Nakajima,Yoichi Nozato,Taku Fujimoto,Serina Yokoyama,Kazuhiro Hongyo,Futoshi Nakagami,Hiroshi Akasaka,Yoichi Takami,Yasushi Takeya,Ken Sugimoto,Hiromi RakugiPubMed 33566370
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Enzyme activity (quantitative)
- Sensitivity
= 400 µU
Target data
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).Isoform 2Non-functional as a carboxypeptidase.(Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.Isoform 2(Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Alternative names
Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase, ACE2, UNQ868/PRO1885
What's included?
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Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit ab273373 uses the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore.
Alternative names
Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase, ACE2, UNQ868/PRO1885
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate
- Sensitivity
= 400 µU
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit ab273373) utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. We also provide an ACE2 specific inhibitor that can differentiate the ACE2 activity from other proteolytic activity. The kit can detect as low as 0.4 mU. The assay kit is simple and can be used in a high-throughput format. A negative control should be run for each sample to differentiate ACE2 activity from other proteolytic activity in your sample
This product is manufactured by BioVision, an Abcam company and was previously called K897 Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric). K897-100 is the same size as the 100 test size of ab273297.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
- Biological function summary
The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.
- Pathways
ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.
- Associated diseases and disorders
ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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4 product images
Functional Studies - Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
MCA Standard Curve (0-300 pmol), error bars indicate SD (n=3).
Functional Studies - Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297)
Kinetic activity curves using different amounts of ACE2 Positive Control in the assay.
Functional Studies - Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297)
ACE2 Activity in Tissue Samples.
ACE2 activity was measured for different types of rat tissue samples (total protein in lung and kidney; 17 μg and 23 μg respectively), and human kidney tissue sample (10 μg total protein) in presence (+ Inhibitor) and absence (- Inhibitor) of ACE2 Inhibitor.
Functional Studies - Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (ab273297)
Spiked ACE2 activity and inhibition measured in HEK-293 cell lysate (total protein: 37 μg).
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