Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX

Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144) includes Annexin V-Cy3, SYTOX green dye, and binding buffer. It can be used in a 10 min, one-step staining procedure to detect apoptosis and necrosis. Analysis is by flow cytometry or fluorescence microscopy.

The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids.

After staining a cell population with annexin V-Cy3 and SYTOX Green dye in binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.

These populations can easily be distinguished by fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).

The Annexin V-Cy3 reagent contained in the kit is also available as Annexin V-Cy3 reagent Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) ab14082.

The Annexin V apoptosis assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. For more apoptosis assays, review the full set of , or the .

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