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AB236215

Annexin V-APC Assay Kit

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(3 Publications)

Annexin V-APC Assay Kit (ab236215) employs an APC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells.

View Alternative Names

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha

1 Images
Flow Cytometry - Annexin V-APC Assay Kit (AB236215)
  • Flow Cyt

Supplier Data

Flow Cytometry - Annexin V-APC Assay Kit (AB236215)

Ultraviolet light induces apoptosis in Jurkat cells.

Jurkat (Human T cell leukemia cell line from peripheral blood) cells were left untreated (panel A) or stimulated with 200 mJ/cm2 ultraviolet light (panel B) and then incubated for 4 hours at 37°C. Cells were stained using Annexin V APC Assay Reagent at a 1 : 200 dilution in 1X Binding Buffer for 15 minutes at RT in the dark. After centrifugation and removal of the supernatant, cells were resuspended in 1 : 100 DAPI in PBS.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells

Assay type

Cell-based (quantitative)

Assay Platform

Microplate reader, Flow cytometer

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Product details

Annexin V-APC Assay Kit (ab236215) employs an APC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. DAPI is used as a marker of cell membrane permeability seen in very late apoptotic or necrotic cells. The reagents provided are sufficient to run 100 samples when using a 96-well format.
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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab236215-100Test", "assetComponentDetails": [ { "size":"1 x 100 Test", "name":"Annexin V APC Assay Reagent", "number":"AB236215-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"DAPI Viability Dye", "number":"AB236215-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Cell-Based Assay Annexin V Binding Buffer (10X)", "number":"AB236215-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.
See full target information ANXA5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e70048 PubMed39390760

2024

Reduction of myeloid-derived suppressor cells in prostate cancer murine models and patients following white button mushroom treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Wang,Shoubao Ma,Przemyslaw Twardowski,Clayton Lau,Yin S Chan,Kelly Wong,Sai Xiao,Jinhui Wang,Xiwei Wu,Paul Frankel,Timothy G Wilson,Timothy W Synold,Cary Presant,Tanya Dorff,Jianhua Yu,David Sadava,Shiuan Chen

The Journal of clinical investigation 133: PubMed37824211

2023

Improving radiotherapy in immunosuppressive microenvironments by targeting complement receptor C5aR1.

Applications

Unspecified application

Species

Unspecified reactive species

Callum Beach,David MacLean,Dominika Majorova,Stavros Melemenidis,Dhanya K Nambiar,Ryan K Kim,Gabriel N Valbuena,Silvia Guglietta,Carsten Krieg,Mahnaz Darvish-Damavandi,Tatsuya Suwa,Alistair Easton,Lily Vs Hillson,Ashley K McCulloch,Ross K McMahon,Kathryn Pennel,Joanne Edwards,Sean M O'Cathail,Campbell S Roxburgh,Enric Domingo,Eui Jung Moon,Dadi Jiang,Yanyan Jiang,Qingyang Zhang,Albert C Koong,Trent M Woodruff,Edward E Graves,Tim Maughan,Simon Ja Buczacki,Manuel Stucki,Quynh-Thu Le,Simon J Leedham,Amato J Giaccia,Monica M Olcina

The Journal of pathology 252:304-316 PubMed32725633

2020

Phospholipase D1 inhibition sensitizes glioblastoma to temozolomide and suppresses its tumorigenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Woo Kang,Won Chan Hwang,Yu Na Noh,Kang Seo Park,Do Sik Min
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